EP9-A2对两种发光免疫分析法的一致性分析

目的对EP9-A2法在微粒子化学发光免疫分析法和电化学发光免疫分析法的一致性研究中的应用情况进行分析探讨。方法抽取在2011年3月至2013年3月间我院收集的血清标本63份,对其采取微粒子化学发光免疫分析法和电化学发光免疫分析法对IgE水平进行检测,而后采取EP9-A2法并对检测结果一致性进行分析。结果微粒子化学发光免疫分析法和电化学发光免疫分析法的检测结果存在显著线性关系,结果偏差在允许范围内。结论 EP9-A2法在微粒子化学发光免疫分析法和电化学发光免疫分析法的一致性研究中具有重要价值,值得关注。     

电化学发光法与放射免疫分析法检测血清甲状腺素的比较

目的探讨电化学发光法与放射免疫法检测血清甲状腺激素的效果差异,为临床甲状腺激素检测提供参考。方法选择我院普外科及内分泌科住院甲状腺疾病患者血清标本340份,分别采用电化学发光法及放射免疫法进行检测,比较两种方法检测血清TSH、FT3、FT4线性范围,相关性及精密度。结果电化学发光法检测血清TSH、FT3、FT4线性范围宽于放射免疫法,两种检测方法检测血清TSH、FT3、FT4具有良好的相关性,电化学发光法检测血清TSH、FT3、FT4精密度高于放射免疫法。结论电化学发光法与放射免疫法检测血清甲状腺激素能够取得相同的检测效果,电化学发光法检测方法简单,线性范围较宽,可重复性较好,更适合临床采用。     

标本溶血对电化学发光免疫法结果的影响

目的探讨标本不同程度溶血对电化学发光免疫法(ECLIA)测定结果的影响。方法将血液标本人为干预成不同程度的溶血标本,使用ECLIA检测各组标本中的甲状腺功能、肿瘤标志物、内分泌激素等共28项指标的浓度并做分析。结果标本溶血可使胰岛素(INS)浓度降低,使叶酸(FA)、神经元特异性烯醇化酶(NSE)浓度升高,重度溶血有使铁蛋白测定结果偏高的趋势,但差异无统计学意义,其余指标测定结果不受溶血的影响。结论除某些项目外大部分溶血标本的ECLIA测定结果没有受到影响。测定INS、FA、NSE时应绝对避免使用溶血标本,以确保结果准确。     

Comparison between a new multiplex electrochemiluminescence assay and the WHO reference enzyme-linked immunosorbent assay to measure serum antibodies against pneumococcal serotype-specific polysaccharides

Background

Two electrochemiluminescence (ECL) assays were developed which, together, can simultaneously measure serum antibodies against pneumococcal capsular polysaccharides (PnPS) for 17 serotypes. The assays were validated for the 13 PnPS included in the 13-valent pneumococcal conjugate vaccine (PCV13). As recommended by the World Health Organization (WHO), we compared the ECL assays with the WHO reference enzyme-linked immunosorbent assay (ELISA) and derived a threshold corresponding to the 0.35?µg/mL threshold established for the WHO reference ELISA for the non-inferiority comparison and licensure of new PCVs against invasive pneumococcal disease.

永康地区正常妊娠妇女甲状腺功能指标参考值的研究

目的建立永康市正常孕妇妊娠各期甲状腺功能指标的参考值。方法收集2017年6月-12月在永康市妇幼保健院产检妊娠妇女为研究对象,经过筛选共纳入449例。采用全自动电化学发光免疫分析方法进行甲状腺功能7个项目的测定,并依测定数据统计分析得到各个项目的双侧95%医学参考值范围。结果研究获得本院正常妊娠妇女各孕期甲状腺激素的参考值如下:早孕期,TSH:0.04μIU/ml^4.14μIU/ml,FT4:1.01 ng/dl^1.78 ng/dl,FT3:2.52 pg/ml^4.31 pg/ml;中孕期,TSH:0.02μIU/ml^4.77μIU/ml,FT4:0.85 ng/dl^1.71 ng/dl,FT3:2.34 pg/ml^4.39 pg/ml;晚孕期,TSH:0.24μIU/ml^5.63μIU/ml,FT4:0.74 ng/dl^1.30 ng/dl,FT3:2.11 pg/ml^3.58 pg/ml。年龄对妊娠妇女甲状腺功能影响并不大。根据美国甲状腺协会推荐标准和本研究的参考值诊断本院妊娠妇女亚临床甲状腺功能减退的患病率分别为17.59%和2.23%。结论甲状腺激素在妊娠各期之间的差异有统计学意义,不同地区妊娠妇女甲状腺激素参考值也存在差异,建立适合本地区的特异性甲状腺激素参考值有助于妊娠期甲状腺疾病的诊疗。     

电化学发光免疫测定法检测抗环瓜氨酸肽抗体的方法比较

目的:比较电化学发光免疫测定(ECLIA)法与酶联免疫吸附试验(ELISA)法检测抗环瓜氨酸肽(抗CCP)抗体的价值。方法:采用ECLIA法和ELISA法分别检测192例类风湿关节炎(RA)患者、56例其他风湿性疾病患者及100名健康对照者血清中的抗CCP抗体,观察ECLIA法检测抗CCP抗体的精密度、回收率、诊断灵敏度、诊断特异度,并分析ECLIA法与ELISA法间的相关性。结果:ECLIA法检测抗CCP抗体的批内、批间变异系数分别为0.9%~1.8%、1.7%~2.4%,明显低于ELISA法(5.8%~8.6%,6.7%~9.8%)(P<0.01)。ECLIA法检测抗CCP抗体的回收率为96.7%~98.6%,灵敏度和特异度分别为72.9%、98.7%,与ELISA法检测结果的一致率达96.7%。结论:ECLIA法检测抗CCP抗体的精密度较高,且对RA诊断的灵敏度、特异度与ELISA法具有较好的一致性。     

Ionic liquid-based hollow fiber-supported liquid-phase microextraction enhanced electrically for the determination of neutral red

A method of ionic liquid-based hollow fiber liquid-phase microextraction enhanced electrically was successfully developed and applied to the extraction and determination of neutral red (NR) dye, which was selected as the model analyte. A room temperature ionic liquid, 1-octyl-3-methylimidazolium hexafluorophosphate ([C₈mim][PF₆]), was placed in the pores of a polytetrafluoethylene hollow fiber, which acts as a liquid membrane and the acceptor solution. The extraction parameters affecting the enrichment factor of NR, such as pH, extraction time, elution time, stirring rate, and the voltage were optimized. In addition, UV–Visible (UV–Vis) or electrochemiluminescence spectra were also determined. The extraction rate and capacity of NR could be improved significantly by cathodic polarization. Under the optimized extraction conditions (organic liquid microextraction phase [C₈mim][PF₆], pH 7, stirring rate 300 rpm, extraction time 20 minutes, ultrasonic-assisted elution time 3 minutes, voltage −70 V), the detection limit of 0.38 μg/L and linear correlation coefficient of r > 0.99 were obtained. The established method was successfully applied to the analysis of three soft drink samples, which were spiked with NR standards at the concentrations of 0.1, 1.0, and 5.0 mg/L, and satisfactory results were obtained.     

联合检测血清肿瘤标记物对消化道五种恶性肿瘤的诊断价值

目的检测血清CA72-4、CEA、AFP、CA-50、CA19-9含量水平,评价这五项肿瘤标记物对消化系统五种恶性肿瘤的诊断价值。方法采用电化学发光免疫分析法测定经临床确诊的消化系统恶性肿瘤患者、消化道良性疾病患者和正常人血清CA72-4、CEA、AFP、CA-50、CA19-9含量。结果五项肿瘤标志物在消化道五种恶性肿瘤患者中的含量均显著高于健康人群（P〈0.05）。CA-50和CA19-9在胆囊癌和胰腺癌两种恶性肿瘤与五项指标联合检测的检出率无显著差异（P〉0.05）。CEA在直结肠癌与五项标志物联合检测的检出率也没有显著差异（P〉0.05）。结论不同的消化道恶性肿瘤引起血清CA72-4、CEA、AFP、CA-50、CA19-9含量水平不同。因此,针对不同的消化道恶性肿瘤应采用不同的指标联合检测。     

ECLIA与RIA测定血清C肽结果差异分析

目的:探讨ECLIA与RIA测定血清C肽结果出现差异的原因。方法:采用ECLIA和RIA测定40例健康体检者及8例门诊病人空腹及餐后2小时血清C肽的含量;统一C肽标准品后,用这两种方法测定上述96份血清C肽。结果:当标准品不同时,两法测定结果差异有统计学意义(P<0.05),但相关性良好;当统一标准品后,两法测定结果差异无统计学意义(P>0.05),并具有良好的相关性。结论:对于血清C肽的测定,ECLIA与RIA方法学没有差异,只要标准品统一,两法结果可比,且具有高度相关性。