基于SPD的检验试剂二级库管理系统设计探讨

目的:优化检验试剂的采购流程,达到检验试剂的精细化管理、降低医院检验试剂的库存成本。方法:在医院现有的供应链管理管理系统的下面增加检验试剂二级库管理模块,再与医院LIS系统的收费端口对接,精确测算项目收入。结果:所有检验试剂的信息可以溯源。检验科内部以设备为中心,分组管理。所有检验项目的收费和试剂成本均可准确统计,帮助绩效分析。结论:检验试剂的二级库精细化管理,可以提高检验试剂的管理效率、保证检验项目合同的准确执行,还可以降低医院的库存成本。     

上海市闵行区公立医院供应链一体化应用探索

目的分析上海市闵行区公立医院供应链一体化应用的成果。方法选择上海市闵行区一家三乙公立医院,比较供应链一体化建设前后的管理数据;同时采用焦点组访谈,分析面临的主要问题。结果该医院通过建设医用耗材和试剂的供应链一体化服务模式,显著减少医务人员的采购、申领业务量;由公司统一提供专业化运输与配送;实现批号/有效期绑定,识别近效期品,按照先进先出原则进行出库,并全程追踪;实行定数管理,保证适量的库存量,减轻库房空间和资金负担。结论该供应链一体化服务模式可满足公立医院的需求。     

人类基因检测相关试剂评价国家参考品研制技术要求介绍

目的：进一步规范人类基因检测相关试剂评价国家参考品的研制工作。方法：人类基因检测相关试剂评价国家参考品是指用于人类基因检测相关试剂质量评价的参考物质。本文详细介绍了这类参考物质的原材料筛选、制备、标定、稳定性研究、包装、储存及供应方面的要求。结果与结论：人类基因检测相关试剂评价国家参考品研制过程更加清晰,管理更加规范。     

不同试剂检测D-3-羟丁酸的性能比较分析

目的比较不同试剂检测D-3-羟丁酸的性能。方法选择一种进口试剂和二种国产试剂检测8一羟丁酸,对其精密性、准确性、线性范围、相关性及抗干扰能力进行比较。结果三种试剂具有良好的相关性、精密度及抗干扰能力;线性Randox试剂达4．0mmol·L^-1,国产A试剂为3．39mmol·L^-1,国产B试剂为3．26mmol·L^-1。结论三种试剂均能满足临床实验要求,国产试剂因价格优势而具有良好应用前景。     

探讨医用耗材及试剂集中网上采购管理

本文探讨了医用耗材及试剂的网上采购管理情况、存在的问题及改进建议。     

PACK-CXL: corneal collagen cross-linking for infectious keratitis北大核心CSCD

Infectious keratitis is one of the leading causes of irreversible vision loss. With the emergence of multidrug-resistant pathogen, despite appropriate diagnosis and antimicrobial treatment, infectious keratitis can still result in corneal opacity and even melting and perforation. Therefore, it is imperative to develop novel treatment for infectious keratitis. More recently, several articles have demonstrated the effectiveness of collagen cross-linking (CXL) in treating infectious keratitis via direct antimicrobial activity and inhibition of corneal enzymatic degradation. In order to highlight the use of CXL for infectious keratitis treatment, a new term was proposed at the 9th CXL congress to rename its use in infections as photoactivated chromophore for infectious keratitis-CXL (PACK-CXL). Based on the current literature, PACK-CXL appears to be effective for bacterial keratitis, especially on inhibiting corneal perforation. The effect was limited for fungal and amoebic keratitis, and maybe aggravative on viral keratitis. In this article, we will review the basic profile, technical operation, prevention of complication of CXL, the principle mechanism of PACK-CXL and its clinical application for bacterial, fungal keratitis, amoebic and viral keratitis. Copyright © 2018 by the Chinese Medical Association.     

有关鲎试剂的研究进展

鲎试验是近 10多年来研究建立的革兰氏阴性菌内毒素检查法。本文从鲎试剂检测内毒素的原理、鲎试剂的制备与质量控制、鲎试验的种类与应用等方面对有关鲎试剂的研究进展进行了综述 ,并对今后的研究趋向提出了自己的几点看法。     

分离兼屏蔽计数技术在放射免疫分析中应用

本文应用活性炭与氧化铋相结合,制备了一种新的放射免疫分析(RIA)分离兼屏蔽试剂,应用于胆酸、T4的RIA中。该方法与聚乙二醇(PEG)分离方法比较,其标准曲线相关系数(r)分别为0.997和0.990,差别无显著意义(P>0.5)。本方法限用小于分子抗原,非特异结合较高,有待进一步改进。     

Development of a rapid assay methodology for antimicrobial susceptibility testing of Staphylococcus aureus

Development of a new phenotypic technique for rapid antimicrobial susceptibility testing (AST) of methicillin-resistant Staphylococcus aureus is presented. The new technique combines bacterial culturing and specific immunometric detection in a single separation-free process. The technique uses dry chemistry reagents and the recently developed two-photon excitation detection technology, which allows online detection of bacterium-specific growth. The performance of the new technique was evaluated by monitoring the growth of S. aureus reference strains and determining their susceptibility to oxacillin. In the direct analysis of clinical specimens, method specificity and tolerance to interferences caused by other bacteria present in the sample are pivotal. Other bacteria can compete with the bacteria of interest for nutrients, for example. Specificity and tolerance were studied against Staphylococcus epidermidis reference strains. The results suggest that the new technique could allow rapid AST directly from clinical samples within 6 to 8 h. Such a rapid and simple testing methodology would be a valuable tool in clinical microbiology because it would shorten the turnaround times of microbiologic analyses. Advantages of the new approach in relation to conventional methods are discussed.     

Animal models of spinal injury for studying back pain and SCI

IntroductionBack pain is a common ailment affecting individuals around the globe. Animal models to understand the back pain mechanism, treatment modalities, and spinal cord injury are widely researched topics worldwide. Despite the presence of several animal models on disc degeneration and Spinal Cord Injury, there is a lack of a comprehensive review.Material and methodA methodological narrative literature review was carried out for the study. A total of 1273 publications were found, out of which 763 were related to spine surgery in animals. The literature with full-text availability was selected for the review. Scale for the Assessment of Narrative Review Articles (SANRA) guidelines was used to assess the studies. Only English language publications were included which were listed on PubMed. A total of 113 studies were shortlisted (1976–2019) after internal validation scoring.ResultThe animal models for spine surgery ranged from rodents to primates. These are used to study the mechanisms of back pain as well as spinal cord injuries. The models could either be created surgically or through various means like use of electric cautery, chemicals or trauma. Genetic spine models have also been documented in which the injuries are created by genetic alterations and knock outs. Though the dorsal approach is the most common, the literature also mentions the anterior and lateral approach for spine surgery animal experiments.ConclusionThere are no single perfect animal models to represent and study human models. The selection is based on the application and the methodology. Careful selection is needed to give optimum and appropriate results.