IMP3与CD44v6在甲状腺乳头状癌中的表达及临床意义

目的 探讨IMP3及CD44v6在甲状腺乳头状癌（PTC）中的表达及其临床意义.方法 应用免疫组织化学方法检测30例甲状腺乳头状癌（甲状腺滤泡型乳头状癌10例,非滤泡型乳头状癌20例）,20例甲状腺乳头状癌并颈部淋巴结转移,20例甲状腺良性组织（10例正常组织,10例结节性甲状腺肿）中IMP3和CD44v6表达水平,检测结果用Biosens Digital Imaging System vl.6专业图像分析软件进行定量分析,选择积分光密度（IOD）作为评价参数.结果 IMP3与CD44V6在PTC中的表达高于良性组织中的表达,差异有统计学意义（P ＜0.05）;IMP3与CD44v6在PTC有淋巴结转移中的表达高于无淋巴结转移组织中的表达,差异有统计学意义（P ＜0.05）;IMP3与CD44v6在PTC中的表达呈正相关（r=0.903,P＜0.05）.结论 IMP3与CD44v6在甲状腺乳头状癌中呈高表达,有作为分子标志物协助PTC的诊断及判断PTC的侵袭力及转移力的价值.     

甲状腺乳头状癌雌孕激素受体的检测临床及生物学意义

对１０５例甲状腺良、恶性疾病和２４例正常的甲状腺组织采用了酶联法进行雌孕激素受体的检测。乳头状癌，腺瘤、甲状腺肿以及正常的甲状腺组织的雌、孕激素受体阳性分别为５８．３％和４８．３％；３１．２％和２８．１％；３０．８％和３０．７％；２０．８％和１６．７％。乳头状腺癌的阳性率明显要高于甲状腺良性病变。在乳头状腺癌中，小于４５岁年龄组的雌激素受体的的阳性率明显要高于其它甲状腺病变。在乳头状腺癌中，小于４５岁的年龄组的雌激素受体的阳性率明显高于大于或等于４５岁的年龄组。雌、孕激素受体的阳性率和性别、病理分期，肿块大小，以及颈部淋巴结受累情况无关。二倍体乳头腺癌的雌激素受体阳性率要比异倍体肿瘤受体阳性率要高。     

单片机控制的PTC热风烘干装置的研制

利用单片机控制PTC加热，研制一种高效、快速的烘干装置。     

B超及B超引导下PTC在胆系结石诊断中的对照研究

目的 评价Ｂ超与Ｂ超引导下ＰＴＣ（经皮肝穿刺胆管造影）对胆系结石病人诊断的价值。方法 对Ｂ超与Ｂ超引导下ＰＴＣ２２１例经手术及病理证实的胆系结石病人进行分析对照。结果 ＰＴＣ诊断胆总管下段结石符合率为９８．０２％,超声显像诊断胆总管下段结石符合率为８２．１８％,二者相差非常显著（Ｐ〈０．０１）。而Ｂ超对肝内胆管结石的诊断符合率是９５．２４％,ＰＴＣ诊断肝内胆管结石的诊断符合率是７１．４３％,二者相     

Expression of pendrin in benign and malignant human thyroid tissues

The Pendred syndrome gene (PDS) encodes a transmembrane protein, pendrin, which is expressed in follicular thyroid cells and participates in the apical iodide transport. Pendrin expression has been studied in various thyroid neoplasms by means of immunohistochemistry (IHC), Western blot and RT-quantitative real-time PCR. The expression was related to the functional activity of the thyroid tissue. Follicular cells of normal, nodular goitre and Graves' disease tissues express pendrin at the apical pole of the thyrocytes. In follicular adenomas, pendrin was detected in cell membranes and cytoplasm simultaneously in 10 out of 15 cases. Pendrin protein was detected in 73.3 and 76.7% of the follicular (FTC) and papillary (PTC) thyroid carcinomas, respectively, where pendrin was solely localised inside the cytoplasm. An extensive intracellular immunostaining of pendrin was observed in six out of 11 (54.5%) of positive FTCs and 19 out of 23 (82%) of PTCs. Focal reactivity was detected in one follicular- and three papillary carcinomas, whereas pendrin protein was absent in three of 15 FTC and four of 30 PTC; mRNA of pendrin was detected in 92.4% of thyroid tumours. The relative mRNA expression of pendrin was lower in cancers than in normal thyroid tissues (P<0.001). The pendrin protein level was found to parallel its mRNA expression, which was not, however, related to the tumour size and tumour stage. In conclusion, pendrin is expressed in the majority of differentiated thyroid tumours with high individual variability but its targeting to the apical cell membrane is affected.     

2-氨基异黄酮化合物的合成

本文报道了一系列2-氨基-3′-胺甲基-4′-羟基-6-取代异黄酮化合物(Ⅱ)的合成。中间体2-甲氧基-5-取代苯甲酸甲酯(Ⅲ)系用相转移催化技术同时进行醚化和酯化得到。Claisen缩合产物用三溴化硼脱甲基同时环化成2-氨基异黄酮化合物(Ⅵ)。利用Mannich反应在4′-羟基的邻位引入胺甲基制得目的物。药理筛选结果显示Ⅱbb′对小鼠具有明显的耐缺氧作用。     

Carcinoma of the extrahepatic bile ducts

Thirty-seven patients with carcinoma of the extrahepatic bile ducts (EHBD), diagnosed over 14 years from 1974 to 1987, were studied retrospectively. The most common presenting complaint was jaundice, followed by abdominal pain, nausea and vomiting, and weight loss. The majority of EHBD carcinomas were located at the middle third of the bile duct (43.2%), followed by upper third bile duct (37.8%), and lower third bile duct (18.9%). The resectability of the surgically managed group was 44.4%. The most common histologic type was adenocarcinoma. The 30-day postoperative mortality was 30.6%. The mean overall survival of the patients with EHBD carcinoma was 11.2 months. Patients with distal third tumors had the best prognosis with a mean overall survival of 16.0 months. The mean overall survival of patients with upper-third and middle-third tumors were 10.9 months and 9.4 months. A high index of suspicion of the disease as well as earlier, aggressive surgery may improve patient survival.     

Phenylthiocarbamide taste sensitivity revisited: complete sorting test supports residual family resemblance

Phenylthiocarbamide (PTC) taste thresholds were determined in 100 nuclear families using the complete sorting test. Segregation analysis using the mixed model suggested that the variability in PTC thresholds is controlled by a major locus with incomplete dominance as well as by a multifactorial component with significant residual heritability. Such a model explained nearly 96% of the variance, leaving only 4% of the variance in thresholds arising from measurement error and other environmental factors.     

超声引导下经皮肝穿胆道造影及胆管内置管引流在梗阻性黄疸中临床应用

本文报告１０例超声引导下经皮肝穿胆道造影，胆管内置管引流成功经验。１０例病人均为恶性梗阻性黄疸。成功９例，１例因肝内胆管畸形改变未成功。９例成功引流的病人均行手术治疗，术后无一例并发症。进行超声引导下行胆道造影，胆管内置管引流准确性及成功率高，明显减少术后并发症，是值得推广的一种方法。