整合素αvβ6:一种新的诊断口腔鳞状细胞癌侵袭潜能指标

目的:观察整合素αvβ6在人口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达,探讨其表达与临床病理参数之间的关系.方法:采用免疫组化及Image Pro Plus 6.0软件分析OSCC内αvβ6的IA值,并进行统计学处理.结果:癌组织的胞膜及胞浆中广泛出现αvβ6着色,而在正常口腔黏膜和良性上皮肿瘤中αvβ6不表达或弱表达.高、中及低分化癌中αvβ6的IA值分别为74.0128、132.892、259.432.αvβ6的表达在分化程度和肿瘤细胞活性方面存在统计学上的差异(P＜0.05).结论:αvβ6的表达强弱与细胞的侵袭或者异质间粘附密切相关,其表达的强弱可以作为口腔鳞状细胞癌侵袭与转移的一种指示剂.     

Improvement of Oral Bioavailability and Anti-Tumor Effect of Zingerone Self-Microemulsion Drug Delivery System

This study sought to prepare a self-microemulsion drug delivery system containing zingerone (Z-SMEDDS) to improve the low oral bioavailability of zingerone and anti-tumor effect. Z-SMEDDS was characterized by particle size, zeta potential and encapsulation efficiency, while its pharmacokinetics and anti-tumor effects were also evaluated. Z-SMEDDS had stable physicochemical properties, including average particle size of 17.29 ± 0.07 nm, the zeta potential of -22.81 ± 0.29 mV, and the encapsulation efficiency of 97.96% ± 0.02%. In vitro release studies have shown the release of zingerone released by Z-SMEDDS was significantly higher than free zingerone in different release media. The relative oral bioavailability of Z-SMEDDS was 7.63 times compared with free drug. Meanwhile, the half inhibitory concentration （IC50）of Z-SMEDDS and free zingerone was 8.45 μg/mL and 13.30 μg/mL, respectively on HepG2. This study may provide a preliminary basis for further clinical research and application of Z-SMEDDS.     

A model evaluation study for treatment planning of laser-induced thermal therapy

Abstract

A cross-validation analysis evaluating computer model prediction accuracy for a priori planning magnetic resonance-guided laser-induced thermal therapy (MRgLITT) procedures in treating focal diseased brain tissue is presented. Two mathematical models are considered. (1) A spectral element discretisation of the transient Pennes bioheat transfer equation is implemented to predict the laser-induced heating in perfused tissue. (2) A closed-form algorithm for predicting the steady-state heat transfer from a linear superposition of analytic point source heating functions is also considered. Prediction accuracy is retrospectively evaluated via leave-one-out cross-validation (LOOCV). Modelling predictions are quantitatively evaluated in terms of a Dice similarity coefficient (DSC) between the simulated thermal dose and thermal dose information contained within N?=?22 MR thermometry datasets. During LOOCV analysis, the transient model’s DSC mean and median are 0.7323 and 0.8001 respectively, with 15 of 22 DSC values exceeding the success criterion of DSC?≥?0.7. The steady-state model’s DSC mean and median are 0.6431 and 0.6770 respectively, with 10 of 22 passing. A one-sample, one-sided Wilcoxon signed-rank test indicates that the transient finite element method model achieves the prediction success criteria, DSC?≥?0.7, at a statistically significant level.     

气垫床对ICU中度压疮危险患者翻身间隔时间的影响研究

目的探讨气垫床对ICU中度压疮危险患者翻身模式的影响。方法将入住我院ICU符合条件的患者按照住院顺序随机分为A、B、C、D四组,四组患者均使用气垫床,分别每2h、3h、4h和5h翻身1次。使用简易体压测定仪测量四组患者左右肩胛部及骶尾部3个主要受压部位的皮肤表面压力值,观察受压部位在不同翻身时间间隔点的压力值及压疮发生情况。结果四组患者3个主要受压部位在不同翻身时间间隔点的体压值比较,差异无统计学意义(P0.05),四组患者在ICU住院期间压疮发生率均为0。结论气垫床ICU中度压疮危险的患者,不会增加压疮的发生,既减轻了频繁翻身给患者带来的不适感,又降低了护理人员的劳动强度,为ICU中度压疮危险患者使用气垫床时的翻身模式提供了参考。