导管法测定大鼠肺循环血流量的研究

目的：研究一种新的导管法测定肺循环血流量。方法：分离右侧颈外静脉,将塑料导管自右侧颈外静脉插入,经上腔静脉、右心房和右心室插入肺动脉。用上海产ＳＪ—４２型四导生理记录仪记录生理参数。结果：肺循环血流量可按公式ＱＰ＝ＭＰＡＰ·△ＱＭＰＡＰ－ＭＰＡＰ计算,式中△Ｑ是每分钟自肺循环流入容量测定计的血量,算得的ＱＰ＝２９．６２±３．７３ｍｌ／ｍｉｎ,在大鼠的肺循环血流量正常范围之内。结论：该方法精确而且操作简便     

A method for the follow-up of clusters of adverse reproductive outcomes

Clusters of adverse reproductive outcomes are reported with increasing frequency to public health authorities. Most are random events and only a very small proportion is likely due to identifiable environmental agents. Often, a preliminary study confirms the existence of a spatial and temporal excess but no biologically plausible cause is found. These cases require a follow-up of incidence to identify any continuing excess. A conceptually simple fixed window technique of follow up is proposed. The power of the test is mainly influenced by the magnitude of the increase in rate, the number of adverse outcomes selected for observation and the acceptable false alarm rate. This technique has several advantages compared with other currently used methods. From a public health point of view, two important factors are to be considered in setting the parameters of the test: the delay in providing an answer for the community and the availability of resources for conducting environmental investigations.     

Holladay法计算准分子激光角膜切削术屈光矫正效应的应用

目的　评价计算准分子激光角膜切削术（ＰＲＫ） 矫正近视性屈光不正的角膜屈光力变化量的Ｈｏｌｌａｄｙ 法。 方法　用Ｈｏｌｌａｄａｙ 法计算ＰＲＫ 术后与术前角膜屈光力的差值（ 角膜实矫屈光度） ,并与角膜预矫屈光度进行比较。 结果　（１）Ａ 组：复性近视散光眼术后６ 个月,角膜实矫球镜度欠矫（０ ．４８ ±０ ．１９）Ｄ;角膜实矫散光度过矫３８ ．５ ％ （２５／６５） ,散光欠矫６１ ．５ ％ （４０／６５） ,过矫和欠矫≥±０ ．５０ Ｄ 占４１ ．５ ％ （２７／６５） ;实矫与预矫散光的轴位的差值≤±１５°占６０ ．０ ％ （３９／６５） 。（２）Ｂ 组：单纯近视眼术后６ 个月时,角膜实矫等值球镜度欠矫（０ ．５５ ±０ ．２６） Ｄ;术后６ 个月时产生（０ ．５９ ±０ ．３８）Ｄ 的矫正近视散光的效应,其中≥０ ．５０ Ｄ 者占５１ ．２ ％ （２２／４３） ,实矫近视散光轴位均在１８０°－ ４４°～１８０°＋ ４４°之间。 结论　应用Ｈｏｌｌａｄａｙ 公式从球、柱镜度两方面计算ＰＲＫ 的角膜屈光变化效应值,是合理、客观和准确的     

角膜异物分区分级和记录方法

目的探讨角膜异物分区、分级及记录方法。方法对５６０例角膜异物分区、分级研究,在此基础上提出一种较为适用的记录方法,并用此指导临床实践。结果角膜异物有其分布特点,区、级不同,治疗及愈后不同。用此记录方法指导临床实践准确方便,不会出现误治漏治。结论角膜异物分区、分级及记录方法在临床上科学实用,在法医学上亦有使用价值     

Human pharmacokinetics of an analogue of atrial natriuretic factor

Summary The pharmacokinetics of ANP-270, a 26 amino acid analogue of alpha human natriuretic factor (-hANF) with a prolonged effect on isolated arterial preparations has been studied in 40 healthy males, in a doubleblind placebo controlled investigation. Placebo or ANP-270 0.3, 1.5 or 3.0 g/kg were given by intravenous bolus injection, each to groups of 10 subjects. Blood samples were assayed for ANP-270 by a specific sandwich ELISA.The disappearance of ANP-270 from plasma followed a two-compartment decay, with mean distribution and elimination half-lives of 2.6 min (n = 30) and 10.6 min (n=20), respectively. These estimates were similar to those obtained by other investigators for -hANF. Their brevity explains the lack of a prolonged effect of ANP-270 in vivo compared to -hANF.     

激光治疗闭角型青光眼

目的：评价激光虹膜切除治疗闭角型青光眼临床疗效。方法：应用Nd：YAG激光于上方或下方作周边虹膜切除。结果：107例122眼闭角型青光眼术后经过2—106月随访观察，成功115眼，失败7眼。结论：激光虹膜切除术治疗闭角型青光眼安全、有效。     

口腔愈疡颗粒质量标准的建立

目的：建立口腔愈疡颗粒的质量标准。方法：采用高效液相色谱法对成品中芍药苷进行含量测定。结果：芍药香浓度线性范围为：0．104—0．520μg/ml,γ=0.9993。样品平均回收率为98．85％，RSD为1．27％(n＝5)。结论：该方法适用于口腔愈疡颗粒的质量控制。     

Estimation of the amniotic fluid volume using the Cavalieri method on ultrasound images.

OBJECTIVES: Presently, a design-based and practical method for measuring amniotic fluid volume (AFV) for routine clinical examinations has not been proposed. In this study we describe a new method, which combines the Cavalieri method with ultrasound imaging to estimate AFV. METHODS: We measured the AFVs of 14 women in the third trimester of pregnancy, and repeated our measurements three times for each woman. Parallel planimetric ultrasonographic images were obtained at every 2 cm along the longitudinal uterine axis. AFVs were calculated as the total of the multiples of the estimated cut surface areas by the section thickness. RESULTS: The mean estimated AFV was 380.5 cm3. The coefficient of error of each measurement was calculated and the mean was 0.108. The coefficient of correlation between the amniotic fluid index and our AFV estimations was 0.87. CONCLUSIONS: Design-based and efficient estimation of AFV is possible with the combination of consecutive ultrasound images and the Cavalieri method.     

离子抑制色谱法测定琥乙红霉素含量及其有关物质

目的　建立离子抑制色谱法测定琥乙红霉素原料的含量及有关物质的方法 ,并研究其影响因素。方法　采用ZorbaxSB -C1 8色谱柱 ,以 0 .0 2mol·L- 1 KH2 PO4 乙腈 ( 4 5∶5 5 ,用氨试液调至pH 6 .8)为流动相 ,流速 1.2mL·min- 1 ,检测波长 2 10nm ,柱温 ( 30± 0 .5 )℃。结果　在选定固定相条件下 ,流动相对组分的洗脱和选择性影响最大 ,柱温次之 ,琥乙红霉素在乙腈中比较稳定。测定了有效主成分琥乙红霉素A的含量以及 7个有关物质的总含量 ,琥乙红霉素A的平均回收率为 99.4 5 %(RSD =2 .2 4 % ,n =5 )。结论　本方法可用于琥乙红霉素原料的含量测定和有关物质的检查。