血液透析患者甲状旁腺功能亢进术后1年各相关血液指标达标情况的调查分析

目的：探讨血液透析患者甲状旁腺功能亢进术后1年内各相关血液指标的达标情况,为深入的研究提供一定的依据。方法本院于2010年10月~2013年10月对收治的56例血液透析患者甲状旁腺功能亢进术后1年内的血红蛋白、钙磷代谢、白蛋白、血脂、甲状旁腺激素等指标进行检测并研究,观察患者各项指标的达标情况。结果血红蛋白达标36例,总达标率为64.3%;钙磷代谢达标30例,总达标率为53.6%;白蛋白达标26例,总达标率为46.4%;血脂达标30例,总达标率为53.6%;甲状旁腺激素达标36例,总达标率为64.3%。结论分析血液透析患者甲状旁腺功能亢进术后1年内的血红蛋白、钙磷代谢、白蛋白、血脂、甲状旁腺激素等指标的达标情况可以很好地了解患者的身体恢复情况,从而制订更合理的治疗方案。     

Dominant β-Thalassemia with Hemoglobin Hradec Kralove: Enhanced Hemolysis in the Spleen

We describe a 6-year-old girl and her mother with dominant beta-thalassemia due to hemoglobin Hradec Kralove (Hb HK). Both patients presented microcytic anemia, jaundice, splenomegaly, cholelithiasis, and recurrent hemolytic bouts. Osmotic resistance tests using saline and coiled planet centrifugation revealed the increased fragility of the red cell membrane. On the other hand, the glycerol lysing time was prolonged, and results of the isopropanol test were weakly positive. Despite mimicking the features of hereditary spherocytosis, the results of the genetic analyses verified the second reported family with Hb HK (codon 115, GCC [Ala] --> GAC [Asp]). Splenectomy was effective for the amelioration of hemolysis. Of 7 reported patients with Hb variants at beta-globin codon 115 (Hb Madrid and Hb HK), 5 underwent splenectomy. Because of the variable augmentation of extramedullary hemolysis in dominant beta-thalassemias, genotyping is necessary for determining the clinical indication of splenectomy.     

Stable Acetaldehyde Adducts: Structural Characterization of Acetaldehyde Adducts of Human Hemoglobin N-Terminal β-Globin Chain Peptides

Acetaldehyde is the first oxidation product of ethanol in vivo. Our earlier work showed that with sufficient acetaldehyde, five of the six possible sites of the peptide pentalysine were moddied as a Schiff base (Braun KP, et al: J Biol Chem 270:11263–11266, 1995). However, we were unable to deduce unequivocally which site was unmodified. Lysine residues, as well as the amine terminal valine residues, in hemoglobin have been implicated as target structures for acetaldehyde adducts resulting from ethanol consumption. Hemoglobin adducts of acetaldehyde have been used clinically as a marker of ethanol consumption, but the chemical nature of these adducts remains undefined. As part of our continuing structural characterization of acetaldehyde-protein adduct formation, we studied the peptides Val-His-Leu-Thr-Pro and Val-His-Leu-Thr-Pro-Val-Glu-Lys, from the amine terminus of the β-globin chain of hemoglobin, in vitro. Both peptides have at least one potential site for adduct formation. In the octapeptide, the N-terminal amine group of Val as well as the e-amine group of the lysine sidechain can potentially be modified by acetaldehyde. We used mass spectrometry, carbon-13 nuclear magnetic resonance, and Raman spectroscopy and characterized stable Schiff base acetaldehyde adducts of these two peptides at both reactive sites. The identification of stable Schiff base adducts with the N-terminal peptides of the β-chain of hemoglobin as well as with β-amino groups of lysine provides another possible means of monitoring ethanol consumption. The functional implications of these stable Schfl bases remains undefined.     

Elevated lipoprotein (a) levels are associated with the presence and severity of coronary artery disease in patients with type 2 diabetes mellitus

Background and aims

The role of lipoprotein (a) [Lp(a)] in coronary artery diseases (CAD) with special clinical background such as type 2 diabetes mellitus (T2DM) has not been fully determined. The aim of the present study was to investigate the relation of Lp(a) to type 2 diabetic patients with or without CAD.

Production of Antibodies That Recognize the Heterogeneity of Immunoreactive Sites in Human Hemoglobin Chemically Modified by Acetaldehyde

Human hemoglobin (Hgb) was incubated with acetaldehyde under two different conditions: (a) in the presence of 250 mM acetaldehyde for 1 hr then reduced with 100 mM NaCNBH₃ for an additional 4 hr at room temperature; and (b) in the presence of 500 mM acetaldehyde for 10 days at room temperature and then reduced with 1 mM NaBH₄ for 1 hr. It was found that 44% and 27% of free amino groups in Hgb-acetaldehyde adduct (AA) remained unmodified when Hgb was treated under conditions (a) and (b), respectively. SDS-PAGE analysis revealed that the molecular weight of Hgb-AA(a) [Hgb modified under condition (a)] was slightly greater than that of unmodified Hgb and extensive protein cross-linking had occurred in Hgb-AA(b) [Hgb modified under condition (b)]. Electrophoresis on agarose gel showed the order of negative charge was Hgb-AA(b) > Hgb-AA(a) > unmodified Hgb. Polyclonal antibody raised in rabbits using keyhole limpet hemocyanin as the carrier protein modified by acetaldehyde under condition (a) [i.e., KLH-AA(a)] preferentially recognized Hgb-AA(a), whereas antibody raised using KLH-AA(b) as the immunogen recognized only Hgb-AA(b). In conclusion, antibodies raised with protein-AA antigens produced under different conditions recognize different epitopes.     

Lack of knowledge of glycosylated hemoglobin in patients with diabetic retinopathy

We surveyed 220 patients with diabetic retinopathy attending a tertiary eye hospital in Australia, and found that knowledge of HbA_1c, and the proportion achieving target level of <7%, remained low in patients with diabetic retinopathy. These data re-emphasize the gap between clinical guidelines and actual management of patients with diabetes.     

高原藏区健康人群血红蛋白值检测结果分析

目的了解高原藏区健康人群血红蛋白值增高的原因。方法随机选择甘孜藏区道孚县950名健康成年男性和760名健康成年女性作血红蛋白值检测,并与平原对照标准作比较。结果高原藏区人群血红蛋白水平高于平原地区(P0.01),该地区男性血红蛋白水平高于女性(t=26.133,P0.01)。其男女性各年龄段健康人群血红蛋白值差别无统计学意义(P0.05)。结论高原藏区健康男性血红蛋白值较女性明显增高,除与高海拔缺氧相关外,与男性不良生活习惯有一定关系。     

肠内与肠外两种营养支持疗法在重症监护病房危重症患者综合治疗中的应用比较

目的 比较肠内与肠外两种营养支持疗法在重症监护病房（ICU）危重症患者综合治疗中的疗效.方法 选择ICU危重症患者114例,按随机数字表法分为肠内营养组（57例）和肠外营养组（57例）,治疗2周后比较两组血红蛋白、总蛋白、白蛋白、前清蛋白等营养指标及并发症发生情况.结果 两组患者治疗2周后各营养指标均有所升高,且肠内营养组比肠外营养组升高更明显[血红蛋白：（120.47±22.46） g/L比（114.83±23.86） g/L,总蛋白：（78.21±8.42） g/L比（70.48±8.21） g/L,白蛋白：（38.21±5.03） g/L比（33.87±5.62） g/L,前清蛋白：（245.57±44.61） mg/L比（182.24±42.73） mg/L],差异有统计学意义（P＜0.05）.肠内营养组腹胀腹泻、呕吐恶心、感染、肝肾功能损伤发生率均明显低于肠外营养组[14.04％（8/57）比26.32％ （15/57）,12.28％（7/57）比17.54％（10/57）,3.51％（2/57）比8.77％（5/57）,1.75％（1/57）比5.26％ （3/57）],差异均有统计学意义（P＜0.05）.结论 营养支持对于危重症患者的营养状况和机体免疫力都有提升作用,且肠内营养效果更加明显,可更好地提高预后,可能成为危重症患者更佳的营养支持疗法.     

Associations of the antioxidant capacity and hemoglobin levels with functional physical performance of the upper and lower body limbs

GeroScience - Herein we considered the role of oxidative stress on deficiencies of functional physical performance that could affect a future pre-frailty condition. Using principal component...