The two component adjuvant IC31® potentiates the protective immunity induced by a dengue 2 recombinant fusion protein in mice

Here we evaluated the suitability of the synthetic adjuvant IC31^® to potentiate the protective capacity of PD5 protein (domain III of the envelope protein of dengue 2 virus fused to the carrier protein P64k). Unlike Alum, PD5 mixed with IC31^® induced complete protection against virus challenge in mice and increased IFN-γ secretion after in vitro re-stimulation. The induced antibody response was highly specific to the homologous serotype and showed both IgG1 and IgG2a subtypes. IC31^® is a promising adjuvant for PD5 recombinant protein based vaccination against dengue. Future work should address the suitability of PD5/IC31^® formulations in non-human primate models.     

乳腺癌术后辅助化疗开始时间与预后相关性研究

目的研究乳腺癌术后开始化疗的时间与预后的相关性,探索更适当的开始辅助化疗时机。方法回顾性分析接受辅助化疗的303例I-III期乳腺癌患者,分析其无病生存期（DFS）和总生存期（OS）。以手术后开始第1个周期化疗时间为起点时间,按起点时间不同方法分析,阶段时间为界：1～14d化疗组（1组）;15～21d化疗组（2组）;22～28d化疗组（3组）;29～35d化疗组（4组）;≥36d化疗组（5组）。结果无病生存期,在各组中无病生存期整体比较有差别（P=0.024）。各组的5年无病生存期分别为76.0%,85.2%,86.7%,63.8%,64.9%。总生存期整体比较无统计学意义。独立的预后因素中,年龄、肿瘤的大小、腋淋巴结转移数目、化疗药物四个自变量与无病生存相关;X2=11.316,P=0.023。结论乳腺癌术后需要接受术后辅助化疗的患者,术后在≤28d开始辅助化疗有益于无病生存期延长。术后辅助化疗最佳区间22～28d。其中年龄、肿瘤的大小、腋淋巴结转移数目、化疗药物四个自变量与病生存相关。     

Management of undifferentiated thyroid cancer

Management of thyroid carcinoma relies upon the tumour cells maintaining the differentiated functions that are typical of normal thyroid follicular cells, such as: dependence upon thyrotropin for growth, production of thyroglobulin and effective transport of iodine. Likewise, differentiated thyroid carcinomas often exhibit an auspicious clinical behaviour with a slow rate of growth and low potential for invasion and distant metastasis. These features permit therapy of disseminated tumour, effective follow-up surveillance and the assumption of a good prognosis. As each of these features are lost, the opportunities for both disease status assessment and therapeutic intervention diminish accordingly. A major obstacle is our failure to define effective systemic treatments to replace radioiodine therapy, whose loss is consonant with the loss of iodine transport and retention. The extreme of undifferentiated clinical behaviour is epitomized by anaplastic thyroid carcinoma, a rare, terminally dedifferentiated malignancy that is rapidly and invariably fatal. It is important to be attuned to clinical clues suggesting the presence of dedifferentiated tumour and related prognostic signs. This allows the application of currently limited therapeutic options and defines the need for research to develop new systemic treatments.     

适形放疗联合同期化疗对食管癌患者生存质量的影响

目的 观察适形放疗联合同期化疗治疗的食管鳞癌患者的生存质量趋势.方法 选用EORTC QLQ-C30量表和食管癌专用量表EORTC QLQ-OES24对60例食管癌患者进行生存质量的评价,分别在患者治疗前、治疗中40 Gy时、治疗结束以及治疗后3,6,12月时进行问卷调查.结果 同步放化疗对患者的躯体功能、社会功能以及...     

Increased anxiety-like behaviors in rats experiencing chronic inflammatory pain

For many patients, chronic pain is often accompanied, and sometimes amplified, by co-morbidities such as anxiety and depression. Although it represents important challenges, the establishment of appropriate preclinical behavioral models contributes to drug development for treating chronic inflammatory pain and associated psychopathologies. In this study, we investigated whether rats experiencing persistent inflammatory pain induced by intraplantar injection of complete Freund's adjuvant (CFA) developed anxiety-like behaviors, and whether clinically used analgesic and anxiolytic drugs were able to reverse CFA-induced anxiety-related phenotypes. These behaviors were evaluated over 28 days in both CFA- and saline-treated groups with a variety of behavioral tests. CFA-induced mechanical allodynia resulted in increased anxiety-like behaviors as evidenced by: (1) a significant decrease in percentage of time spent and number of entries in open arms of the elevated-plus maze (EPM), (2) a decrease in number of central squares visited in the open field (OF), and (3) a reduction in active social interactions in the social interaction test (SI). The number of entries in closed arms in the EPM and the distance traveled in the OF used as indicators of locomotor performance did not differ between treatments. Our results also reveal that in CFA-treated rats, acute administration of morphine (3 mg/kg, s.c.) abolished tactile allodynia and anxiety-like behaviors, whereas acute administration of diazepam (1 mg/kg, s.c) solely reversed anxiety-like behaviors. Therefore, pharmacological treatment of anxiety-like behaviors induced by chronic inflammatory pain can be objectively evaluated using multiple behavioral tests. Such a model could help identify/validate alternative potential targets that influence pain and cognitive dimensions of anxiety.     

进展期胃癌新辅助化疗前后IL-6、IL-10的变化及其临床意义

目的 观察进展期胃癌患者新辅助化疗前后白细胞介素6(IL-6)、白细胞介素10(IL-10)水平的变化,寻求新辅助化疗有效性的新的参考指标.方法 通过ELISA方法对51例进展期胃癌患者行新辅助化疗前后的IL-6、IL-10水平进行检测,分析IL-6、IL-10水平的变化与新辅助化疗有效性是否相关.根据新辅助化疗是否有效将患者分为A组(有效组:完全缓解+部分缓解),B组(无效组:疾病稳定+疾病进展).随后应用免疫组化方法,检测IL-6在新辅助化疗前后胃癌组织中的表达情况.结果 A组患者IL-6水平显著下降(P<0.05),IL-10水平有所下降但无统计学意义(P>0.05);B组患者IL-6水平有所下降,但无统计学意义(P>0.05),IL-10水平无明显变化.结论 IL-6可能作为判断新辅助化疗有效性的新的参考指标.     

Occurrence of complete heart block associated with acute renal infarction

Complete heart block (CHB) and acute renal infarction (ARI) are both uncommon diseases and seldom encountered in the clinical practice. We describe a rare case of pre‐existing left bundle branch block, presenting simultaneously with CHB and ARI. The possible mechanism depends on prior presence of either CHB or ARI. If ARI occurs first, severe pain and embolism may enhance the vagal tone resulting in decrease in the heart rate and transient intraventricular conduction interruption, which subsequently causes CHB. The opposite scenario, CHB preceding ARI, is also possible. CHB can be physiologic and transient, with higher risk of development in the circumstance of pre‐existing conduction system disturbances. Patients with CHB are predisposed to formation of thrombi and thromboemboli, giving rise to ARI. In conclusion, awareness and timely identification of the clinical manifestations of these two diseases may facilitate early diagnosis and prompt management.