不同温度加热自体骨成骨活性的对比实验研究

目的研究加热对自体骨成骨活性的影响.方法通过手术造成家兔胫骨1cm的骨缺损,原位分别植入新鲜自体骨、加热100℃20min及50℃20min自体骨,于手术后2、4、8、12周进行X线摄片,并对平片的相对灰度进行分析,测定骨痂胶原蛋白的含量,并与组织学检查进行对比.结果新鲜自体骨成骨活性最高,其次是加热50℃20min自体骨,加热100℃20min自体骨成骨活性最差.结论加热影响植入骨的成骨活性,而较低温度加热可以保留一定的成骨活性.     

自体牙移植术的临床效果

目的 评价自体牙移植的临床效果.方法 搜集近10年来的38例(42颗牙)自体牙移植病例,在术后1、3年进行随访观察,从治疗方法、牙根发育情况、牙髓治疗时机、牙根吸收情况及牙齿的稳固性等多方面进行分析总结.结果 1年后成功37颗牙(88.10%),尚可接受5颗牙(11.90%).3年后随访到32例36颗移植牙,成功27颗牙(75.00%),尚可接受7颗牙(19.44%),失败2颗牙(5.56%).结论 自体牙移植术是一种较为成功的治疗方法,能达到快速纠正个别牙错位以及修复一些缺失牙的目的 .     

颅神经嵴干细胞成牙本质样细胞分化诱导的体内实验研究

目的观察诱导颅神经嵴干细胞(cranial neural crest stem cell,CNCSC)后的裸鼠体内成牙本质样细胞表型分化,探讨CNCSC用于牙再生研究的可行性。方法颅神经管组织块无血清条件培养法获取CNCSC,体外经成纤维生长因子8(FGF8)、骨形态发生蛋白2(BMP2)、转化生长因子β1(TGFβ1)、牙本质基质非胶原蛋白等因子诱导后,与胶原-壳聚糖三维凝胶支架复合植入裸鼠背部皮下,免疫组织化学方法检测型胶原表达、牙本质涎磷蛋白(DSPP)表达,鉴定其成牙本质样细胞表型分化特征。结果诱导后CNCSC植入体内后1月表现为型胶原及DSPP阳性表达,2月后DSPP表达增强,局部区域可见极性栅栏状排列的细胞分布,并可见明显的细胞外基质形成。结论CNCSC在体内可向成牙本质样细胞表型分化,为进一步开展牙再生研究奠定了基础。     

恒牙胚缺失动物模型的建立及相应乳牙牙根的吸收

目的:通过建立恒牙胚缺失动物模型,为在恒牙胚缺失情况下研究乳牙牙根的吸收奠定实验基础.方法:选择11周龄雄性Beagle犬作为实验动物,通过手术摘除继承恒的方法建立恒牙胚缺失动物模型.定期拍摄根尖片观察相应乳牙牙根的吸收情况,与乳牙生理性根吸收进行比较.当摘除恒牙胚的相应乳牙牙根出现吸收时,取颌骨标本,进行组织学观察.结果:恒牙胚存在情况下,乳磨牙牙根吸收起始于20周龄;恒牙胚摘除的乳牙牙根吸收明显延缓,起始于26～27周龄.恒牙胚缺失情况下乳牙牙根吸收的组织学表现为大量多核巨细胞主要分布于吸收牙根的牙髓侧,牙周膜侧仅有少量多核巨细胞分布.结论:成功地建立了恒牙胚缺失动物模型,模拟了恒牙胚先天缺失情况,恒牙胚缺失的乳牙牙根吸收明显晚于恒牙胚存在的乳牙.     

硅酸锂全瓷高嵌体与金属高嵌体修复后牙牙体缺损的疗效比较

目的 比较硅酸锂全瓷高嵌体与金属高嵌体修复后牙牙体缺损的临床疗效.方法 搜集2015年1月至12月在安徽医科大学附属口腔医院诊治的牙体缺损患者68例,75颗患牙均采用高嵌体修复,其中49颗患牙采用硅酸锂全瓷高嵌体修复(硅酸锂组),26颗患牙采用金属高嵌体修复(金属组).修复1年后随访检查牙龈健康状况、修复体完整度、继发龋、固位、边缘适合度、邻面的接触点.结果 两组患者牙龈健康状况、修复体完整度、继发龋、固位、基牙松动、边缘适合度的差异无统计学意义(P>0.05),但在邻面接触点指标上,硅酸锂组成功率仅为77.55%,金属组成功率为96.15%,两组差异有统计学意义(P=0.036).结论 硅酸锂全瓷高嵌体是一种可行的修复方式,但是邻面接触点的恢复与保持较金属高嵌体稍差.     

Effectiveness of Reinduction and/or Dose Escalation of Ustekinumab in Crohn’s Disease: A Systematic Review and Meta-analysis

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Pathways and Fate of Migratory Cells During Late Tooth Organogenesis

Tissue recombination experiments and cell lineage analyses of the developing neural crest have documented the role and central pathways of migratory cells during early craniofacial development. In the present study, regional pathways of cells during late peripheral morphogenesis were investigated using the crown stage tooth organ as a model. Homing targets during tooth integument formation were analyzed to understand the fate of migratory cells involved in late tooth organogenesis and the developmental origin of periodontal tissues. After surgical removal of the oral mucosa, the oral aspect of the dental follicle of lower first mouse molar teeth was labeled using a fluorescent contact dye. Following sacrifice after 0, 2, 4, and 6 days, labeled cells were detected in the dental follicle, in the alveolar bone, and in the periodontal ligament adjacent to the molar root. The distribution of labeled tissues was reconstructed three-dimensionally via confocal microscopy. Using a tooth molar organ culture system, labeled cells within the dental follicle were documented traveling in the apical direction. Our results indicated that cell migration during tooth organogenesis was following specific pathways and that cells within the circumference of the dental follicle were migrating in the apical direction. We speculate that migratory cells passing through the dental follicle connective tissue may contribute to the formation of the periodontium. The present documentation visualizes pathways, role, and dynamics of extensive cell movements during late tooth organogenesis.     

Intentional partial pulpotomy to talon cusp for tooth crown morphology correction in orthodontic treatment

This report describes a case of talon cusp treated by an intentional partial pulpotomy, which made it possible to recontour the tooth crown into the desired shape. The patient was an 11-year-old boy with a large talon cusp on the lingual surface of the right maxillary lateral incisor. He was referred to our clinic for treatment of abnormality cusp because it was expected to impede orthodontic treatment. The talon cusp was amputated using an intentional partial pulpotomy. The procedure resulted in successful recontouring of the tooth crown into the desired morphology and the dental pulp exhibited a vital reaction at three years after the operation.