气相色谱法测定土木香药材中土木香内酯和异土木香内酯的含量

土木香来源于菊科植物,中华人民共和国药典1985年版(一部)规定其来源为土木香Inula helenium L.和总状土木香I.racemosaHook f.的根。前者主产于河北省安国县,当地称之为青木香、祁木香。后者常为藏民所     

Allergic contact dermatitis to methylenelactones use of lymphocyte transformation test

Summary Seventeen guinea pigs were sensitized to alantolactone, a natural sesquiterpene lactone known for its sensitizing properties, using intradermal injections in Freund's complete adjuvant. Guinea pig skin protein extracts (SPE) were used to make conjugates with alantolactone and an isomer, isoalantolactone. Lymphocyte blastogenesis was observed with SPE-alantolactone conjugates, as well as with guinea pig albumin-lactone conjugates. In six cases, stimulation was noted with unconjugated haptens. These results do not show a high degree of hapten-carrier specificity in contact sensitivity to alantolactone, induced by unconjugated hapten injections. Cross-sensitivity with SPE-isoalantolactone conjugate was also observed.     

果旭阿汤中土木香内酯和异土木香内酯的含量测定

［摘要］目的建立同时测定藏药果旭阿汤中土木香内酯和异土木香内酯含量的方法。方法通过优化提取方法,用气相色谱（GC）法同时测定藏药果旭阿汤中藏木香有效成分土木香内酯和异土木香内酯的含量。结果土木香内酯和异土木香内酯均在0.1～1.0 mg•mL-1 (n=5）范围内呈良好线性关系。土木香内酯回收率为97.25% (n=6),RSD=0.93%;异土木香内酯回收率为97.42% (n=6), RSD=0.90%。结论该方法可用于藏药果旭阿汤中土木香内酯和异土木香内酯的含量同时快速、准确测定。