Linearity and dissociative antigen noise analyses of competitive microfluidic heterogeneous immunoadsorption

Presently, microfluidic heterogeneous immunoadsorption (MHI) has been regarded as an effective and efficient on-chip sample preparation method for various clinical diagnoses. In this work, adsorption performances of an electroosmosis flow (EOF)-based competitive MHI under saturated status are studied numerically. Along with the well-known flow and surface reaction control modes, the diffusion control mode specified in the fluidic configuration is scrutinized to characterize the MHI performance. The numerical results indicate that only in the surface reaction or the diffusion control mode can saturated EOF-based MHI obtain a linear dose-response along with a perfect assay signal without so-called dissociative antigen noise. Considering the tunable assay time and realizable flow velocity required by chip applications, the diffusion controlled MHI is recommended in this work.     

HPLC法测定抗癌口服液中野黄芩苷的含量

目的:建立高效液相色谱法测定抗癌口服液中野黄芩苷的含量。方法:色谱条件采用Sc ienhom e-C18柱,以甲醇-水-醋酸(35:61:4)为流动相;流速为1.0 m l.m in-1;检测波长为335nm,柱温:29℃。结果:野黄芩苷在0.2603μg~0.7810μg范围内呈良好线性关系;其回归方程为A=0.933X+1.92(r=0.9997);平均回收率为98.01%;RSD为2.14%(n=6)。结论:本方法操作简单、准确,可用于测定抗癌口服液中野黄芩苷的含量。