近5年可穿戴技术在中医方面的应用

社会人口老龄化使传统的“以医院为中心的医疗保健”模式逐渐发生改变,中医药在慢性病诊疗方面有得天独厚的优势,但是传统的中医四诊模式、依靠医生主观经验、只能采集静态数据等束缚了中医长足发展。近年来,中医药发展已上升至国家战略,中医药产业势必要寻求新科技相结合发展。随着微纳技术、柔性电子、电子皮肤、可植入芯片、3D打印技术的不断发展和日趋完善,可穿戴技术已广泛应用于医疗健康领域,实现了远程医疗。可穿戴技术在中医诊断、疗效评价、养生、经络诊疗、治未病等方面均表现出强大的应用潜力。未来,医生和工程师通力合作,多学科交叉融合,必将推动中医药健康事业的飞速发展。本文对近5年国内外可穿戴技术在中医药领域的应用进行综述。     

浅谈慢性肾功能衰竭患者微炎症产生的机制

近年来,微炎症的概念引起了国内外众多学者的重视.越来越多的研究提示微炎症状态与心脑血管疾病、慢性肾脏病发生发展有十分密切的关系,理解微炎症产生的机制及其在疾病进展中的作用,对于防治疾病具有重要的临床意义.如何防治终末期肾病患者的微炎症状态已经成为肾脏内科医师面临的重要课题.现就慢性肾功能衰竭患者微炎症产生的机制作一阐述.     

Accurate detection of reemergent anti-donor ABO antibody is crucial for safe transfusion in ABO major incompatible HSCT patient after achieved the stages of full switch to donor type

Dynamic monitoring ABO chimera including erythroid ABO antigen and anti-A/B is crucial to not only assess the status of erythroid engraftment but also achieve personalized safety transfusion in patients post ABO incompatible hematopoietic stem cell transplantation. Transfusion support for ABO incompatible (ABOi) HSCT patients after achieved complete alteration to donor origin still remains cautious because the instant hematopoietic status on these transplant patients possibly returned to patient origin derived from early disease relapse and graft loss or failure. We reported that reemergent anti-B in a female patients (donor/patient: B/O) at the early phase after achievement complete donor type were not effectively found from partial automatic ABO blood grouping systems, which directly resulted in differential judgement of transplantation stage for about 15 days and disturbed the optimal recommendation on transfusion support. Meanwhile, the solely alteration of ABO chimera was found and earlier than changes of other markers such as MRD diagnosis, chimerism analysis by STR-PCR and sex chromosome assays, which can be an available predictors for bad transplant outcomes such as graft failure.     

Topographical variations in articular cartilage and subchondral bone of the normal rat knee are age-related

In osteoarthritis animal models the rat knee is one of the most frequently investigated joint. However, it is unknown whether topographical variations in articular cartilage and subchondral bone of the normal rat knee exist and how they are linked or influenced by growth and maturation. Detailed knowledge is needed in order to allow interpretation and facilitate comparability of published osteoarthritis studies. For the first time, the present study maps topographical variations in cartilage thickness, cartilage compressive properties and subchondral bone microarchitecture between the medial and lateral tibial compartment of normal growing rat knees (7 vs. 13 weeks). Thickness and compressive properties (aggregate modulus) of cartilage were determined and the subchondral bone was analyzed by micro-computed tomography. We found that articular cartilage thickness is initially homogenous in both compartments, but then differentiates during growth and maturation resulting in greater cartilage thickness in the medial compartment in the 13-week-old animals. Cartilage compressive properties did not vary between the two sites independently of age. In both age-groups, subchondral plate thickness as well as trabecular bone volume ratio and trabecular thickness were greater in the medial compartment. While a high porosity of subchondral bone plate with a high topographical variation (medial/lateral) could be observed in the 7-week-old animals, the porosity was reduced and was accompanied by a reversion in topographical variation when reaching maturity. Our findings highlight that there is a considerable topographical variation in articular cartilage and subchondral bone within the normal rat knee in relation to the developmental status.     

青光眼患者中MP-1微视野计与自动静态阈值视野计的对比研究

目的比较MP-1微视野计与自动静态阈值视野计（Humphrey750型）在青光眼中的应用，探讨两种检查手段的关系及二者的优缺点。方法将原发性青光眼患者56例（56眼），分别进行MP-1微视野计（意大利Nidek技术）检查及自动静态阈值视野计（Humphrey750型）检查，将检查结果分为上、下、鼻、颞、中心五个区域并相互对应，进行统计学分析。结果视野的平均光敏感度值（MS1）均比相应的微视野的平均光敏感度值（MS，）高，差值10．34±7．89dB，差异有统计学意义（P〈0．01）；二者的平均光敏感度值具有显著的直线相关性（P〈0．01）。青光眼患者均为中心注视，相对稳定者占43％，相对不稳定者占57％。在7例Humphrey750型自动静态阈值视野计检查结果正常的患者中，有6例患者MP-1微视野计检查显示盲点扩大及上方或下方弓形区敏感度下降。在4例晚期青光眼患者中，用Humphrey750型自动静态阈值视野计未检查出视野范围，而在MP-1微视野计中可查出中心管状视野和／或颞侧视岛。结论MP-1微视野计的检查结果与自动静态阈值视野计检查结果一致；其可以作为青光眼患者的检查手段，准确评价患者视功能；亦可以更早地发现青光眼病理改变，所以在青光眼的诊断及视功能评价中，它将有更广阔的应用前景。     

吡格列酮对2型糖尿病患者C反应蛋白及尿微量白蛋白作用探讨

目的：探讨吡格列酮（瑞彤）对2型糖尿病（DM）患者炎性标志物C-反应蛋白（CRP）及尿微量白蛋白（MAU）的作用.方法：测定90例血糖控制良好的2型DM患者,30例健康对照组血清CRP及MAU的量,然后对糖尿病组加用瑞彤15mg口服,每日一次,共24周.比较糖尿病组加用瑞彤前后血清CRP及MAU. 结果：糖尿病组CRP高于健康对照组（P〈 0.01）.瑞彤治疗后糖尿病组CRP及MAU均较前明显下降（P〈0.01）.结论：瑞彤能显著降低2型DM患者CRP水平及MAU,因此,瑞彤不仅能改善胰岛素抵抗作用,还能降低CRP水平及MAU,改善或减轻肾脏损害.     

不同显微测量方法的比较分析

目的:本文分析了三种显微测量方法的精度、误差、可靠性及适用性。在可靠性和综合效力方面,图像手工测量和图像分析仪具有较大优势。对于微小标本的不规则图形的长度测量,适合用图像手工测量方法。对于微小标本的规则图形或直线距离,三种方法相当,但图像分析仪和图像手工测量的精度要高一些。     

Potential of on-line micro-LC immunochemical detection in the bioanalysis of cytokines

An on-line liquid chromatography-immunochemical detection (LC-ICD) system for the quantification of cytokines in cell extracts has been developed using a post-column continuous-flow reaction detection system using fluorescence labelled antibodies. Cytokines eluting from the micro-HPLC column react with antibodies to form fluorescent complexes. In a second step the excess of free antibody is trapped on a cytokine bound support prior to fluorescence detection. The concentration detection limit of the flow injection-ICD system was 50 pM (20 μl injection volume) for interleukine 4 (IL-4). An absolute detection limit of 1 fmol was obtained for IL-4. Similar to ICD systems for small non-protein analytes developed earlier, reaction times were in the order of 1 minute. The immobilised cytokine affinity columns can easily be regenerated and used for months. The present ICD system for interleukine 4, 6, 8 and 10 was coupled to ion exchange-, size exclusion- and reversed phase chromatography. Important parameters (reaction times, reaction conditions) were investigated to get a better understanding of post-column ICD systems for macromolecules.     

大鼠坐骨神经显微解剖及其意义

研究大鼠坐骨神经显微解剖结构,探讨其对建立大鼠神经支配腓肠肌模型的意义。方法先对６０只大鼠１２０条坐骨神经进行完整显微解剖学观察,再将４８只大鼠坐骨神经在不同平面切断,建立失神经支配腓肠肌实验模型,观察肢体溃疡情况,测量肠肠肌湿重及肌细胞直径。