内皮素－1对大鼠黄体细胞孕酮生成的影响

我们采用黄体细胞孵育的方法，观察了内皮素－１对离体黄体细胞孕酮生成的影响。发现内皮素－１能抑制黄体细胞孕酮的基础分泌及人绒毛膜促性腺激素（ＨＣＧ）刺激下的孕酮分泌。内皮素－１加入孵育的黄体细胞悬液１５分钟后，即发生明显地抑制孕酮分泌作用（Ｐ＜０．０１）。人内皮素－１的抗血清能反转内皮素－１的上述作用。实验结果提示，内皮素－１可能为一种卵巢内调节肽，它通过抑制黄体细胞的孕酮生成，参与黄体退化过程的调节     

血管内皮素对人类血小板功能影响的研究

实验表明,血管内皮素不引起血小板胞浆游离钙离子的变化和血小板聚集;血管内皮素与血小板活化剂(胶原、凝血酶、血小板激活因子)联合实验表明,血管内皮素对血小板活化剂引起的反应亦无明显影响。     

益气活血法治疗频发的短暂性脑缺血发作临床研究

目的　旨在观察益气活血法对频发的短暂性脑缺血患者的临床疗效 ,并初步探讨其作用机制。方法　本文选择TIA病人 5 8例 ,随机分为益气活血组和单纯活血化瘀组 ,治疗前后分别测定患者血浆内皮素 (ET)、一氧化氮 (NO)水平及凝血酶原时间 (PT)、部分凝血酶原时间 (APTT)和纤维蛋白原 (FIB)含量 ,并与正常健康人群作对照研究。结果　急性脑缺血发作时患者血浆ET、NO水平明显升高 (P <0 0 1) ,治疗后ET可下降 ,NO有升高趋势 ,ET NO下降 ,而益气活血组下降程度明显高于活血化瘀组 (P <0 0 1)。而PT、APTT和FIB变化不大。结论　提示改善血管内皮功能 ,调节ET NO之间的动态平衡 ,从而保护缺血性脑组织的损伤 ,是益气活血法治疗TIA的主要作用机制之一。     

Validation of endothelin (ET) immunoreactivity in human bile by HPLC. Comparison of biliary ET concentration in liver transplant recipients with values obtained during cholecystectomy

Abstract  High endothelin (ET) concentrations were recently detected in human bile after ortho-topic liver transplantation (OLT). In the present study we compared biliary ET/big-ET levels measured by radioimmunoassay (RIA) in liver graft recipients ( n = 37) with levels measured in non-transplant patients during cholecystectomy ( n = 38) to clarify the influence of transplantation on the levels of biliary ET pep-tides. HPLC elution profiles of biliary ET were analyzed for characterization of ET peptide composition and validation of RIA analysis in bile extracts. Mean ET/big-ET levels in the common bile duct after OLT were significantly elevated (ET, 20.9 ± 15; big-ET, 39.2 ± 19 fmol/ml) compared to levels in non-transplant patients (ET, 5.7 ± 4.9; big-ET, 12 ± 8 fmol/ml). Highest ET/big-ET levels were measured in the gall bladder during cholecystectomy (ET, 61.7 ± 41; big-ET, 75 ± 28 fmol/ml). ETand big-ET levels were correlated by linear regression. HPLC analysis reveals the presence of high levels of ET/big-ET in human bile. Biliary ET mostly represents ET-I. High biliary ET levels after OLT appear to be derived from active endothelial secretion and probably reflect hepatic endothelial stress after preservation/ reperfusion. High biliary ET levels could be involved in the mediation of functional cholestatic syndromes after OLT.     

EFFECT OF ELECTROACUPUNCTURE ON CEREBRAL BLOOD FLOW, VIP AND ET IN RATS WITH VASCULAR DEMENTIA

Cerebralischemiainducedbydisturbanceofcerebralcirculationisoneofthemajorfactorsforvasculardementia (VD) .Mostresearcherscon sideredthattheonsetofVDisusuallycloselyre latedtothelesionofcerebralcortex ,especially ,totheischemiaintheleftcortex ,thalamusandhippocampus.Previousstudiesshowedthatacupuncturecouldrapidlyincreasecerebralbloodflowintheischemicregion ,reducetheinfarctvolumeofthebrainandsoastopreventthener vousfunctionfromimpairment[1 ] .However,therewerefewerstudiesontherelationshipbe t…     

Neural and vascular regulatory factors of the skin

Background Neural and vascular factors regulate many aspects of dermal function, including blood flow, inflammation, tissue repair and eccrine secretion. Abnormalities of neurovascular regulation may contribute to dermatological manifestations of a wide range of diseases including psoriasis, nodular prurigo and leprosy, diabetes mellitus and Raynaud's disease. Aims In this review we discuss evidence for roles played by neural and vascular regulatory factors in skin, with particular reference to the regulatory peptides substance P, calcitonin gene-related peptide. vasoactive intestinal polypeptide, neuropeptide Y and endothelin and to the important endothelial cell product nitric oxide. Conclusions An understanding of the anatomical basis of neurovascular regulation and the changes which occur during disease may lead to novel diagnostic and prognostic tests for dermatological conditions, while an understanding of specific receptors mediating the actions of regulatory peptides will lead to the development of specific antagonists as a novel approach in clinical dermatology.     

电离辐射对血管内皮细胞影响的实验研究

目的　观察电离辐射 (X线 )对血管内皮细胞培养上清液中内皮素 (ET)和一氧化氮 (NO)含量变化及细胞凋亡的影响。方法　采用新生儿脐带静脉血管内皮细胞 (HUVEC)体外原代培养方法 ,用放免法和化学法检测培养上清液中ET和NO含量 ,用透射电镜、流式细胞仪观察细胞形态特征和检测凋亡细胞。结果　血管内皮细胞在电离辐射 2 4h后 ,各辐射剂量组培养上清液中NO含量无变化 ;在 2Gy剂量组ET含量变化不明显 ,而 5、10、2 0Gy各剂量组ET含量明显下降 (P <0 .0 5 )。电离辐射 2 4、4 8h后HUVEC形态无明显改变 ,未检测出明显凋亡细胞。结论　原代培养生长的HUVEC在 2～ 2 0Gy照射时有一定的放射抗性 ,其确切机理有待进一步研究     

烧伤后肾组织内皮素-1及其受体基因的表达

目的探讨烧伤后肾组织内皮素-1(ET-1)升高的效应部位以及效应的 ETR 亚型。方法采用斑点杂交和原位杂交技术对大鼠30％体表面积Ⅲ度烧伤模型的 ET-1及其受体亚型(ETA、ETB)的基因表达进行了研究。结果烧伤后1小时,ET-1、ETA、ETB、mRNA 均明显升高;烧伤后24小时仍维持较高水平。ET-1和 ETA mRNA 杂交信号于烧伤后3小时达高峰。ET-1mRNA 主要分布肾皮质小血管内皮细胞、髓质肾小管和集合管,ETA 受体 mRNA 则分布于上述小血管的平滑肌细胞。ETB 受体mRNA 于烧伤后6小时达高峰,主要分布髓质肾小管、集合管。结论烧伤后肾脏内皮素受体亚型上调在皮质以 ETA 为主,在髓质以 ETB 为主,它们分别与增强表达的 ET-1结合可能导致肾皮质缺血和水钠代谢异常,是烧伤后肾功能障碍的重要因素。     

氧化修饰低密度脂蛋白及内皮素-1水平与消化性溃疡

为探讨低密度脂蛋白的氧化修饰程度及内皮素分泌情况在胃及十二指肠溃疡发病中的作用及其机理，用单克隆抗体酶联免疫吸附法和放射免疫分析均相竞争法分别测定了血浆中氧化修饰低密度脂蛋白和内皮素－１的含量。结果：胃及十二指肠溃疡患者血浆中的氧化修饰低密度脂蛋白水平分别为（０．５２±０．０９）ｍｇ／Ｌ和（０．６７±０．０９）ｍｇ／Ｌ，均较正常人（０．３２±０．０７）ｍｇ／Ｌ呈显著升高（Ｐ＜０．０１），内皮素－１水平在胃及十二指肠溃疡时分别为（１０．２±２．３）ｎｇ／Ｌ和（１３．１±２．８）ｎｇ／Ｌ，也较正常人（６．０±１．３）ｎｇ／Ｌ明显地增高（Ｐ＜０．０１）；两项指标在胃溃疡患者均明显地低于十二指肠溃疡患者（Ｐ＜０．０５）；且两指标的总体水平均与溃疡直径呈明显正相关（Ｐ＜０．０５）。提示氧化修饰低密度脂蛋白和内皮素－１可能参与了溃疡的形成过程，并有可能是造成溃疡的重要因素。     

甲状腺功能亢进症治疗前后血浆内皮素水平的变化

观察甲状腺功能亢进症治疗前后血浆内皮素水平的变化。方法：采用放射免疫分析法测定４５例Ｇｒａｖｅｓ病患者治疗前后及２５例健康人的血浆ＥＴ－１水平。