Left ventricular function during systole and diastole in mitral incompetence

Left ventricular function during systole and diastole was studied in 17 young patients with mitral incompetence and sinus rhythm; 13 had established chronic mitral incompetence, 3 had prolapsing posterior leaflet syndrome and 1 had ruptured chordae tendineae. In chronic mitral incompetence and prolapsing posterior leaflet syndrome, clinical disability was related to a low forward stroke index, a large regurgitant volume and tall left atrial V wave. The defect was mechanical, and the ventricle had adapted by compensatory dilatation so that overall systolic function was normal, as measured by peak rate of rise of left ventricular pressure ($\text{dP}\text{dt}$) and ejection fraction. The dilated ventricle operated at a normal or slightly increased end-diastolic pressure, a consequence of normal resting tension (O point) and a more elastic myocardium with a low rate of change of instantaneous stiffness (m) and a flattened diastolic pressure-volume curve.In acute mitral incompetence, systolic function was normal but the sudden volume overload caused the unprepared ventricle with an almost normal modulus of elasticity and slope (m) to ascend its pressure-volume curve in diastole. End-diastolic pressure was increased.     

小蓟提取液对四种癌细胞生长抑制作用的研究

目的:研究小蓟提取液对癌细胞生长的抑制作用。方法:细胞形态观察和活细胞计数法研究小蓟提取液对人白血病细胞K562、肝癌细胞HepG2、宫颈癌细胞Hela、胃癌细胞BGC823生长的抑制作用。结果:小蓟提取液可使四种癌细胞发生皱缩、变圆、脱壁、碎裂等形态变化,生长受到明显抑制,抑制率最高可达88.27%。结论:小蓟有确切的抑癌作用,并与中医的气血理论、药物归经理论和肝肾同源理论相一致,再次说明了中医基本理论在体外细胞水平上也有良好体现,对实验室手段研究中医药同样有着重要指导意义。     

MB/BacT培养仪的临床应用评价及检测结果分析

目的:初步评价MB/BacT结核检测仪用于临床标本检测的效果,了解本地结核分枝杆菌的耐药情况.方法:通过对仪器检出报阳时间、阳性率、污染率及药敏结果的分析,了解仪器的效能及结核分枝杆菌的耐药情况.结果:检测标本821份,总阳性率18.15%,总污染率8.65%,其中痰标本总阳性率为24.72%,污染率14.51%,仪器平均报阳时间14.8天±2.5天,药敏试验36株受试菌总耐药率72.22%,其中MDR-TB 36.11%(13/36).结论:MB/BacT检测系统用于临床标本的检测具有敏感性高、快速、自动化、全封闭无交叉污染及放射性污染等优点,但应严格控制杂菌标本过度生长的干扰,并配合L-J法药敏试验和开展L型结核菌培养方面的工作,呼吁尽快建立统一的耐药监测方法.     

The Association of Inadequate and Intensive Prenatal Care With Maternal,Fetal, and Infant Outcomes: A Population-Based Study in Manitoba,Canada

ObjectiveLittle is known about how prenatal care influences health outcomes in Canada. The objective of this study was to examine the association of prenatal care utilization with maternal, fetal, and infant outcomes in Manitoba.MethodsThis retrospective cohort study conducted at the Manitoba Centre for Health Policy investigated all deliveries of singleton births from 2004-2005 to 2008-2009 (N = 67 076). The proportion of women receiving inadequate, intermediate/adequate, and intensive prenatal care was calculated. Multivariable logistic regression was used to examine the association of inadequate and intensive prenatal care with maternal and fetal-infant health outcomes, health care use, and maternal health-related behaviours.ResultsThe distribution of prenatal care utilization was 11.6% inadequate, 84.4% intermediate/adequate, and 4.0% intensive. After adjusting for sociodemographic factors and maternal health conditions, inadequate prenatal care was associated with increased odds of stillbirth, preterm birth, low birth weight, small for gestational age (SGA), admission to the NICU, postpartum depressive/anxiety disorders, and short interpregnancy interval to next birth. Women with inadequate prenatal care had reduced odds of initiating breastfeeding or having their infant immunized. Intensive prenatal care was associated with reduced odds of stillbirth, preterm birth, and low birth weight and increased odds of postpartum depressive/anxiety disorders, initiation of breastfeeding, and infant immunization.ConclusionInadequate prenatal care was associated with increased odds of several adverse pregnancy outcomes and lower likelihood of health-related behaviours, whereas intensive prenatal care was associated with reduced odds of some adverse pregnancy outcomes and higher likelihood of health-related behaviours. Ensuring women receive adequate prenatal care may improve pregnancy outcomes.     

围产期窒息后血浆cTnI、CK-MBmass的影响因素及临床应用价值

为探讨围产期窒息后血浆心肌肌钙蛋白I（cTnI）、肌酸激酶心型同工酶质量（CK-MBmass）的影响因素及临床应用价值。对71例围产期窒息新生儿及27例对照组新生儿生后6小时－48时血浆cTnI、CK-MBmass水平进行测定，运用逐步回归等方法进行分析。结果显示：（1）血浆cTnI水平的变化与胎龄、5分钟Apgar评分有关。（2）围产期窒息组足月儿（59例）cTnI明显高于对照组足月儿（19）；重度窒息组（17例）与轻度窒息组（16例）比较，cTnI差异无显著性，而CK-MBmass差异有非常显著性；围产期窒息组的重度心脏损害患儿（8例）cTnI、CK-MBmass水平明显高于无重心脏损害患儿（63例）。表明围产期窒息持续时间越长cTnI的水平越高。相对于CK-MBmass而言，cTnI对心肌损伤的敏感性可能稍差，而且由于cTnI的水平随着胎龄的增加而增加，因此这一指标在判断早产儿心肌损伤时有一定的局限性。     

Identification of a Strong Promoter of Bacteriophage MB78 that Lacks Consensus Sequence Around Minus 35 Region and Interacts with Phage Specific Factor

A strong promoter of bacteriophage MB78 does not have minus 35 consensus sequence although it has a TGn motif immediately upstream of minus 10 sequence as well as the AT rich UP element. It is efficiently recognised by the sigma 70 RNA polymerase, however, a phage-specific factor competes with sigma 70 RNA polymerase for binding to this region, the binding of the factor being stronger than that of the polymerase. Contrary to the reports in the literature the polymerase appears not to bind to the UP element whereas the phage-specific factor does. The latter seems to be involved in the regulation of the promoter activity. This revised version was published online in July 2006 with corrections to the Cover Date.     

磺化聚醚砜对光敏剂-亚甲蓝的吸附去除研究Ⅱ.磺化聚醚砜对血浆中亚甲蓝的吸附去除研究

研究了磺化聚醚砜吸附柱对血浆中亚甲蓝的吸附效果,并检测了流经吸附柱的牛血清白蛋白随时间变化的规律以及过柱前后血浆中主要生化指标的变化情况.结果:磺化聚醚砜对亚甲蓝的吸附效果明显优于聚醚砜对亚甲蓝的吸附效果;其对白蛋白的吸附较小;对血浆中主要生化指标的影响可以忽略不计.     

Sensitive and high sensitivity next generation cardiac troponin assays: more than just a name

In the 20 years that cardiac troponin testing has been available in clinical laboratories, the biomarker has revolutionised testing of patients with acute coronary syndromes. Cardiac troponin I and T testing has become the cornerstone for diagnosis of myocardial infarction and is useful for risk assessment and management of suspected acute coronary syndrome patients. As evidence and knowledge have evolved, it has become clear that even small troponin elevations are associated with adverse health outcomes. As a result there have been several generations of troponin assays, all toward tests that reliably detect lower concentrations of this critical analyte. Guidance for cardiac troponin interpretation has been in the form of myocardial infarction redefinition and evidence-based clinical and analytical guidelines. Although terminology naming generations for cardiac troponin assays has been inconsistent, state-of-the-art cardiac troponin assays are generally referred to as 'sensitive' assays and are in general compliance with analytical guidelines. Evidence shows that use of a sensitive troponin assay can result in diagnosis of myocardial infarction earlier. Next generation cardiac troponin I and T assays will likely be termed 'high sensitivity'; these assays should have the ability to measure troponin with a CV of total error of <10% at concentrations significantly lower than the 99 percentile of the normal reference population. As such, these assays should reliably measure troponin in most normal individuals and detect troponin changes (delta values) below the 99 percentile. This property may result in earlier ACS diagnosis and better management. Utilisation of high sensitivity troponin measurements may be useful for applications other than acute coronary syndromes including risk stratifying patients with renal insufficiency, heart failure, cardiac amyloid and screening elderly patients.     

Cloning,Sequencing, Expression and Promoter Analysis of a Structural Protein of Bacteriophage MB78

Bacteriophage MB78, a virulent phage of Salmonella typhimurium isolated in our laboratory. It is different from the well-known temperate phage P22 and 9NA. A detailed physical map has been constructed. To understand more about the physiology and genetics of this interesting phage it has become necessary to fragment the phage genome, clone the fragments and analyze in depth. A number of promoters of bacteriophage MB78 have been cloned and characterized recently. As a part of this program, in this investigation, we report cloning, sequencing and expression and promoter analysis of the ClaI G fragment. We identified the expressed protein as phage structural. Phage structural proteins play a vital role in forming the core head of the phage particle.