全文获取类型
收费全文 | 207篇 |
免费 | 9篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 1篇 |
妇产科学 | 1篇 |
基础医学 | 50篇 |
口腔科学 | 2篇 |
临床医学 | 19篇 |
内科学 | 34篇 |
皮肤病学 | 2篇 |
神经病学 | 6篇 |
特种医学 | 1篇 |
外科学 | 5篇 |
综合类 | 32篇 |
预防医学 | 55篇 |
眼科学 | 1篇 |
药学 | 4篇 |
中国医学 | 3篇 |
肿瘤学 | 1篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 4篇 |
2015年 | 7篇 |
2014年 | 8篇 |
2013年 | 9篇 |
2012年 | 9篇 |
2011年 | 12篇 |
2010年 | 12篇 |
2009年 | 7篇 |
2008年 | 6篇 |
2007年 | 7篇 |
2006年 | 7篇 |
2005年 | 6篇 |
2004年 | 3篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 12篇 |
2000年 | 10篇 |
1999年 | 8篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 6篇 |
1993年 | 1篇 |
1992年 | 6篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有217条查询结果,搜索用时 15 毫秒
31.
32.
选择HFRS姬鼠型疫区盘锦大洼县、家鼠型疫区葫芦岛市,每个疫区选一试区与对照区,于每一试区分别接种HFRS长爪沙鼠肾细胞(Ⅰ型)和地鼠肾细胞(Ⅱ型)灭活疫苗各5000人份。经过一个流行周年观察,显示两种疫苗安全,近期效果明显。沙鼠苗的中强反应率为2.13%(51/356),荧光抗体阳转率免前为6.2%(隐性感染),3针基础免疫后为100%,一年后为54.76%,加强针后为100%;抗体GMT滴度分别为:31.7、798、16、557;中和抗体4次阳转率分别为0,70.37%、0、73.33%,抗体GMT滴度分别为0、10.7、0、14.6。地鼠苗的中强反应率为3.97%(51/1284),荧光抗体4次阳转率分别为9.88%(隐性感染)、100%、36.36%、100%;抗体GMT滴度分别为33.6、232、25、387;中和抗体4次阳转率分别为0、50%、17.65%、100%,抗体GMT滴度分别为0、9.3、20、15。但疫苗免疫针次、抗体持续时间、疫苗的远期流行病学效果等,有待进一步研究。 相似文献
33.
34.
Tourdias T Hiba B Raffard G Biran M Nishiguchi T Aussudre J Franconi JM Brochet B Petry KG Dousset V 《Experimental neurology》2011,230(2):248-257
We aimed to determine an optimal protocol for inducing a focal inflammatory lesion within the rat brain that could be large enough for an easier MRI monitoring while still relevant as a multiple sclerosis (MS) like lesion. We adapted a two-hit model based on pre-sensitization of the Lewis rat with myelin oligodendrocyte protein (MOG) followed by stereotaxic injection of pro-inflammatory cytokines (TNFα + IFNγ) within the internal capsule. We compared the following two strategies to increase focal lesion development for an easier MR translation: (1) a higher sensitization step (MOG50) or (2) a higher cytokine step with lower sensitization (MOG25). Control animals were administered only cytokines without MOG pre-sensitization. Animals were followed with T2, diffusion and T1 post gadolinium weighted images at 1, 3 and 7 days following cytokine injection. Immunostaining was performed at the same time points for macrophages (ED1), myelin (MBP and Luxol Fast Blue) and blood brain barrier integrity (IgG). At day 1, the focal lesions depicted with T2-weighted images were very similar among groups and related to vasogenic edema (high apparent diffusion coefficient (ADC), gadolinium enhancement and IgG extravasation) induced by cytokines irrespective of the pre-sensitization step. Then, at day 3, MOG50 rats developed statistically larger T2 lesions than MOG25 and control rats that were correlated with inflammatory cell accumulation. At day 7, MOG50 rats also showed larger T2 lesions than MOG25 and control rats, together with loss of anisotropy that were correlated with demyelination. In contrast, MOG25 and control rats developed similar MR lesions decreasing over time and almost undetectable at day 7. We conclude that with a high pre-sensitization step, the focal lesion can be monitored by MRI whose signal reflects some features of a MS-like lesion, i.e. edema, inflammatory cell accumulation and later demyelination. 相似文献
35.
Chih-Hsueh Yang Duen-Huey Tan Wei-Li Hsu Ting-Ting Jong Chi-Luan Wen Shih-Lan Hsu Poa-Chun Chang 《Journal of ethnopharmacology》2014
Ethnopharmacological relevance
Peperomia sui Lin and Lu (Peperomia sui), a well-known Taiwanese folk medicine, has a broad range of biological effects, especially in treatment of upper respiratory tract diseases. However, no previous study has explored the activity of Peperomia sui against influenza virus infections. This study was carried out to evaluate the anti-influenza virus activity and the potential virucidal effect of the ethanolic extract of Peperomia sui (PSE).Methods
The anti-H6N1 avian influenza viral activity of PSE against the influenza virus A/Chicken/TW/0518/2011 (H6N1) in chicken fibroblast DF-1 cells was evaluated by cell viability assay, hemagglutination assay, neuraminidase activity assay, indirect immunofluorescence assay and quantitative RT-PCR assay.Results
PSE significantly increased the viability of cells that were infected by the H6N1 virus. PSE also suppressed the synthesis of viral nucleoprotein (NP), and inhibited the growth of the virus in DF-1 cells. Further, PSE inhibited the neuraminidase activity of H6N1 virus.Conclusions
The findings of this study provide important information for the exploitation and utilization of Peperomia sui in treatment of influenza infection. 相似文献36.
Larsen M Jensen KB Christensen PA Suarez E Paris D Sanz L Ravn P Sauce D Saas P Goletz S Alvarez-Vallina L Kristensen P 《Journal of immunological methods》2008,339(2):220-227
Antibodies capable of recognizing key molecular targets isolated e.g. by phage display technology have been used in the pursuit of new and improved therapies for prevalent human diseases. These approaches often take advantage of non-immunogenic antibody fragments to achieve specific toxin-, radioactivity- or effector-domain delivery. There is now a growing interest in using anti-idiotypic antibodies or other antigen mimics to induce potent immune responses against antigen structures in question. We have earlier reported on the functional rescue of antibodies that are active when fused to the phage, but inactive as soluble protein [Jensen, K.B., Larsen, M., Pedersen, J.S., Christensen, P.A., Alvarez-Vallina, L., Goletz, S., Clark, B.F. and Kristensen, P. (2002) Functional improvement of antibody fragments using a novel phage coat protein III fusion system. Biochem. Biophys. Res. Commun. 298, 566-73.]. The rescue was accomplished by maintaining the fusion between the antibody fragment and portions of the filamentous bacteriophage coat protein 3, as present in the original antibody-displaying phage. In the present study, we have applied this system in an attempt to improve immunogenicity of anti-idiotypic antibodies isolated by phage display. Here we demonstrate that by preserving linkage between phage antibody and the N-terminal domain of phage coat protein 3, we induce multimerization of the antibody fragments, and improve their immunogenicity. This immunization approach allows induction of anti-idiotypic antibodies in mice, and facilitates the use of antibodies that are non-functional as non-fused soluble protein. 相似文献
37.
Expression of the betapapillomavirus (betaPV) E6/E7 genes has been shown to impair both keratinocyte differentiation and apoptosis. Especially late-terminal keratinocyte differentiation shares certain aspects with apoptosis, such as fragmentation of DNA and activation of caspases. Here we investigated the disruption of keratinocyte differentiation in organotypic skin (raft) cultures of primary (PHK) and immortalized (N/TERT) human keratinocytes, in particular by human papillomavirus (HPV)8.Immunohistochemical analysis of HPV5 and HPV8 E6/E7-expressing PHK revealed thickening of the rafts and complete absence of stratum corneum formation, even after 18 days of culture. This phenotype was confirmed in N/TERT raft cultures. When expressed separately, the aberrant morphology was observed only in rafts expressing E6, not E7. Immunofluorescence analysis of HPV8 E6 PHK rafts showed an increase in number and size of Filaggrin- and Caspase-14-positive cells in the granular layer. In raft lysates analyzed by western-blot, the presence of pro-Caspase-14 in the differentiated keratinocytes was confirmed, but in the HPV8 E6 rafts none of the Caspase-14 subunits were detected.In conclusion, in the raft system, HPV8 E6 prevented late-terminal keratinocyte differentiation resulting in an accumulation of Filaggrin and pro-Caspase-14-positive cells in the absence of stratification. This differentiation arrest was accompanied by the failure to express Caspase-14 subunits, suggesting absence of Caspase-14 activation and probable abrogation of Filaggrin maturation in HPV8 E6-expressing keratinocytes. 相似文献
38.
目的建立检测血清中猴泡沫病毒(SFV)抗体的间接免疫荧光方法,为检测实验用猴群中SFV的感染情况提供参考依据。方法用SFV-1病毒感染BHK-21细胞,待50%细胞出现病变时,用胰蛋白酶消化细胞后以2×107/mL浓度40μL的细胞滴到10孔镀膜的玻片上,丙酮固定。利用制备的抗原片通过间接免疫荧光法对34份猴血清标本进行检测。结果建立了检测SFV抗体的间接免疫荧光染色方法,SFV抗体阳性19例,15例血清检测为阴性。结论本方法具有良好的特异性,可作为SFV检测的可靠方法。 相似文献
39.
Myint KS Gibbons RV Iverson J Shrestha SK Pavlin JA Mongkolsirichaikul D Kosoy MY 《Transactions of the Royal Society of Tropical Medicine and Hygiene》2011,105(12):740-742
The Bartonella-associated illnesses are spread world-wide and involve a broad spectrum of signs and symptoms in humans. Several Bartonella species have been shown to be responsible for cases of febrile illnesses. Little information exists on distribution of Bartonella species and their role in human diseases in Nepal. Our preliminary study, a retrospective serological survey of archived specimens, suggests that Bartonella antibodies are prevalent among febrile patients in the Kathmandu Valley of Nepal. 相似文献
40.
目的探讨多重实时荧光定量PCR(MRT-PCR)检测9种常见呼吸道病原体的临床应用价值。方法采用Primer Express3.0(ABI)和Primer3 Input 4.0设计引物和探针建立MRT-PCR法,通过构建质粒标准品分析该方法的最低检出限和特异性。并对204例临床标本中副流感病毒1、2、3型、肺炎支原体、肺炎衣原体、呼吸道合胞病毒、腺病毒、甲型流感病毒、乙型流感病毒进行回顾性检测。结果 MRT-PCR法检测病原体的最低检出限达103copies/m L,特异性达100%,无交叉反应。204例咽拭子标本中,MRT-PCR检出呼吸道合胞病毒23例,副流感病毒1、2、3型13例,腺病毒15例,肺炎支原体15例,肺炎衣原体3例,甲型流感病毒13例与乙型流感病毒6例。该结果与其他试剂报告结果完全一致。结论多重实时荧光定量PCR具有快速、准确、特异性强等特点,在呼吸道病原体检测方面有重要价值。 相似文献