层粘连蛋白时间分辨免疫分析方法的建立

采用时间分辨荧光免疫分析(TRFIA)技术建立高灵敏的层粘连蛋白(LN)的快速的全自动检测方法.以羊抗鼠IgG抗体包被板,采用抗人LN单克隆抗体(McAb)、Eu3 标记人LN和以β-二酮体为主的增强液建立竞争LN-TRFIA,数据采用双对数函数处理程序处理.结果表明LN的标记率为11.5 Eu3 /LN,方法的批内和批间CV分别为3.9%和6.7%,平均回收率为91.2%,灵敏度为5μg/L,可测范围为5～1000μg/L,ED20、ED50和ED80分别为723.1μg/L、183.4μg/L和56.95μg/L.临床结果与RIA结果相符.本文建立的LN-TRFIA灵敏、稳定,可全自动操作,有很好的应用前景.     

B cell elimination in systemic lupus erythematosus

Systemic lupus erythematosus (SLE) is an autoimmune disorder with a worldwide distribution, potentially life-threatening with considerable morbidity. The elimination of pathogenic B cells has emerged as a rational therapeutic option. Many open label studies have reported encouraging results in which clinical and serological remission have invariably been described, often enabling the reduction of steroid and immunosuppressive treatment. However, the results from randomized controlled studies have been disappointing and several questions remain to be answered. In this review we will focus on results of B cell direct depletion in the treatment of patients with systemic lupus erythematosus.     

整合素-β1在层黏连蛋白促进施万细胞合成细胞外基质过程中的作用

目的 探讨整合素-β1在层黏连蛋白(LN)促进施万细胞(SCs)合成细胞外基质(ECM)过程中的作用.方法 体外培养10只SD大鼠来源的SCs,经外源性LN 处理后,双重免疫荧光细胞化学法检测LN和整合素-β1的表达情况,免疫印迹法检测SCs内黏着斑激酶(FAK)的磷酸化水平.用抗体Ha2/5封闭细胞表面受体整合素-β...     

精液中HA、LN、PCⅢ、CⅣ含量与精子质量关系的研究

目的:探讨精液中透明质酸(HA),层粘连蛋白(LN),Ⅲ型前胶原(PCⅢ),IV型胶原(CⅣ)与男性精子质量的关系。方法:采用放射免疫分析法分别检测60例男性生育者和不育者精液中的HA、LN、PCⅢ、CⅣ,并进行精子质量分析。结果:精液液化时间正常与异常组精液中HA、PCⅢ、CⅣ及LN含量差异无统计学意义;精子密度正常组精液中HA、PCⅢ及LN含量明显高于异常组,两组比较有统计学意义(P<0.01),而两组中CⅣ含量比较差异无统计学意义(P>0.05);精子活动率异常组精液中HA和LN含量明显高于正常组,两组比较差异有统计学意义(P<0.01),而两组中的PCⅢ和CⅣ含量比较差异无统计学意义(P>0.05)。结论:精液中HA、LN的升高可能是生精功能活跃的表现,且不排除HA、LN是刺激男性生精因素的可能,测定精液中HA、LN的含量可以间接了解生育能力。     

排毒合剂对慢性肾衰竭纤维化指标和肾功能影响的临床研究

目的:观察排毒合剂对慢性肾衰竭(CRF)患者血清纤维化指标、肾功能的作用。方法:将50例惠者随机分为2组,同时给予基础治疗,治疗组30例配合排毒合剂(组成:生地黄、山茱萸、熟大黄、黄芪、山药、牡丹皮、茯苓皮、桃仁、白术、丹参、当归、鱼腥草、车前子)治疗;对照组20例配合药用炭片治疗。观察肾功能指标血清肌酐(Cr)、尿素氮(BUN)和纤维化指标血清人层粘连蛋白(LN)、血清Ⅳ型胶原(CL-Ⅳ)的影响。结果:显效率及总有效率2组比较,差异均有显著性意义(P<0.05)。2组治疗后肾功能指标变化比较,差异有非常显著性意义(P<0.01)。2组治疗后纤维化指标比较,差异有非常显著性意义(P<0.01)。治疗组均明显优于对照组。结论:排毒合剂可明显改善CRF的临床症状及实验指标,其作用可能与该药能抑制肾纤维化有关。     

The presence of laminin in the fetal human inner ear

Summary The expression of laminin was analyzed in the human fetal inner ear using immunohistochemical methods. In the 11-week-old human fetus, the presence of laminin was found in the basement membrane of the immature cochlea, endolymphatic sac and vestibular end organs. The reaction of the basement membrane of the endolymphatic sac was strong in the 15-week-old human fetus. A laminin reaction was seen in the cochlea, Reissner's membrane, epithelial cells of the limbus spiralis, the basilar membrane and the stria vascularis. In particular, the capillaries and basement membrane of the stria vascularis were strongly positive. These results suggest that laminin may be an essential component in the development of the inner ear and may possibly be related to filtration of the endolymph.     

恶性肿瘤患者血清层粘蛋白含量的初步研究

利用酶联免疫吸咐法（ＥＬＩＳＡ）检测了１３５例恶性肿瘤患者清中层粘蛋白的含量。比较了良恶性肿瘤及健康成人血清ＬＮ的差别，对ＬＮ在癌变中的机制进行了初步探讨。结果表明，所有恶性肿瘤患者清ＬＮ值均高于健康对照（Ｐ＜０．００１），并且高于良性肿瘤患者（Ｐ＜０．００１），良性肿瘤和健康组之间没有差别（Ｐ＞０．０５）。以正常人ＬＮ值上限为界，恶性肿瘤阳性分布率为７４．８％，良性肿瘤１３％，正常人假阳性为８．     

Expression of Laminin in Hepatocellular Carcinoma: An Adjunct for Its Histological Diagnosis

In general, hepatocytes lack basement membrane structures andtherefore no laminin expression is seen around hepatic cords.To determine whether or not laminin expression appears whenhepatic tissue becomes carcinomatous, we carried out immunohistochemicalstaining of hepatic tissues excised surgically from 35 patientswith hepatocellular carcinoma, 18 with metastatic colon carcinoma,two with adenomatous hyperplasia, and 10 without any nodularlesions. Among the various conditions of hepatic tissue, lamininexpression was detected only in hepatocellular carcinoma with86% positivity. The result was not dependent on the degree ofdifferentiation. Therefore, it was confirmed that immunohistochemicaldetection of laminin provides a useful adjunct for the diagnosisof hepatocellular carcinoma, and this was verified by a studyusing needle biopsy samples. In addition, our results suggestedthat the basement membranes are derived from endothelial cellsof either portal veins or hepatic arteries.     

福辛普利对肾小球系膜细胞层粘连蛋白及mRNA表达的影响

目的 观察新一代血管紧张素转化酶抑制剂（ACEI）福辛普利（FOS）对体外培养的大鼠肾小球系膜细胞（GMC）产生的细胞外基质（ECM）成分层粘连蛋白（LN）的影响及FOS防治肾小球硬化的机制。方法 按经典方法体外培养大鼠GMC，转种培养后分为对照组、脂多糖（LPS）组和LPS＋FOs组。采用ELISA法测定GMC培养上清液LN含量，荧光半定量PCR方法检测LNmRNA的表达。结果 LPS组GMC分泌LN蛋白及mRNA的表达明显高于对照组（P〈0．05），LPS＋FOS组GMC分泌LN蛋白及mRNA的表达较LPS组明显下降（P〈0．05）。结论 FOS从蛋白和mRNA两个水平对体外培养大鼠GMC产生的LN有明显的抑制作用，提示FOS抑制GMC产生ECM可能是延缓肾小球硬化的重要作用机制之一。     

层粘连蛋白LN及其受体LN-R在髓母细胞瘤中的表达

目的测定Laminin(LN)／Laminin Receptor(LN-R)在髓母细胞瘤中的表达情况，进一步探讨其与该肿瘤浸润、转移的关系。方法通过免疫组化方法测定LN和LN-R在70例髓母细胞瘤和10例正常小脑组织中的表达情况。结果LN-R在10例正常小脑组织及70例髓母细胞瘤中表达均为阴性，LN在髓母细胞瘤中低表达。结论LN及其受体在髓母细胞瘤中低表达，其中LN-R不表达可能为原发性中枢神经系统肿瘤不易发生颅外转移的原因之一。