FK506肾毒性的预防

目的 筛选拮抗ＦＫ５０６所致肾毒性的药物。方法 将卡托普利,内皮素受体拮抗剂及钙通道阻滞剂３种药物分别与ＦＫ５０６一起进行大鼠离体肾灌流研究,观察３种药物对ＦＫ５０６致肾灌流阻力升高及肾小球滤过率下降的影响。结果 卡托普利对肾灌流主肾小球滤过率的变化无影响;     

Endothelin receptor antagonist BQ-123 ameliorates myocardial ischemic-reperfusion injury in rats: A hemodynamic,biochemical, histopathological and electron microscopic evidence

We investigated the effect of BQ-123, a selective endothelin-A (ET_A) receptor antagonist in ischemia-reperfusion (IR) induced myocardial infarction (MI) with and without endothelin-1 (ET-1) challenge. MI was produced in rats by occlusion of left anterior descending coronary artery for 40 min and reperfusion for 120 min. ET-1 was administered immediately prior to coronary occlusion whereas vehicle or BQ-123 was administered 20 min after the occlusion. IR control group exhibited marked hemodynamic changes along with significant impairment of left ventricular functions. In addition, oxidative stress was increased, as evidenced by marked reduction in the activities of antioxidants and cardiac injury markers in myocardium. Furthermore, light microscopic and ultrastructural changes revealed myocardial necrosis, edema and inflammation. Prior administration of ET-1 acts synergistically with IR injury and further aggravates the impairment of ventricular functions, increased percent infarct area and decreased antioxidant levels. However, treatment with BQ-123 (1 mg/kg, IV) with or without ET-1 caused significant improvement in cardiac functions, percent infarct area, decreased malonaldehyde level, restored myocardial enzymes activities and maintained the redox status of the myocardium as compared to IR control group. Further, histopathological and ultrastructural studies reconfirmed the protective action of BQ-123. The results of present study suggest that ET-1 acting via ET_A receptor may exaggerate myocardial damage produced by IR injury and selective blockade of ET_A receptor by BQ-123 might offer potential cardioprotective action.     

复方丹参注射液治疗急性胰腺炎的临床研究

目的观察复方丹参注射液在治疗急性胰腺炎中的临床疗效,了解复方丹参注射液对急性胰腺炎（AP）患者血浆内皮素（ET）浓度的影响,对改善微循环的作用。方法将100例AP患者随机分为对照组（40例）和治疗组（60例）,对照组采用常规治疗,治疗组采用常规治疗＋复方丹参注射液治疗,以放射免疫法（RIA）测定血浆ET浓度。结果两组病例除了腹痛消失时间无显著性差异外（P〉0．05）,腹部压痛消失、血尿淀粉酶、影像学恢复和平均治疗时间治疗组均短于对照组（P〈0．05）。治疗7d后,对照组患者血浆ET浓度与治疗前比较无显著性差异;治疗组患者治疗后血浆ET浓度[（61．89±7．14）ng／L]显著低于治疗前[（104．41±7．88）,P〈0．05]。结论在常规治疗的基础上应用复方丹参注射液治疗急性胰腺炎可以使患者微循环状态得到改善,具有良好的临床疗效。     

参七胶囊对冠心病患者血浆内皮素和一氧化氮的影响

目的　观察参七胶囊对冠心病患者血浆内皮素和一氧化氮的影响 ,探讨参七胶囊治疗心肌缺血的机制。方法　 6 0例冠心病患者随机分为 2组 ,对照组 30例应用长效异乐定和肠溶阿司匹林治疗 ,治疗组30例在对照组治疗基础上加参七胶囊治疗 ,连续用药 4周。结果　治疗后治疗组临床症状积分、内皮素下降幅度大于对照组 (P <0 .0 1) ,一氧化氮上升幅度高于对照组 (P <0 .0 1)。结论　参七胶囊具有调节血浆内皮素和一氧化氮浓度的作用 ,这可能是参七胶囊治疗心肌缺血的机制之一     

半夏白术天麻汤联合血府逐瘀汤对高血压患者内皮功能的影响

目的:探讨半夏白术天麻汤联合血府逐瘀汤对高血压患者内皮功能的影响。方法:选取2015年1月至2017年6月河西学院附属张掖人民医院收治的高血压患者80例作为研究对象,按照随机数字表法分为对照组和观察组,每组40例。对照组给予常规西药治疗,观察组在对照组的基础上给予半夏白术天麻汤联合血府逐瘀汤进行治疗,观察和比较治疗后2组患者的血管内皮因子一氧化氮(NO)和内皮素-1(ET-1)水平、疗效、血压以及中医证候积分情况。结果:治疗后,观察组患者治疗有效率为92. 50%,对照组患者的治疗有效率为72. 50%,差异有统计学意义(P 0. 05);治疗后,2组患者舒张压和收缩压均下降,且观察组舒张压和收缩压下降较对照组显著,差异有统计学意义(P 0. 05); 2组患者的血管内皮因子NO水平升高,而ET-1水平下降,且观察组血管内皮因子水平的变化较对照组明显,差异有统计学意义(P 0. 05)。治疗后,2组患者中医证候积分均下降,且观察组中医证候积分下降较对照组显著,差异有统计学意义(P 0. 05)。结论:半夏白术天麻汤联合血府逐瘀汤能显著升高高血压患者的血清NO水平、降低ET-1水平,有效降低患者血压,具有较好的临床效果。     

卡托普利对犬围手术期心肌缺血的预防作用

目的：围手术期心肌缺血主要因应激致冠脉内皮功能障碍，故设想卡托普利可预防其发生。方法：杂种犬４０只随机分为４组：Ｉ组（对照组），Ⅱ组（心梗模型），Ⅲ组（心梗＋胃大部切除术）及Ⅳ组（心梗＋卡托普利＋胃大部切除术）。Ⅲ组及Ⅳ组在心梗后２周行胃大部切除术，测基础值、术前和术后的各指标，用原位杂交观察４组冠脉内皮一氧化氮合酶（ＮＯＳ）ｍＲＮＡ的表达。结果：在Ⅲ组，手术使ＬＶ＋ｄＰ／ｄｔ ｍａｘ、ＣＩ及一氧     

血浆NO和ET—1在内毒素休克进程中的动态变化及其与休克发病的关系

观察内毒休克进程中，家兔血浆ＮＯ的内皮素浓度的动态变化，探讨两者变化规律及可能的致病作用。方法采用离子色谱法及放射免疫法分别检测血浆ＮＯ、ＥＴ含量并记录血流动力学各项指标。     

Endothelin Potentiates Growth Factor-stimulated DNA Synthesis in Swiss 3T3 Cells

A murine cell line, Swiss 3T3, is commonly used for the study of cellular growth. The present study revealed that this cell line possesses high-affinity receptors for endothelin, a vasoactive peptide derived from vascular endothelial cells. In this cell line, endothelin potentiated DNA synthesis stimulated by platelet-derived growth factor, basic fibroblast growth factor or insulin. The endothelin concentration required for potentiating DNA synthesis in this cell line is almost the same as that reported previously in endothelin-induced vasoconstriction. Since endothelin increased intracellular calcium levels, this ion may participate in the signal transduction pathways by which endothelin induces potentiation of DNA synthesis.