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101.
Recent studies have shown that strychnine-insensitive glycine binding sites positively modulate the N-methyl-d-aspartate (NMDA) subclass of glutamate receptors, which are important in neural pathways involved in cognitive function. We examined the effect of (±)-3-amino-1-hydroxy-2-pyrrolidone (HA-966), a highly specific antagonist of this glycine modulatory site on the NMDA receptor, on visual recognition memory in four rhesus monkeys performing a computer-automated version of delayed nonmatching-to-sample (DNMS) with a list length of 20 trial-unique graphic symbols. In addition, the effect of HA-966 was compared with that of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine (dizocilpine; MK-801), a noncompetitive NMDA channel blocker. Administration of HA-966 (0.1–10 mg/kg, i.m.) 30 min before testing impaired DNMS performance dose-dependently, starting at doses of 3.2 mg/kg; the memory deficit following the highest dose (10 mg/kg) was associated with prolonged response latencies. Similar impairments in recognition memory were observed following treatment with MK-801, though at much lower doses (3.2–32 μg/kg) than those at which HA-966 was effective. Administration of low doses of HA-966 (1 mg/kg) and MK-801 (10 μg/kg), each of which had no significant effect on performance when given alone, also failed to impair performance when given concurrently. Combined administration of both drugs, each at amnesia-producing doses (3.2 mg/kg of HA-966 plus 32 μg/kg of MK-801), markedly impaired performance in an additive, not a synergistic, manner. From these results, we propose that the recognition memory impairment observed in our monkeys following HA-966 administration is via an action on the glycine modulatory site of the NMDA receptor complex. 相似文献
102.
Hongyu Zhang Robert J. Clarke Paul D. R. Gamlin 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1996,112(1):158-162
In cats and monkeys, extrastriate visual areas that have been reported to be involved in the near triad of pupilloconstriction, convergence, and accommodation have well-defined projections to the pretectal olivary nucleus (PON), the retinorecipient pretectal nucleus mediating the pupillary light reflex in mammals. We have therefore used alert, behaving primates to investigate the possibility that PON neurons are involved in the pupillary near response in addition to the pupillary light reflex. Single-unit recording revealed that PON luminance neurons significantly increased their firing rate with increases in retinal illumination and the resultant pupilloconstriction. In contrast, their activity did not significantly increase during pupilloconstriction elicited by near viewing. Thus the behavior of PON luminance neurons is appropriate for their participation in the pupillary light reflex, but is inappropriate for any proposed role in the pupillary near response. This result strongly suggests that neurons in the primate PON are solely related to the pupillary light reflex and that the cortical projections to this pretectal nucleus are related to this reflex and do not play a role in the pupillary near response. 相似文献
103.
Monika M. Dietl Patrick R. Hof Jean-Luc Martin Pierre J. Magistretti Jos M. Palacios 《Brain research》1990,520(1-2):14-26
The distribution of vasoactive intestinal peptide (VIP) binding sites in the brain of several vertebrate species was examined by in vitro autoradiography on slide-mounted sections. This study included fish, frog, snake, pigeon, rat, mouse, guinea pig, cat and monkey brain. A fully characterized, monoiodinated form of vasoactive intestinal peptide (M-125I-VIP), which maintains the biological activity of the native peptide in the central nervous system (CNS), was used throughout the study. Among the lower vertebrate species, no significant specific binding was found in the fish brain, whereas in the frog and snake brain, specific VIP binding sites were observed, mainly in the telencephalon. In the pigeon brain, high densities of VIP binding sites were localized in the hyperstriatum, neostriatum, archistriatum, hippocampal area, dorsolateral cortical area and in the optic tectum. Ectostriatum and paleostriatum augmentatum displayed lower densities of specific binding. In mammals, the highest concentrations of VIP binding sites were observed in the rodent brain. In the rat, mouse and guinea pig brain, high densities were detected in the olfactory bulb, external layers of the cerebral cortex, dentate gyrus, midline thalamic nuclei, geniculate nuclei, some hypothalamic nuclei, superior colliculus coeruleus. Intermediate densities were found in amygdala, caudate-putamen, septum and nucleus accumbens, CA1–CA3 fields of the hippocampus and central gray. The cerebellum of these species presented high densities of VIP binding sites, with species to species differences in their localization. The non-specific binding was, however, increased in the rodent cerebellum. Lower densities of VIP binding sites were observed in the cat and monkey CNS. In these two species, the non-specific binding was considerably higher than in the lower mammals brain. In the cat and monkey brain, as in the lower mammals, the highest densities were revealed in the neocortex, dentate gyrus, thalamic nuclei and some midbrain structures including substantia nigra and locus coeruleus. In all the species studied, the white matter was never labeled with M-125I-VIP. This study suggests that VIP binding sites appear relatively early in the evolution of the vertebrate CNS. The most important densities of specific VIP binding sites are observed in the pigeon and rodent brain, whereas the cat and monkey present a marked increase in non-specific binding. It is interesting to note that the distribution of VIP binding sites as revealed by autoradiography is quite conservative in terms of evolution and indicates an association, although non-exclusive, of VIP receptors with brain regions involved in the processing of specific sensory inputs. 相似文献
104.
Eain M. Cornford John B. Varesi Shigeyo Hyman Raymond T. Damian Michael J. Raleigh 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1997,116(3):399-405
The objective of the present study was to examine the apparent work capacity of one of the two separate membrane systems
(the blood-cerebrospinal fluid barrier) that isolate the mammalian brain extracellular fluid (and cerebrospinal fluid, CSF)
from plasma. Digitized analyses of electron-microscopic images provided estimates of mitochondrial volumes, which were expressed
as a percentage of the cell cytoplasm. We recorded a high mitochondrial content of 12–15% in the cuboidal epithelium of primate
choroid plexus, which was consistent in vervet, rhesus, and squirrel monkeys, as well as in baboons. Similarly high mitochondrial
contents were observed in the rabbit, rat, and mouse choroid plexus. It has been postulated that the high mitochondrial content
of brain endothelium is associated with maintaining the ionic gradients within the central nervous system. We observed that
the mitochondrial content of the choroid plexus (where CSF is produced) was slightly higher than in (prior measurements of)
the blood-brain barrier (BBB). In addition, surface areas at the apical borders of the choroid plexus epithelia (where the
Na+K+ATPase activity has been localized) were increased 7- to 13-fold over the basal borders, in the primate species examined.
The observation of high mitochondrial volumes in choroid plexus cells is consistent with the suggestion that increased mitochondrial
densities seen in choroidal epithelia and BBB capillaries provide a metabolic work capability for both secretory activities
and maintaining ionic gradients across blood-CSF barriers.
Received: 25 February 1997 / Accepted: 2 May 1997 相似文献
105.
106.
C. Leranth C. E. Ribak 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1991,85(1):129-136
Summary Previous immunocytochemical studies have shown a heterogeneous distribution of parvalbumin (PA) and calbindin (CB) in the rat hippocampal formation. The results of the present study showed a heterogeneous distribution of PA and CB in primate Ammon's horn. The density and intensity of immunoreactivity for both of these calcium-binding proteins was greatest in CA2 as compared to CA1 and CA3. CB-immunoreactivity was localized to the cell bodies, dendrites, and axon initial segments of pyramidal cells whereas PA-immunostaining was found in the axon terminals, dendrites and cell bodies of interneurons that have features similar to GABAergic inhibitory neurons. Based on previous studies that have shown a protective role of calcium-binding proteins in neurons exposed to hyperstimulation, these results suggest that the resistance of CA2 pyramidal cells in temporal lobe epilepsy is due to the high concentration of CB and PA in this region of Ammon's horn. 相似文献
107.
Effects of altering brain cholinergic activity on covert orienting of attention: comparison of monkey and human performance 总被引:3,自引:0,他引:3
Experiments were conducted to elucidate the role of the cholinergic neurotransmitter system in arousal and the orienting
of attention to peripheral targets. Rhesus monkeys and humans fixated a visual stimulus and responded to the onset of visual
targets presented randomly in two visual field locations. The target was preceded by a valid cue (cue and target at the same
location), an invalid cue (cue and target to opposite locations), a double cue (cues to both spatial locations, target to
one), or, the cue was omitted (no-cue, target to either location). Reaction times (RTs) to the onset of the target were recorded.
For monkeys, systemic injections of nicotine (0.003–0.012 mg/kg) or atropine (0.001–0.01 mg/kg), but not saline control injections,
reduced mean RTs for all trials, indicating general behavioral stimulation. In addition, nicotine significantly reduced RTs
for invalid trials but had little additional effect on those for valid, double, or no-cue trials. Virtually identical effects
were observed for human chronic tobacco smokers in performing the same task following cigarette smoking. Injections of atropine
in monkeys had no effect on RTs for valid or invalid trials but significantly slowed RTs in double-cue trials that did not
require the orienting of attention. These results suggest that in both species, the nicotinic cholinergic system may play
a role in automatic sensory orienting. In addition, the muscarinic system may play a role in alerting to visual stimuli in
monkeys.
Received: 8 July 1996 / Final version: 24 February 1997 相似文献
108.
A small cluster of choline acetyltransferase (ChAT)-positive neurons was identified immunohistochemically in rat and monkey brain in the ventral portion of the lateral septal nucleus. These multipolar neurons were smaller and much less intensely staining than the medial septal nucleus ChAT-immunopositive group. 相似文献
109.
目的 研究恒河猴外周血pDC1和pDC2的形态学特征。方法 采集健康、SIV阴性的恒河猴外周血,用Ficoll—Hypaque梯度离心法提取外周血单核细胞(PBMC)。利用与恒河猴有交叉反应的人单克隆抗体以及三色流式细胞仪分离pDC1和pDC2。新鲜分离和经CD40L培养的pDC1进行形态学观察。新鲜分离和经CD40L、rhIL—3和rhGM—CSF培养的pDC2进行形态学观察。结果 恒河猴外周血树突状细胞(DC)具有较大不规则偏心的细胞核,其细胞表面有大量典型的树突状突起。结论 本研究为pDC1和pDC2作为抗原递呈细胞(APC)在排斥反应中的免疫调整功能奠定基础。 相似文献
110.
J.D Elsworth M.S Brittan J.R Taylor J.R Sladek Jr. D.E Redmond Jr. R.B Innis Y Zea-Ponce R.H Roth 《Brain research》1998,795(1-2)
Although neural transplantation holds promise as a treatment for Parkinson's disease, parkinsonian primates have generally exhibited inconsistent and incomplete recovery of motor functions following intrastriatal grafting of fetal ventral mesencephalon. One possible contributing factor to this variable response is lack of appropriate integration of donor neurons with host striatal circuitry with the result that there is insufficient dopamine release and postsynaptic dopamine receptor activation. This issue was examined by measuring the effect of transplanting fetal ventral mesencephalon to the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated (MPTP) monkeys on striatal D2 receptor binding. One year after receiving MPTP, D2 receptor binding was upregulated in the dorsal and ventral striatum of African green monkeys. Grafting of fetal ventral mesencephalon to the dorsal striatum of MPTP-treated monkeys 9 months before sacrifice, eliminated the D2 receptor upregulation in dorsal, but not ventral, region. Dopamine concentration in dorsal striatum of grafted MPTP-treated monkeys was significantly higher than in that region of MPTP-treated non-grafted monkeys. In addition, dopamine concentration was significantly higher in dorsal compared to ventral striatum of grafted MPTP-treated monkeys. These data, in addition to those from a previous autoradiographic study on dopamine uptake site density in these monkeys, strongly supports the hypothesis that ectopically placed ventral mesencephalon not only produces, but maintains the release of sufficient levels of dopamine to restore postsynaptic dopamine transmission in regions influenced by graft-derived dopamine. 相似文献