半导体激光光针对大鼠脑神经递质和血浆丙二醛的影响

为探讨低强度激光产生生物效应的机制，采用半导体激光器（波长６５０ｎｍ，功率１０ｍＷ，连续可调），对雄性ＳＤ大鼠和自发性高血压大鼠进行颈部“扶突”穴穴位照射，用高效液相色谱电化学检测方法测脑干和尾核中五羟色胺、去甲肾上腺素和多巴胺等单胺类神经递质含量，用ＴＢＡ荧光法测定血浆中丙二醛（ＭＤＡ），同时用氢清除法组织血流仪监测大鼠海马区的脑血流量。结果提示，光照后，海马脑血流量下降，脑干和尾核中单胺类神经递质含量明显下降，而血浆中ＭＤＡ含量略有上升。揭示激光照射“扶突”穴会引起单胺类神经递质下降。     

间歇缺氧大鼠海马神经元凋亡及其机制

目的探讨间歇缺氧对大鼠海马组织氧化应激状态及海马神经元凋亡的影响及其可能的机制。方法将36只雄性Wistar大鼠随机分为间歇缺氧组、持续缺氧组和正常对照组，每组12只。采用化学比色法测定海马组织丙二醛和超氧化物歧化酶（SOD）水平，应用Western免疫印迹法检测海马CA1区磷酸化C—JUN氨基末端激酶（p-JNK）、磷酸化c-jun（p-c-jun）的表达水平，应用缺口末端标记（TUNEL）法检测海马CA1区神经元凋亡率。结果间歇缺氧组大鼠海马CAl区丙二醛水平为（1．61±0．39）nmol／mg蛋白，显著高于正常对照组的[（1．25±0．29）nmol／mg蛋白]和持续缺氧组的[（1．34±0．24）nmol／mg蛋白]；间歇缺氧组大鼠海马CAl区SOD水平为（45±13）NU／mg蛋白，显著低于正常对照组[（58±12）NU／mg蛋白]和持续缺氧组[（56±10）NU／mg蛋白]；持续缺氧组与正常对照组的差异均无统计学意义。间歇缺氧组p-JNK、p—c-jun表达显著增高，分别是正常对照组的2．1倍及2．3倍；间歇缺氧组海马CA1区神经元凋亡率为（0．30±0．16）％，显著高于正常对照组[（0．12±0．07）％]和持续缺氧组[（0．17±0．09）]。结论间歇缺氧可导致海马CA1区氧化应激状态，从而激活JNK信号传导通路，介导海马神经元凋亡，这可能是阻塞性睡眠呼吸暂停低通气综合征患者神经功能障碍的病理生理基础之一。【     

体外四氯化碳损伤肝细胞对肝星状细胞活化的影响及其丹酚酸A的干预效果

目的 体外观察肝细胞四氯化碳过氧化损伤后肝星状细胞（ＨＳＣ）活化的影响,并观察丹参水溶性成分丹酚酸Ａ的干预效果,以研究脂质过氧化损伤与肝纤维化的关系和丹酚酸Ａ的作用机理。方法 肝细胞分离后分组,分别添加不同浓度的丹酚酸Ａ以及对照药维生素Ｅ,同时以四氯化碳熏蒸２４ｈ并测定各组细胞上清液ＡＬＴ、ＡＳＴ活性后,换液培养２４ｈ并测定ＭＤＡ含量,再以此上清液作为“肝细胞条件培养液”作用于ＨＳＣ４８ｈ,观察Ｈ     

Maternal serum AGEs levels in pregnancies associated with neural tube defects

Advanced glycation end products (AGEs) plays an important role in diabetic embryopathy. AGE-mediated DNA damage could be a significant factor in the teratogenicity. The aim of the present study was to evaluate the association between the AGEs level and neural tube defects (NTDs) occurrence risk. Forty-eight mothers with NTD-affected pregnancies and 50 normal mothers were selected in this study. Blood were collected from the mothers and were assayed for serum AGEs, malondiadehyde (MDA) and hemoglobin A1c (HbA1c). Data were analyzed by logistic regression method. The study indicated that there were significant but modest lower prevalence for cases mothers on age, BMI and glucose levels compared with controls. NTD-affected mothers were significantly more likely to have higher AGEs levels (5.6 ± 0.48 AU vs. 4.6 ± 0.68 AU ρ < 0.01) than controls. The AGEs levels were not correlated with MDA and HbA1c in NTDs mothers (r² = 0.0006 p = 0.8691 and r² = 0.001 p = 0.8172, respectively). The conclusion is that AGEs might be associated with NTDs occurrence.     

电针对血管性痴呆大鼠脑组织MDA和AchE含量的影响

[目的]探讨电针对血管性痴呆大鼠脑组织丙二醛(MDA)、乙酰胆碱脂酶(AchE)含量的影响.[方法]采用改良Pusinelli(4-VO)4-血管阻断法,建立全脑缺血再灌注大鼠痴呆模型,32只SD大鼠随机分为假手术对照组、模型对照组、电针治疗组、脑复康治疗组,给予相应措施处理后.同时处死大鼠检测其大脑皮质中MDA、AchE含量变化;用Y型电迷宫进行行为学测试.经统计学处理,比较组间差异.[结果]模型对照组大鼠Y电迷宫成绩低于假手术组(P<0.05),其大脑皮质中MDA、AchE含量测值较假手术组为高(P<0.05),各治疗组Y电迷宫学习成绩优于模型组(P<0.05);大脑皮质中MDA、AchE测值低于模型组(P<0.05);且与假手术组无显著性差异(P>0.05).[结论]电针治疗可明显改善缺血性痴呆大鼠脑组织抗氧化能力.可能与减轻脑内活性氧损伤、自由基损伤反应.从而改善其学习记忆能力而产生治疗作用.     

沙棘籽油对小鼠实验性肝硬化的保护作用及参数分析

目的：研究沙棘籽油（seed oil of hippopha erhamnoides L．）对实验性小鼠肝硬化的影响。方法：采用四氯化碳（CCl4）导致小鼠肝硬化，分别饲以不同浓度的沙棘籽油，饲养45d后分别检测小鼠血清谷丙转氨酶（GPT）和超氧化物歧化酶（SOD）及肝组织的SOD活力和丙二醛（MDA）含量的变化，观察沙棘籽油对小鼠肝的保护作用，并以GPT生化指标值为应变量，以沙棘籽油剂量为自变量，建立量效曲线。结果：沙棘籽油能明显对抗血清中GPT的升高，而且对调节SOD活力的下降及MDA的升高也有较明显效果。沙棘籽油剂量对GPT下降的函数相关性较好。结论：沙棘籽油对小鼠实验性肝硬化具有一定的保护作用。     

海带多糖对实验性大鼠动脉粥样硬化的预防作用

目的:探讨海带多糖(laminaria japonica polysaccharides,LJP)对大鼠动脉粥样硬化的预防作用。方法:SD大鼠随机分为正常对照组、模型组、阳性药对照组、LJP低剂量、中剂量和高剂量组。12周后,颈动脉取血,检测血清TG、TC、LDL-C、HDL-C及血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)水平。取血后分离主动脉,作病理形态学观察。结果:LJP能显著降低血清TG、TC、LDL-C及MDA水平,增高HDL-C水平和SOD活力,并呈剂量依赖性。主动脉HE染色病理观察显示,LJP给药组主动脉内皮脂质沉积较模型组少见。结论:LJP对高脂饲料诱导的大鼠动脉粥样硬化具有保护作用,其机制可能与其调节血脂,抗氧化作用有关。     

Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR

Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to 1 and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper.     

天麻钩藤方对肾性高血压大鼠心脏的保护作用

目的研究天麻钩藤方对肾性高血压大鼠(RHR)的心脏保护作用及其机制。方法两肾一夹法造成肾性高血压大鼠模型,随机分为天麻钩藤方高、中、低剂量组、模型组、依那普利组,另设假手术组,检测各组大鼠血压、左心室质量指数(LVMI)及心肌中羟脯氨酸(Hyp)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)的含量。结果天麻钩藤方高剂量组大鼠血压、LVMI、Hyp、MDA均明显低于模型组大鼠;SOD、GSH-PX含量明显高于模型组,差异具有统计学意义。结论该复方可能通过调节肾性高血压大鼠血压和心肌的抗氧化作用,抑制胶原增生以发挥其对心脏的保护作用。     

冠心病患者运动前后血浆丙二醛和超氧化物歧化酶含量的研究

目的:探讨冠心病患者运动前后血浆丙二醛(MDA)及超氧化物歧化酶(SOD)含量的变化和意义。方法:随机选取冠状动脉造影确诊的冠心病患者68例及对照组48例,测定2组人群运动前后血中MDA和SOD 含量及血脂并进行分析。结果:冠心病患者运动后血浆MDA含量升高而血浆SOD含量降低,对照组运动后1 h 血浆MDA和s0D含量无显著变化。冠状动脉狭窄程度越重血浆MDA含量越高而血浆SOD含量越低,差异有统计学意义。结论:运动降低冠心病患者的抗氧化能力且其抗氧化能力与冠状动脉狭窄程度成反比。