HL—60细胞在诱导分化中DNA含量及细胞动力学观察

以显微分光光度法和流式细胞计观察经维甲酸(RA)或二甲亚砜(DMSO)处理 HL-60细胞的 DNA含量和细胞增殖的动态变化.结果显示 HL-60细胞的 DNA 含量由原来高出一倍以上降至接近正常.其中 RA处理细胞 DNA 在处理后第3天和第5天有二次剧降,而 DMSO 处理细胞 DNA 量呈平缓逐日下降.两种处理细胞在诱导第2天 G_1/G_0期细胞明显增多,并在第3天达到高峰。对两种诱导剂作用机理进行了讨论.     

Antibody profiles to wheat germ cell-free system synthesized Plasmodium falciparum proteins correlate with protection from symptomatic malaria in Uganda

The key targets of protective antibodies against Plasmodium falciparum remain largely unknown. In this study, we determined immunoreactivity to 1827 recombinant proteins derived from 1565 genes representing ∼30% of the entire P. falciparum genome, for identification of novel malaria vaccine candidates. The recombinant proteins were expressed by wheat germ cell-free system, a platform that can synthesize quality plasmodial proteins that elicit biologically active antibodies in animals. Sera were obtained from indigenous residents of a malaria endemic region in Northern Uganda who were enrolled at the start of a rainy season and prospectively monitored for symptomatic malaria episodes for a year. Immunoreactivity to sera was determined by AlphaScreen; a homogeneous high-throughput system that detects protein interactions. Our analysis revealed antibody responses to 128 proteins that significantly associated with protection from symptomatic malaria. From 128 proteins, 53 were down-selected as the most plausible targets of host protective immune response by virtue of having a predicted signal peptide and/or transmembrane domain(s), or confirmed localization on the parasite surface. The 53 proteins comprised of not only previously characterized vaccine candidates but also uncharacterized proteins. Proteins involved in erythrocyte invasion; RON4, RON2 and CLAG3.1 and pre-erythrocytic proteins; SIAP-2, TRAP and CelTOS, were recommended for prioritization for further evaluation as vaccine candidates. The findings clearly demonstrate that generation of the protein library using the wheat germ cell-free system coupled with high throughput immunoscreening with AlphaScreen offers new options for rational discovery and selection of potential malaria vaccine candidates.     

Methylation of SFRPs and APC Genes in Ovarian Cancer Infected with High Risk Human Papillomavirus

Background: Secreted frizzled-related protein (SFRP) genes, new tumor suppressor genes, are negativeregulators of the Wnt pathway whose alteration is associated with various tumors. In ovarian cancer, SFRPsgenes promoter methylation can lead to gene inactivation. This study investigated mechanisms of SFRP andadenomatous polyposis coli (APC) genes silencing in ovarian cancer infected with high risk human papillomavirus.Materials and Methods: DNA was extracted from 200 formalin-fixed paraffin-embedded ovarian cancer and theirnormal adjacent tissues (NAT) and DNA methylation was detected by methylation specific PCR (MSP). High riskhuman papillomavirus (HPV) was detected by nested PCR with consensus primers to amplify a broad spectrumof HPV genotypes. Results: The percentages of SFRP and APC genes with methylation were significantly higherin ovarian cancer tissues infected with high risk HPV compared to NAT. The methylated studied genes wereassociated with suppression in their gene expression. Conclusion: This finding highlights the possible role of thehigh risk HPV virus in ovarian carcinogenesis or in facilitating cancer progression by suppression of SFRP andAPC genes via DNA methylation.     

The Plasmodium vivax homologues of merozoite surface proteins 4 and 5 from Plasmodium falciparum are expressed at different locations in the merozoite

Merozoite surface proteins of Plasmodium falciparum are one major group of antigens currently being investigated and tested as malaria vaccine candidates. Two recently described P. falciparum merozoite surface antigens, MSP4 and MSP5, are GPI-anchored proteins that each contain a single EGF-like domain and appear to have arisen by an ancient gene duplication event. The genes are found in tandem on chromosome 2 of P. falciparum and the syntenic region of the genome was identified in the rodent malarias P. chabaudi, P. yoelii and P. berghei. In these species, there is only a single gene, designated MSP4/5 encoding a single EGF-like domain similar to the EGF-like domain in both PfMSP4 and PfMSP5. Immunization of mice with PyMSP4/5 provides mice with high levels of protection against lethal challenge with blood stage P. yoelii. In this study, we show that in P. vivax, which is quite phylogenetically distant from P. falciparum, both MSP4 and MSP5 homologues can be found with their relative arrangements with respect to the surrounding genes mostly preserved. However, the gene for MSP2, found between MSP5 and adenylosuccinate lyase (ASL) in P. falciparum, is absent from P. vivax. The PvMSP4 and PvMSP5 genes have a two-exon structure and encode proteins with potential signal and GPI anchor sequences and a single EGF-like domain near the carboxyl-terminus. Rabbit antisera raised against purified recombinant proteins show that each of the antisera react with distinct proteins of 62 kDa for PvMSP4 and 86 kDa for PvMSP5 in parasite lysates. Indirect immunofluorescence assays (IFA) localized PvMSP4 over the entire surface of P. vivax merozoites, as expected, whereas, the MSP5 homologue was found to be associated with an apical organellar location consistent with micronemes or over the polar prominence.     

一种基于MSP430的便携式脑电实时采集分析系统

目的设计了一种基于MSP430的小型便携式脑电实时采集分析系统,以方便迅速地采集脑电并进行实时分析。方法根据脑电信号特性,设计了前端放大电路及滤波电路。系统以MSP430为核心,信号采集后通过PL2303进行USB传输,同时,PC端接收数据并通过软件实时处理。测试实验时,借助信号发生器产生理想信号,经A/D转换后,在MSP430控制下通过USB传输到PC端并进行实时处理。结果经多次试验,使用该系统在PC端得到较好的信号。后续对正常人在午休(浅睡眠)和清醒放松(听音乐)两种状态下进行实测及实时频域分析,得到较好结果。结论该系统便于携带,使用方便,实用性强,特别适用于高等院校实验室科学研究,应用潜力大。     

基于MSP430的液晶控制器S1D13700接口设计及应用

目的：利用液晶控制芯片S1D13700，设计一种基于MSP430单片机的液晶显示系统。方法：MSP430单片机控制总线采用模拟MSC-51系列单片机通讯方式，通过8位数据总线与S1D13700相连，地址总线分别采用间接寻址和直接寻址方式连接。结果：该设计已成功应用于心电监护仪中心电波形及相关参数的显示。结论：S1D13700的驱动程序采用C语言编程．通用性强，特别适用于便携式仪器的设计。     

基于超低功耗单片机MSP430F168的家用动态心电记录器

针对家用动态心电记录器的特点和要求,应用TI公司推出的超低功耗单片机MSP430F169为核心控制器设计了一款结构简单、功能适当、功耗低并且价格低廉适合于家庭保健用的动态心电记录器。     

肺癌患者CDH13基因启动子甲基化状态研究

目的应用甲基化特异性PCR（MSP）方法检测非小细胞肺癌（NSCLC）CDH13基因启动子甲基化情况。方法留取44例非小细胞肺癌患者手术标本及12例非肺癌患者正常肺组织,MSP分析CDH13基因启动子甲基化情况。结果 44例非小细胞肺癌中CDH13基因启动子甲基化阳性率为54.5%（24/44）,12例正常肺组织中未检测到此基因的甲基化（0/12）。CDH13基因甲基化与患者性别及吸烟与否无关;肿瘤分期越晚甲基化的发生率越高。结论原发性非小细胞肺癌中存在着较高比例的CDH13启动子甲基化,提示CDH13在非小细胞肺癌的发生中起着重要作用。     

基于Android系统的心电传输与显示系统设计

目的:设计一种基于Andriod平台的心电监护、无线传输与手机显示系统,可用于家庭或社区范围内的心电监测.方法:首先通过心电采集模块提取被监测者的心电信号,然后利用MSP430F149单片机进行A/D转换并将转换后的数据文件适当处理后经串口发送至蓝牙模块,再无线传输至智能手机,存储在手机的安全数码卡(secure digital memory card,SD)上.智能手机端通过设计蓝牙的应用程序编程接口(application programming interface,API)开发类实现与下位机通信,可打开已存储的心电数据文件并绘制成心电波形图,观察心电图是否有明显异常.结果:该系统能正常采集到人体心电信号并无线传输至手机进行显示,无线传输距离为10 m.结论:该系统的设计有利于家庭其他成员或监护人员及时了解患者的心电状况,以实现对家庭患者的健康监护.     

P16基因甲基化和P53抗体在非小细胞肺癌血清中的表达

目的 探讨P16基因甲基化和P53抗体在人非小细胞肺癌血清中的表达.方法 选择98例NSCLC患者为试验组(NSCLC组),60例健康者血清作对照(对照组),分别采用巢式甲基化特异性聚合酶链反应法及用酶联免疫吸附法(ELISA)检测其血清P16基因甲基化和P53抗体表达情况.结果 NSCLC组患者血清中P16甲基化和P53抗体与对照组分布差异有统计学意义(P<0.01);有吸烟史的P16基因甲基化表达率和P53抗体水平高于无吸烟史患者(P<0.01);病理为鳞癌的患者的P16甲基化的危险度是病理为腺癌或其他的2.724(1.087~6.826)倍(P<0.01);P16基因甲基化与P53抗体在NSCLC血清中的表达具有相关性.结论 P16基因甲基化和P53抗体的表达与NSCLC发生密切相关,且吸烟有协同作用;P16基因甲基化与P53抗体在NSCLC的表达有相关性.