响应面法优化替考拉宁发酵培养基及其扩大培养

目的 通过响应面法,优化替考拉宁发酵培养基,并对发酵工艺参数进行优化,来提高其发酵产量。方法 以游动放线菌TC19-3p-103为试验菌株,采用单因素试验确定发酵培养基考察因素的参考范围;利用最陡爬坡试验确定响应面试验的中心区域;利用Box-Behnken响应面法,确定了发酵培养基中的有机氮源最佳浓度组合;并对起始搅拌转速与通气量这两个发酵工艺参数进行单因素考察;在发酵过程中,采用流加技术控制碳源浓度。结果 经优化的发酵培养基,其摇瓶产量提高了31.6%;50L罐发酵工艺参数优化后,发酵水平达到8558 mg/L。结论 优化后的发酵工艺,显著提高了替考拉宁的产量,为其工业化生产奠定了基础。     

全过程质量控制在Ⅰ期临床试验管理中的应用

目的:探讨全过程质量控制在Ⅰ期临床试验管理中的应用效果.方选:选择实施全过程质量控制前(2018年1月-2019年1月)和实施后(2019年6月-2020年6月)的96例受试者和40名研究护士作为研究对象,分别作为对照组和研究组,实施传统的回顾式方法及全过程质量控制质量管理流程,采用量表分析法比较两组受试者依从性、脱落...     

钛板坚强内固定治疗下颌骨骨折136例报告

目的：总结钛板坚强内固定术治疗下颌骨骨折的临床疗效。方法：收集1998～2004年136例下颌骨骨折病例,行钛板坚强内固定术治疗,分别在手术后1个月、3个月、6个月复查,拍摄X线片,观察骨折线对位愈合情况、咬合关系及开口度等功能恢复情况。结果：136例中134例一期愈合,2例延期愈合。X线片示骨折线对位良好,咬合关系及开口度均恢复良好。结论：钛板坚强内固定术治疗下颌骨骨折效果好,方法可靠,操作简单。     

生物等效性试验健康受试者筛选流程的优化

目的 探讨如何提高生物等效性试验健康受试者筛选期的工作效率.方法 试验甲给予受试者根据各个检查项目等待人数自由分流的筛选方法;试验乙给予对部分检查项目进行先后顺序限定,并要求研究者在发现受试者某项检查结果异常后,应及时终止其继续参加其他检查的筛选方法.比较2个试验中受试者在某项检查不合格时合并有其他项目检查的情况.结果...     

Combination of Clavicular Hook Plate with Coracoacromial Ligament Transposition in Treatment of Acromioclavicular Joint Dislocation

ObjectiveTo evaluate the clinical efficacy of Arbeitsgemeinschaft für Osteosynthesefragen (AO) clavicular hook plate (CHP) combined with coracoacromial ligament transposition for Rockwood III‐V dislocation of AC joint, providing an alternative choice for AC joint dislocation treatment.MethodsTwenty‐five patients diagnosed with Rockwood III‐V dislocation of acromioclavicular (AC) joint, including 18 males and seven females, aged 43.5 ± 2.4 years old on average, who had undergone open reduction and AO CHP in combination with coracoacromial (CC) ligament transposition between January 2010 and December 2015, were retrospectively analyzed. Among them, 17 cases were diagnosed as type III, five cases were type IV, and three cases were type V. The surgery mainly included three main steps: bone flap incision, drilling in the clavicle, and hook plate fixation and AC joint reposition. The treatment efficacy was evaluated through clinical examinations and imaging studies for the shoulder joint, including gross observation and measuring coracoid clavicle distance (CC‐Dist) using orthophoria X‐ray before and 1 year after the surgery, and University of California (UCLA) shoulder rating scale.ResultsAll the patients were followed up three to four times in 18 months (12–24 months) on average, and the UCLA rating results showed that there were 17 excellent cases (68%), five good cases (20%), and three fair cases (12%). The CC‐Dist values after the surgery reduced to 9.7 ± 0.7 mm, which was significantly (P < 0.05) lower compared to that before the surgery (15.8 ± 1.6 mm). Most (88%) of the cases showed almost normal joint function and good anatomical arrangement of the acromioclavicular joint, without any secondary dislocation, and for them, 12 ± 2 weeks on average were needed to regain the normal function of shoulder joint movement.ConclusionDue to the stable fixation, fewer complications, and satisfactory therapeutic effect with great clinical value, the combination of AO CHP and CC ligament transposition is expected to be used for treating Rockwood III‐V dislocation of AC joint.     

解剖型钢板治疗胫骨远端骨折

胫腓骨骨折发生率很高,约占长骨骨折的10％。胫腓骨远端骨折在胫腓骨骨折中最为常见,由于涉及干骺端的骨折,髓内钉治疗有其局限性。2002年3月～2003年7月,笔者采用解剖型钢板治疗胫骨远端骨折12例,取得了良好疗效。     

急诊解剖型钢板治疗胫骨远端骨折

目的探讨急诊解剖型钢板治疗胫骨远端骨折的临床效果。方法82例胫骨远端骨折患者急诊采用解剖型钢板手术治疗,按AO/ASIF分型:A1型25例,A2型27例,A3型21例,B1型9例。结果82例获得6~20个月的随访,骨折全部愈合,无钢板螺钉断裂、退出等,表浅感染3例。踝关节功能评价参照Mazur标准,优良率93·9%。结论解剖型钢板手术简单,固定牢固,是急诊治疗胫骨下段骨折的一种较好内固定方法。     

锁骨钩钢板内固定治疗NeerⅡ型锁骨远端骨折

2001年3月～2004年4月,我院应用锁骨钩钢板治疗NeerⅡ型锁骨远端骨折,疗效满意。1材料与方法1.1病例资料本组25例,男14例,女11例,年龄13～65岁。左侧9例,右侧16例;横形13例,斜形12例。均为NeerⅡ型新鲜骨折,其中ⅡB型骨折7例。受伤至入院时间30min～6d。X线片显示均为锁骨外1/3骨折。1.2治疗方法颈丛麻醉,采用改良Herry手术切口。行骨折复位,用锁骨钩钢板的钩端钩住肩峰后下方,外展及上举肩关节使骨折端紧密嵌合,钛板塑形预弯后紧贴锁骨上方的骨皮质,用松质骨和皮质骨螺钉分别固定远近骨折端,重叠缝合三角肌及斜方肌纤维脚。对ⅡB型喙锁…     

Optimal Design of Double-Walled Corrugated Board Packaging

Designing corrugated board packaging is a real challenge, especially when the packaging material comes from multiple recycling. Recycling itself is a pro-ecological and absolutely necessary process, but the mechanical properties of materials that are processed many times deteriorate with the number of cycles. Manufacturers are trying to use unprecedented design methods to preserve the load-bearing capacity of packaging, even when the material itself is of deteriorating quality. An additional obstacle in the process of designing the structure of paper packaging is the progressive systematic reduction of the grammage (the so-called lightweight process) of corrugated cardboard. Therefore, this research presents a critical look at the process of optimal selection of corrugated cardboard for packaging structures, depending on the paper used. The study utilizes analytical, simplified formulas to estimate the strength of cardboard itself as well as the strength of packaging, which are then analyzed to determine their sensitivity to changes in cardboard components, such as the types of paper of individual layers. In the performed sensitivity analysis, numerical homogenization was used, and the influence of initial imperfections on the packaging mechanics was determined. The paper presents a simple algorithm for the optimal selection of the composition of corrugated cardboard depending on the material used and the geometry of the packaging, which allows for a more conscious production of corrugated cardboard from materials derived, e.g., from multiple recycling.     

股骨近端解剖型锁定钢板治疗老年股骨转子间骨折

2008年6月～2010年5月,我科采用大转子直切口股骨近端解剖型锁定钢板治疗23例老年股骨转子间骨折患者,取得满意疗效。1材料与方法1.1病例资料本组23例,男9例,女14例,年龄65～85岁。伤后至手术时间3～10 d。左侧10例,右侧13例,按