Effect of the traditional Chinese medicines Chan Su,Lu-Shen-Wan,Dan Shen,and Asian ginseng on serum digoxin measurement by Tina-quant (Roche) and Synchron LX system (Beckman) digoxin immunoassays

Chan Su, Lu-Shen-Wan, Dan Shen, and Asian ginseng are traditionally used to treat a number of conditions, including cardiovascular disease. All of these traditional Chinese medicines exhibit cardioactive properties. Digoxin is a cardioactive drug with a narrow therapeutic range (0.8-1.9 ng/mL). A patient taking digoxin may also take these Chinese medicines for their cardiotonic effects. Moreover, the active components of these medicines that are responsible for cardiotonic effects bear structural similarities to digoxin. Therefore, we studied the potential interference of these Chinese medicines with two digoxin immunoassays--the Tina-quant (Roche Diagnostics) and the Beckman (Synchron LX system)--and compared the values with the fluorescence polarization immunoassay (FPIA; Abbott Laboratories). When very small amounts (2-5 microL) of aqueous extract of Chan Su or Lu-Shen-Wan were added to drug-free serum, we observed high digoxin-like immunoreactivity with the FPIA. In contrast, when ethyl acetate extract of Dan Shen or microliter amounts of ginseng extract were added to drug-free serum, we observed modest digoxin-like immunoreactivity with the FPIA, but no apparent digoxin activity with the Roche and Beckman digoxin immunoassays. When aliquots of a digoxin pool prepared from patients receiving digoxin were supplemented with these Chinese medicines, we observed the most significant interference with the FPIA. The presence of endogenous digoxin-like immunoreactive substances can have additive effects with these Chinese medicines and falsely increase apparent digoxin levels by the FPIA. On the other hand, the Roche and Beckman assays were free from interference from DLIS but showed significant interference from Chan Su and Lu-Shen-Wan. We conclude that the FPIA showed the most significant interference from all four of the Chinese medicines we studied. However, the Roche and Beckman assays showed no interference from two (Dan Shen and Asian ginseng) of the four Chinese medicines we studied.     

多波长线性回归法同时测定安痛定注射液中的三组分

目的：对安痛定注射液中的三组分实行分光光度法同时测定。方法：对传统的多波长线性回归分光光度法进行改进,使其不需要等吸收波长。结果：９份模拟样品中氨基比林、安替比林、巴比妥的平均回收率分别为９９．９％、９９．８％、１００．３％,相对标准偏差分别为０．５％、１．０％、１．７％。对３个批号实际样品的测定结果与标准方法一致。结论：方法简便快捷,适于安痛定注射液的制剂分析。     

剖宫产术后促排气的方法和护理措施探讨

目的观察促进剖宫产术后肛门排气有效的方法。方法回顾性分析我院自2007年6月～2009年6月对84例剖宫产术后的产妇随机分三组,分别给予中药排气汤、乳果糖口服溶液、开塞露三种方法促其排气,观察比较三组产后机体恢复情况和对宫高的影响。结果三组药物治疗后腹胀均有明显缓解,术后早期排气、排尿和排便效果上,开塞露组〉乳果糖组〉中药排气汤组,经统计学检验有显著性差异（P〈0.05）。中药排气汤组对促子宫收缩的影响与乳果糖组或开塞露组经统计学检验有差异（P〈0.05）。结论中药排气汤、开塞露或乳果糖,均可减轻术后腹胀,有利于产妇身体的康复,更有利于母乳喂养。     

卢海涛内科验案3则

卢海涛老师治学严谨,锲而不舍,博极医源,注重实践,辨证精确,善治内妇科疑难杂症。耳轮灼痛案,实因阴虚火旺,风热上乘,治以滋阴降火,平肝熄风;戒烟后口腔溃疡案,实因脾胃虚弱、湿热内蕴、阴虚血燥,治以益气健脾、清化湿热、养阴凉血;尿频案,实因脾肾阳虚,固摄失权,治以湿补脾肾,缩泉固关。     

陆金根辨治慢性非特异性溃疡性结肠炎验案1则

溃疡性结肠炎（ulcerative colitis,UC）,又称慢性非特异性溃疡性结肠炎,是一种不明原因的慢性炎症性肠病,病变主要限于结肠的黏膜和黏膜下层,表现为炎症或溃疡,多累及直肠和远端结肠,但可向近端扩展,以至遍及整个结肠。其临床特点为持续性或反复性黏液血便、腹痛伴有不同程度的全身症状。     

Preparation and preliminary biological evaluation of 177Lu‐labeled GluDTPA‐cyclo(RGDfK) for integrin ανβ3 receptor‐positive tumor targeting

Integrin α_νβ₃ is a receptor and is highly expressed on activated and proliferating endothelial cells during the growth and metastasis of solid tumors but not on resting endothelial cells and normal organs. Because RGD peptide binds to integrin α_νβ₃ receptor, a variety of radiolabeled RGD peptides have been evaluated for non‐invasive imaging of integrin α_νβ₃‐positive tumors. In an attempt to develop RGD‐based radiopharmaceuticals, a novel GluDTPA‐cyclo arginine‐glycine‐aspartic acid‐d ‐phenylalanine‐lysine (GluDTPA‐cycloRGDfK) was simply synthesized and radiolabeled with ¹⁷⁷Lu. Also, tumor targeting and retention of the radiolabeled complex were evaluated in U87MG glioma‐bearing mice. The ¹⁷⁷Lu‐labeled GluDTPA‐cyclo(RGDfK) was formulated with a high radiolabeling yield (>98%) under mild condition, and the radiochemical purity was sustained in both saline and serum for over 4 days at 37°C. The radiolabeled compounds were rapidly cleared from the blood pool and non‐target tissue. Tumor‐to‐blood ratio was 12.09 at 2 h post injection and increased to 134.67 at 24 h, while tumor to liver ratio was 2.01 at 24 h similar to that of 2 h. Though it is inappropriate for targeted therapy due to its low uptake in tumor (~ 1 %ID/g), the acceptable results on radiochemistry and biodistribution propose to take a further assessment for non‐invasive imaging and detection of integrin α_νβ₃‐positive tumors by applying diagnostic radionuclides. Copyright © 2011 John Wiley & Sons, Ltd.     

张锡纯运用虫类药物的学术思想探讨

张锡纯运用虫类药物组方,广泛运用于临床各科,疗效显著.总结张锡纯根据中医辨证论治理论运用虫类药物的临床经验及对虫类药物独到的见解,对临床具有重要的指导意义.     

维脑路通治疗急性期脑出血大鼠实验研究

用维脑路通对大鼠进行急性期脑出血的治疗研究,结果表明,维脑路通对动物的肢瘫治愈率达100％,动物死亡率为5％。该药能扩张血管,促进血肿吸收,使血小板功能恢复正常。用维脑路通治疗急性期脑出血效果可靠。     

罗汉果三倍体雌株与二倍体雄株遗传背景的ISSR分析

目的探索罗汉果Siraitia grosvenorii三倍体雌株与二倍体雄株的遗传背景,为无籽罗汉果优良品种的选育提供分子生物学依据。方法利用ISSR分子标记对28份罗汉果种质进行遗传背景分析,用AFLP-SURV计算材料间遗传距离;用TSYS-pc和Gen AIEx分别进行聚类和主坐标分析。结果从100条ISSR引物中筛选出13条用于扩增,共获得131条清晰可辨条带,其中多态性条带99条,多态性位点百分率为75.57%。聚类和主坐标分析表明罗汉果三倍体雌株和二倍体雄株的遗传背景存在一定的丰富性,但大多遗传相似性系数较大,遗传距离较近。结论罗汉果三倍体雌株和二倍体雄株遗传背景的复杂性较低,应该尽快采取相应措施,进行种质创新,丰富无籽罗汉果亲本的遗传背景。     

基于中医传承辅助平台探索陆曙教授治疗冠心病用药规律

应用数据挖掘方法,探索陆曙教授治疗冠心病的用药规律。方法：收集整理门诊中药处方,应用中医传承辅助平台（V3.0）建立数据库,进行数据挖掘,分析用药频次及药物间的关联规则,以探究其用药经验。结果：本研究共纳入陆曙教授治疗冠心病的门诊病例480例,提取处方462首,统计分析出高频用药24味,前5位药物为丹参、生甘草、茯苓、砂仁、紫檀,四气五味统计发现药性以温、寒为主,药味以甘、辛为主,由此进一步推演出核心处方3首。结论：陆曙教授治疗冠心病善用经方及自创方,常用药为丹参,常用方为交泰调脉方及丹参饮,用药方面寒温并用,且用量平和。其针对肾虚、火热、气郁、痰瘀多见,善以理气解郁、活血化瘀、化痰利湿。