中下面部线雕联合局部注射填充对皮肤状态及不良反应的影响

目的 探究在面部年轻化治疗中采用中下面部线雕联合局部注射填充的效果及对皮肤状态的影响。 方法 选取2021年1月-2024年1月于观山湖科珑小可美容诊所美容皮肤科行面部年轻化治疗的60例患者, 随机分为对照组和观察组,每组30例。对照组采用局部注射填充治疗,观察组采用中下面部线雕联合局 部注射填充治疗,比较两组皮肤状态、心理状态、生活质量及不良反应发生情况。结果 观察组术后皮 肤水分、弹性评分高于对照组,油脂、暗斑评分低于对照组（P ＜0.05）;观察组术后SAS、SDS评分均 低于对照组（P ＜0.05）;观察组术后生活质量各维度评分均高于对照组（P ＜0.05）;观察组不良反应发 生率为6.67%,低于对照组的30.00%（P＜0.05）。结论 中下面部线雕联合局部注射填充在面部年轻化中的 应用效果确切,抗衰效果及美学效果良好,可有效改善皮肤质地,减少皱纹,且治疗后不良反应较少,安 全性较高,有利于改善患者的心理状态及生活质量,值得临床应用。     

NT-3、NT-4和BDNF在面神经逆行转运的动力学比较

目的　研究神经营养素 3(NT- 3)、神经营养素 4(NT- 4)和脑源性神经营养因子 (BDNF)在面神经中的逆行转运动力学特点。方法　将新西兰兔一侧面神经干横切断后置入硅胶再生室 ,再将 1 2 5I标记的 NT- 3或NT- 4或 BDNF或人血清白蛋白 (HSA) (10μl/只 ,约 3.7MBq)注入再生室 ,在注药后于不同时刻取兔面神经干和脑干面神经运动核 ,测定其摄取率。利用 3P87动力学处理程序分析计算各标记物在面神经的动力学参数。结果　面神经逆行转运神经营养素的转运量 :NT- 3>BDNF>NT- 4(P <0 .0 5 ) ,转运速率 :NT- 4>NT- 3>BDNF (P <0 .0 5 )。结论　研究结果提供了神经营养素在面神经逆行转运的动力学特点。     

振动负荷前后家兔神经传导速度变化的实验研究

本文以５２只家兔为实验对象，按不同的接振剂量分为五个组（其中包括一对照组），采用后肢接振法来模拟人体局部振动作业状态，振动实验６０天，使用丹麦产Ｎｅｕｒｏｍａｔｉｃ２０００Ｃ型神经－肌动描记仪，在实验前、实验后１５天、３０天和６０天分别测定家兔在侧坐骨神经ＳＣＶ和ＭＣＶ。实验结果发现，接振组ＳＣＶ和ＭＣＶ均较对照组明显减慢，尤其是ＳＣＶ改变更为明显，与人体局部振动作业所致外周神经损伤检查结果有一致     

神经再生素对皮层细胞基因表达影响的基因芯片研究

目的：采用基因芯片技术,观察神经再生素对体外培养的胚鼠大脑皮质细胞基因表达的影响。方法：取ICR胚鼠（15d)大脑皮层细胞,无血清培养。实验组加入神经再生素（2μg/ml）,对照组加入等量基础培养液。培养6h后,抽提实验组和对照组皮层细胞总RNA,经反转录分别用Cy5、Cy3荧光标记成cDNA探针。将荧光标记的cDNA与MGEC－40s基因表达谱芯片杂交,芯片扫描、数据处理。结果：实验组与对照组大脑皮质细胞出现64条差异性表达的基因。呈现上调的基因有：钙调素结合蛋白、锌指蛋白激酶、核糖体蛋白等基因;下调基因有：抑制细胞分裂因子等基因。结论：神经再生素可作用于体外培养的胚鼠大脑皮层细胞,从基因调控水平促进脑细胞生长。     

眉区不同部位切口的选择与应用

目的 为眉周美容手术提供一个理想的切口选择.方法 根据患者的脸型、眉区情况、患者的治疗目的,要解决那些问题进行评估制定方案,选择不同切口:A、全眉切口;B、部分眉切口;C、眉上切口;D、眉下切口;E、眉内切口.结果 本组158例,均为Ⅰ期愈合,3个月至半年均可稳定,晚期眉切口痕迹难以察觉,效果满意.结论 通过眉切口可以进行多种面部美容手术,也能进行多种美容项目的联合手术,我们认为,它已成为面部上1/3美容手术的主要切口.     

The Sensational Hand: Clinical Assessment after Nerve Repair

Following the transection and repair of major nerve trunks in the forearm, the functional outcome is influenced by mechanisms in the peripheral, as well as in the central nervous system. In the present thesis the interest is focused on assessment of the outcome after nerve repair, central nervous factors influencing the outcome, and sense substitution to compensate for sensory loss. A new model instrument for routine documentation of the outcome after repair of a peripheral nerve is identified. The model includes assessments reflecting sensory, motor and pain/discomfort domains. Investigations of frequently used assessment instruments led to the construction and evaluation of a new test instrument for assessment of discriminative sensibility (tactile gnosis) to fit in the model. The summarised outcome, calculated from the model instrument and with a numerical scoring system, conforms well with the patient's opinion on the influence on activities of daily living resulting from the nerve injury, and demonstrates good reliability and validity. A reference interval for the outcome is presented, with the estimated 95% predicted values for the outcome up to five years after the nerve repair. Brain plasticity is a factor sensibility - tactile gnosis - in the adaptive process after a nerve injury, when the mind has to interpret new signal patterns, when objects are touched. For better understanding of the sensory outcome after nerve repair, central nervous factors were examined. Specific cognitive capacities, such as verbal learning and visuo-spatial logic capacity could be identified as being of importance for recovery of tactile gnosis. For patients with temporary or permanent sensory loss, a new principle for artificial sensibility based on sense substitution is presented. The hearing sense substitutes the sense of touch. The resemblance in perceptual experience between sound and touch is bridged by the stereophonic friction sound generated by touching objects, which is then amplified and transmitted to earphones. The delicate capacity of the sense of hearing to discriminate between the complex pattern of frequencies makes it reasonable to assume that hearing is able to take over functions normally devoted to touch. This is demonstrated in the thesis.     

Auditory Neurotoxicity and Hepatotoxicity After MSMA (Monosodium Methanarsenate) High Dose Oral Intake

Context.?Human butyrylcholinesterase (huBuChE) has potential utility as a post-exposure therapy following percutaneous nerve agent poisoning as there is a slower absorption of agent by this route and hence a later onset of poisoning. Methods.?We used surgically implanted radiotelemetry devices to monitor heart rate, EEG, body temperature and locomotor activity in guinea pigs challenged with VX via the percutaneous route. Results.?Treatment with huBuChE (24.2 mg/kg, i.m.) at 30 or 120 min following percutaneous VX (~2.5 × LD₅₀) protected 9 out of 10 animals from lethality. When i.m. huBuChE administration was delayed until the onset of observable signs of systemic cholinergic poisoning, only one out of six animals survived to 7 days. Survival increased to 50% when the same dose of huBuChE was given intravenously at the onset of signs of poisoning. This dose represents approximately 1/10th the stoichiometric equivalent of the dose of VX administered (0.74 mg/kg). Intramuscular administration of huBuChE (24.2 mg/kg) alone did not produce any changes in heart rate, brain electrical activity, temperature or locomotion compared to saline control. Survival following VX and huBuChE treatment was associated with minimal incapacitation and observable signs of poisoning, and the mitigation or prevention of detrimental physiological changes (e.g. seizure, bradycardia and hypothermia) observed in VX + saline-treated animals. At 7 days, cholinesterase activity in the erythrocytes and most brain areas of guinea pigs that received huBuChE at either 18 h prior to or 30 min following VX was not significantly different from that of naïve, weight-matched control animals. Conclusion.?Percutaneous VX poisoning was successfully treated using post-exposure therapy with huBuChE bioscavenger. The opportunity for post-exposure treatment may have particular relevance in civilian settings, and this is a promising indication for the use of huBuChE.     

梅花针叩刺治疗脾肾两虚夹瘀型糖尿病周围神经病变的临床观察

目的：探讨梅花针叩刺治疗脾肾两虚夹瘀型糖尿病周围神经病变(DPN)的临床效果。方法：选取2015年2月至2018年1月广州市番禺区中医院收治的脾肾两虚夹瘀型DPN患者110例作为研究对象，随机分为对照组(n＝50)与观察组(n＝60)。对照组给予硫辛酸+甲钴胺治疗，观察组在对照组治疗的基础上给予梅花针叩刺治疗，2组均治疗观察28 d，记录并观察2组的疗效和预后。结果：观察组的总有效率为98.3%，显著高于对照组的88.0%(P<0.05)。治疗后2组的神经电图潜伏期低于治疗前，且波幅高于治疗前，2组比较差异有统计学意义(P<0.05)。2组治疗后正中神经和腓总神经的MNCV、SNCV高于治疗前，且观察组高于对照组，差异均有统计学意义(P<0.05)。治疗后6个月随访，观察组的复发率为1.7%，显著低于对照组的14.0%，差异有统计学意义(P<0.05)。结论：梅花针叩刺治疗脾肾两虚夹瘀型DPN能改善神经功能指标，提高治疗效果，降低远期复发率。     

外伤性面瘫影像表现与手术对照

目的 分析面神经(管)损伤的CT和MRI表现. 资料与方法 对外伤性面瘫患者的20侧耳进行CT检查,其中6侧耳同时行MRI检查,对照手术结果 回顾性分析影像学表现. 结果 CT显示面神经管鼓室段损伤8耳,迷路段损伤1耳,乳突段损伤1耳,前膝和鼓室段同时损伤3耳,前膝和迷路段同时损伤2耳,迷路段和鼓室段同时损伤1耳,无异常表现4耳.行MRI的6侧耳表现为面神经鼓室段损伤2耳,乳突段损伤1耳,鼓室段和乳突段同时损伤1耳,迷路段、前膝和鼓室段同时损伤2耳. 结论 CT能较准确地显示面神经管的骨折,但不能完全明确面神经损伤的范围,而MRI明确地显示面神经的损伤范围,但不能直接显示骨折本身,两者联合使用可明确诊断面神经损伤范围及骨折.     

眼轮匝肌在睁、闭眼运动中的功能作用分析

目的：分析眼轮匝肌在睁、闭眼运动中的功能作用。方法：通过植入式微电极提取狗眼轮匝肌的肌电信号,从中分析该肌肉在睁、闭眼运动中的功能作用状态。结果：眼轮匝肌在自然睁眼状态中与自然闭眼状态中的肌电表现一致;在眨眼动作发生过程中肌电活动有增强表现;在由睁眼状态向闭眼状态转换过程中,肌电活动在闭眼动作发生时有增强表现;在由闭眼状态向睁眼状态转换过程中,没有观察到肌电信号有变化。结论：眨眼动作的发生需要眼轮匝肌的收缩作用来完成;在由睁眼状态向闭眼状态转换,需要眼轮匝肌的收缩来使眼睑进入闭合状态;闭眼状态不需要眼轮匝肌的持续收缩来维持。