芒果甙对实验性肝损伤大鼠酶及形态变化影响的研究

利用三种实验模型研究了芒果甙的肝保护作用。三种大鼠肝损伤模型分别由醋氨酚(A-PAP),四氯化碳(CCI4)和D-氨基半乳糖(D-GaIN)诱导形成。芒果甙能明显降低三种肝损伤模型大鼠血清的谷丙转氨酶(GPT)和谷草转氨酶(GOT)水平,病理检查结果显示．芒果甙并能使大鼠肝损伤显著减轻,表明其具有一定的肝保护作用。     

Guillain-Barré syndrome and serum activities of gamma-Glutamyltransferase and glutamic-pyruvic transaminase

Summary Objective laboratory indicators of alcohol consumption (mean corpuscular volume and serum glutamic-pyruvic transaminase (GPT), glutamyltransferase (-GT), and glutamic-oxaloacetic transaminase (GOT)) were measured in 18 patients with Guillain-Barré syndrome (GBS) and 710 control patients. All of the indicators examined were more frequently found to be pathological in GBS patients, reaching significance for -GT and GPT. Some explanations for this result are discussed. It is concluded that alcohol consumption may be a risk factor for GBS.     

Diets containing Sophora japonica L. prevent weight gain in high-fat diet-induced obese mice

Obesity, a worldwide epidemic, is associated with metabolic diseases such as insulin resistance, dyslipidemia, hypertension, and heart disease. Many strategies, including natural alternative antiobesity agents, have been widely used to prevent obesity. Polyphenolic compounds and flavonoids from natural products are shown to inhibit adipogenesis. Because mature fruits of Sophora japonica L. were previously shown to contain antiadipogenic compounds, we hypothesized that diets with mature fruits of S japonica L. would prevent body weight gain in high-fat diet–induced obesity. Four-week-old mice were fed either a control high-fat diet, or high-fat diet containing 1% or 5% of S japonica L. for 4 weeks. The administration of S japonica L. fed in combination with a 30% high-fat diet significantly decreased body weight gain. S japonica L. also reduced serum and hepatic triglyceride, serum total, and high-density lipoprotein cholesterol. Consistent with the effects of lowering glucose level and fat mass, S japonica L. caused a decrease in the number of large adipocytes and a concomitant increase in the number of small adipocytes, which may explain at least in part the antiobesity effects of S japonica L. Together, these data provide evidence for roles of S japonica L. in the control of body weight and obesity-related metabolic diseases.     

白芍总甙对小鼠四氯化碳肝损伤模型的保护作用

白芍总甙(TGPs 40或80mg/kg·d~(-1)×3d,ip)可明显对抗四氯化碳(ig or sc)引起的小鼠血浆或血清谷丙转氨酶和血浆乳酸脱氢酶升高,但对谷草转氨酶的升高无明显影响。TGPs(40mg/kg·d~(-1)×3d,ip)对四氯化碳所致的肝病理组织改变有一定保护作用。提示TGPs可能具有保肝作用。     

Histamine H3-receptor stimulation is unable to modulate noradrenaline release by the isolated rat heart during ischaemia-reperfusion

The aim of this study was to evaluate the ability of H3-histaminergic prejunctional receptors to modulate the noradrenaline release induced by myocardial ischaemia in the rat, and the effects of an eventual modulation on haemodynamic, biochemical and electrophysiological parameters. Isolated rat hearts were perfused according to the Langendorff technique. Control hearts (n = 13) were not treated; two groups were treated with the H3-agonist R-alpha-methyl-histamine at 0.3 microM (n = 14) and 1 microM (n = 11) and one group, used as positive control, was treated with the selective alpha 2-agonist Mivazerol at 0.5 microM (n = 14) added to the perfusion medium. Noradrenaline, lactate and transaminase output in the coronary effluent, as well as various haemodynamic and electrophysiological parameters, were measured during global and total ischaemia (30 min) and reperfusion (30 min). alpha 2-receptor stimulation increased ischaemia-induced noradrenaline release during reperfusion (195 +/- 13 vs. 145 +/- 12 pmol.g-1 in control group, P < 0.05). In contrast, R-alpha-methyl-histamine, at both doses, did not significantly modify these parameters. Both treatments did not affect ischaemia- and reperfusion-induced haemodynamic (decrease in heart rate or in left ventricular developed pressure), biochemical (lactate and GOT release) and electrophysiological (arrhythmias or increase in action potential duration) alterations. Unlike other species, the rat appears to be insensitive to H3-histaminergic receptor modulation of ischaemia-induced noradrenaline release, although a modulation can be seen with other prejunctional receptor agonists.     

新生儿黄疸血清总胆汁酸测定的临床意义

对 43例新生儿黄疸用自动生化分析仪测定血清总胆汁酸 (TBA) ,并与GPT、GOT测定对照。结果TBA测定阳性率为 6 9.77% ,而GPT、GOT测定阳性率分别为 16 .9%和 44 .6 % ,TBA明显高于GPT、GOT(P <0 .0 1)。对TBA测定在新生儿黄疸治疗中的意义进行了讨论     

Unterschiede im Verhalten glucoplastischer Aminosäuren-Enzyme in der Rattenleber

Zusammenfassung Es wurde die zeitlich unterschiedliche Induzierbarkeit der Aminotransferasen (GOT, GPT) und der Serin-Dehydratase durch Hunger in der Rattenleber untersucht. Dabei wurde die Alanin-Ketoglutarat-Aminotransferase erst etwa 10 Std nach der Aspartat-Ketoglutarat: Aminotransferase und Serin-Dehydratase induziert. Beide Aminotransferasen zeigten nach 7tägiger eiweißfreier Diät cinen signifikanten Aktivitätsanstieg, der 24 Std danach wieder zurückging. Bei beiden Aminotransferasen ließ sich trotz Glucosezufuhr noch eine Hungerinduktion feststellen, während die Sexin-Dehydratase unter den gleichen Bedingungen völlig reprimiert wurde.     

Effect of Different Glycerol Levels on the Release of Glutamic Oxaloacetic Transaminase (GOT) from Deep Frozen Ram Spermatozoa

Release of GOT was determined during different treatments in ram semen extended (1:10) with lactose-egg yolk diluter containing 3.5% and 5% glycerol (v/v). Semen samples were collected by the electro-ejaculation technique and GOT was analysed colorimetrically. Highly significant difference (p less than 0.01) was found in the post-thaw percent motile spermatozoa and GOT release (IU/10(9) spermatozoa) between 3.5% and 5% glycerol containing equilibrated samples. The difference in percent motile spermatozoa was not significant between 3.5% and 5% glycerol levels in control, post-thaw unequilibrated and prefreeze equilibrated samples. Whereas the difference in GOT release between 3.5% and 5% glycerol concentrations was not significant in control and post-thaw unequilibrated samples and highly significant (p less than 0.01) in prefreeze equilibrated samples.     

Enzyme Activity of Human Ejaculates, Relation with Abnormal Liquefaction

Five different enzyme activities were estimated in ejaculates obtained from 96 men visiting our infertility clinic. Sperm count showed a significant positive correlation with aspartate-aminotransferase (GOT) and alanine-aminotransferase (GPT). Acid phosphatase was positively correlated with gamma-glutamyl transpeptidase (GGTP) and citrate and negatively with fructose. GGTP showed similar relationships with citrate and fructose. For beta-glucuronidase a low positive correlation with GGTP and GOT was detected. The enzyme activities of 27 ejaculates with a high viscosity were not significantly different from the activities of ejaculates with normal liquefaction. The conclusion is reached that insufficient prostatic enzyme secretion is not the cause of abnormal liquefaction in these patients.