S波段高功率微波辐射对原代培养乳鼠心肌细胞的影响

目的研究S波段高功率微波（HPM）辐射后乳鼠心肌细胞的损伤效应及其机制。方法采用平均功率密度为5～30mW/cm^2的S波段HPM模拟源辐射原代培养的乳鼠心肌细胞，应用流式细胞术、原子力显微镜观察辐射后心肌细胞凋亡和坏死率、[Ca^2＋]。及细胞膜形态。结果10—30mW／cm^2的S波段HPM辐射后6h，心肌细胞凋亡和坏死率增加（P〈0．05或P〈0．01），且随辐射剂量增大，坏死细胞增多；30mW/cm^2 HPM辐射后即刻[Ca^2＋]i升高（P〈0．01），细胞膜发生穿孔。结论10—30mW/cm^2的S波段HPM辐射可造成心肌细胞凋亡和坏死，[Ca^2＋]；升高以及细胞膜穿孔，是S波段HPM辐射致心肌细胞损伤的重要机制。     

烧伤后淋巴细胞膜流动性变化

报告以ＤＰＨ作为膜标记探剂，用荧光偏振法测定烧伤后淋巴细胞膜流动性变化。将小鼠背部造成１１％～１２％体表面积Ⅲ度烧伤，于伤后第６天取脾淋巴细胞，测其膜流动性。结果表明：烧伤后淋巴细胞膜流动性明显下降（Ｐ＜０．００１），同时淋巴细胞增殖反应及ＣｏｎＡ刺激的ＩＬ－２生成亦下降；淋巴细胞功能受抑制的程度与淋巴细胞膜流动性呈负相关；脾淋巴细胞膜流动性与脾组织过氧化脂质水平呈负相关。结果提示烧伤后淋巴细胞膜流动性下降可能是烧伤后淋巴细胞功能抑制的原因之一，膜流动性降低与脂质过氧化亢进有关     

抗食管癌单克隆抗体及食管癌细胞膜抗原的初步研究

以食管癌“109”细胞株免疫 BALB/C 小鼠,取其脾细胞与小鼠骨髓瘤细胞株SP2/0 融合,经三次克隆化,获得稳定分泌抗食管癌“109”细胞株单克隆抗体的杂交瘤细胞系2GF3。其分泌的单克隆2GF3抗体与外周血淋巴组胞、ABO 型红细胞、癌胚抗原以及3/5胃癌细胞株无反应,与2/5胃癌细胞株有弱(+)反应。与2GF3抗体对应的抗原为2GF3抗原,荧光标记检测表明后者主要表达在细胞膜上。用去污剂提取膜抗原进行电泳后转移至硝酸纤维素膜上进行酶标分析,2GF3抗原是分子量53KD 的糖蛋白,在还原及非还原条件下均显示相同的区带。它与抗癌胚抗原抗体及抗甲胎蛋白抗体均无反应,表明它不同于癌胚抗原和甲胎蛋白。     

带酞基聚芳醚酮超滤膜的研究──Ⅰ带酞基聚芳醚酮超滤膜的制备与性能

以带酞基聚芳醚酮（ＰＥＫＭ）为膜材料，用相转换法制备了ＰＥＫ—Ｃ不对称超滤膜，研究了铸膜液的主要组分对膜的孔结构与超滤性能的影响。     

微量沙眼衣原体组织培养法的建立及临床应用

建立了微量沙眼衣原体组织培养法，并应用于不同人群的生殖道标本的沙眼衣原体培养。结果生殖道沙眼衣原体培养的阳性率：男性尿道炎患者１４７％（９／６１）；正常孕妇３３％（７／２１０）；急性和亚急性盆腔炎女性患者６７％（７／１０４）；性病门诊患者１８２％（４９／２６９）。由于９６孔培养板及倒置荧光显微镜的应用，微量培养法试剂用量少、过程简化；自制的抗沙眼衣原体单克隆抗体大大降低了培养后的染色成本，适用于大宗标本。     

用荧光探剂DPH研究家蝇线粒体膜脂流动性

本文探讨了用ＤＰＨ作为荧光探剂，研究家蝇飞翔肌线粒体膜脂流动性的方法。实验表明：ＤＰＨ确是用来研究家蝇生物膜流动性的有效探剂。同时，初步观察了不同杀虫剂对ＤＰＨ与线粒体膜结合后荧光强度的影响。其中氯菊酯和倍硫磷、马拉硫磷、敌百虫、氧化乐果，可使ＤＰＨ探剂与线粒体膜结合后的荧光相对强度增加；而氰戊菊酯、氯氰菊酯，溴氰菊酯及辛硫磷、乙酰甲胺磷，则可使荧光强度减弱。     

单核细胞流变特性异常在动脉粥样硬化进程中的作用

目的：了解单核细胞流变特性异常在动脉粥样硬化进程的作用。方法：大耳白兔６０只随机分为实验组（ＡＧ）４０只及对照组（ＣＧ）２０只，ＡＧ给予胆固醇ｌｇ．ｄ＾－１只＾－１在喟养的第２，４，８和１２周分别抽血检测单核细胞变形性，膜脂流动性及单核细胞（Ｃａ＾２＋０ｉ。结果：在兔ＡＳ阶段性进程中，单核细胞变形性，膜脂流动性下降趋势，细胞内（Ｃａ＾２＋）ｉ呈上升趋势，膜流动性与细胞内（Ｃａ＾２＋）ｉ呈负相关，变     

Effects of lead on red blood cell membrane proteins

Summary The effects of lead on red blood cell (RBC) membrane proteins were studied in two groups of workers with different lead exposure levels: Group 1 (6 subjects employed in a battery plant) with a mean blood lead of 40.1 (SD = 3.7) g/100 ml; Group II(5 workers employed in different industries) with a mean blood lead of 60.6 (SD = 8.0) g/100 ml, compared with a control group with mean blood lead of 15.6 (SD = 9.3) g/100 ml. The analysis of RBC membrane polypeptides was carried out by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by using a densitometer for percentage measurement of the bands corresponding to protein fractions. The results show a very significant decrease in Band 3 (anion channel) and 4.1 in more exposed workers (Group II) only. The effects of lead on RBC membrane proteins seem to be evident at blood-lead levels higher (> 50 g/100 ml) than those previously reported in literature. These results confirm the effects of lead on membrane proteins, even if the exact mechanism, particularly the influence of proteolysis and the meaning of the interference, still needs to be investigated thoroughly.     

不同来源潜伏膜蛋白1基因转染对鼻咽癌CNE1细胞生长的影响

目的:探讨不同来源的EB病毒潜伏膜蛋白1(LMP1)基因转染对人高分化鼻咽癌(NPC)细胞系CNE1生长的影响。方法:采用脂质体介导法分别将含有来源于B95-8淋巴细胞标准株的LMP1基因(B95-8-LMP1)和来源于NPC组织的LMP1基因(CAO-LMP1)的质粒转染CNE1;RT-PCR和蛋白印迹法分别鉴定LMP1mRNA和蛋白表达;结晶紫法、流式细胞术和平板克隆形成法测定不同来源的LMP1对CNE1生长特性的影响。结果:成功建立稳定表达不同来源LMP1的CNE1细胞系。两种不同来源的LMP1均可促进CNE1细胞的生长(P<0.01);CAO-LMP1比B95-8-LMP1具有更强促进CNE1生长的作用(P<0.01)。结论:不同来源LMP1对CNE1的体外生长均有促进作用,CAO-LMP1比B95-8-LMP1具有更强的促增殖能力。     

Vasopressin induced rhythmic activity in rat basilar artery

The effects of vasopressin on membrane potential and tension were studied in isolated segments of basilar arteries from the University of Iowa colonies of normotensive inbred Kyoto-Wistar rats (WKY) and stroke-prone spontaneously hypertensive rats (SP-SHR). In the presence of vasopressin (0.01–0.3 IU/ml), basilar arteries from WKY, but not from SP-SHR, developed rhythmic contractions. These contractions were recorded in 13 of 14 WKY basilar arteries, were unaffected by pretreatment with 6-hydroxydopamine, and were characterized by 20–100 dyne oscillations in tension, occurring 1–3 cycles/min, and superimposed on the vasopressin-induced contraction (averaging 60 dynes at 0.01 IU/ml or 160 dynes at 0.3 IU/ml). However, resting membrane potentials were not different in SP-SHR vs. WKY at 37°C, and both strains showed about the same (11 mV) depolarization by 0.1 IU/ml of vasopressin. The rhythmic contractions were enhanced by K⁺-free solution, and abolished in the presence of high K⁺ solution (30 mM), suggesting that active Na⁺−K⁺ transport may be involved in modulating the rhythmic activity. These findings are consistent with the hypothesis that the vasopressin-induced rhythmic contractions in WKY basilar arteries are at least partly dependent on a reduced activity of electrogenic Na⁺−K⁺ active transport in WKY as compared to SP-SHR. This research was supported by Grant Nos. HL14388 and HL16328 from the National Institutes of Health. Dr. Rusch is the recipient of Postdoctoral Fellowship HL06907.