Quels types de produits céréaliers pour le petit déjeuner ?

Breakfast is an essential meal since it provides 25% of the energetic needs and participates to a better food balance over the day; furthermore breakfast consumption is correlated with a better mental and physical health. An increased consumption of high-quality cereals, such as bread and ready-to-eat cereals, at breakfast should allow reaching the objective of 55% energy supplied by carbohydrates, notably complex carbohydrates (starch and fibre). However, both products exhibit inconstant nutritional qualities according to the raw materials and technological processes used. Brown bread should be favoured instead of white bread which has a low nutritional density and a high glycaemic index; baking processes will have to be improved, and a better communication will be necessary to favour the consumption of breads of high nutritional quality. Ready-to-eat cereals intended for adults are of better nutritional quality than those intended for children, containing less simple sugars and lipids, and more fibre.     

难治性肺部感染的细菌学分析

目的分析难治性细菌性肺部感染的病原菌和药敏试验. 方法对1999年10月～2002年6月间,难治性细菌性肺部感染患者,采用经纤维支气管镜单套管防污染毛刷(PSB),采集下呼吸道标本进行半定量培养和药敏试验. 结果共培养细菌86株,其中革兰阴性杆菌(GNB)66株(76.7%),革兰阳性球菌(GPC)16株(18.6%),主要是铜绿假单胞菌、恶臭假单胞菌、嗜麦芽寡养单胞菌和金黄色葡萄球菌,病原菌存在广泛耐药性. 结论引起难治性肺部感染的病原菌以耐药假单胞菌属和金黄色葡萄球菌为主,应重视病原学诊断.     

bcl-2基因siRNA-2提高急性原代白血病细胞对阿糖胞苷敏感性

目的:研究以bcl-2基因为靶标有效si RNA-2(small interference RNA)能否提高急性原代白血病细胞对阿糖胞苷(Ara-C)敏感性。方法:将si RNA转入原代白血病细胞并与Ara-C联合培养,于24、48、72h,用锥虫蓝拒染法计数活细胞,用免疫细胞化学技术检测原代白血病细胞bcl-2和p53蛋白的表达。结果:si RNA-2(1μmol/L)与Ara-C组(0.2μmol/L)联合作用,在72h内能显著降低原代白血病细胞存活,细胞数从3×10~8/L下降到1.2×10~8/L;显著抑制bcl-2和p53蛋白的表达,与对照组相比bcl-2蛋白表达率下降18.46%;p53蛋白表达率下降14.56%。结论:si RNA-2能提高原代白血病细胞对阿糖胞苷敏感性。     

慢性前列腺炎330例病原检测及药敏分析

目的探讨慢性前列腺炎患者前列腺液中分离的细菌菌谱分布及支原体感染情况，通过药敏试验提供临床用药依据。方法对临床采集的慢性前列腺炎患者前列腺液标本进行培养、鉴定和药物敏感试验，并对所得结果进行统计分析。结果330例患者的前列腺液标本中，培养出革兰阳性球菌116株，革兰阴性杆菌5株，阴道加德纳菌15株，培养出解脲支原体99株，并对各种抗菌药物表现出不同程度的耐药。结论慢性前列腺炎很大部分由细菌及支原体感染引起。     

RASSF1A基因对肝癌细胞化疗药物敏感性的影响

目的探讨RASSF1A基因的表达对肝细胞肝癌化疗药物敏感性的影响。方法利用已构建成功的稳定表达野生型和突变型RASSF1A基因的肝癌细胞株QGY-7703,化疗药物Mitomycin、Adriamycin、Etoposide、5-Fluorouracilur、Cisplatin分别作用各细胞株后,比较生长抑制率、细胞周期及P53、P21、Bax、Caspase-3蛋白的表达水平。结果野生型RASSF1A的表达可提高Mitomycin诱导的肝癌细胞生长抑制率、凋亡发生率（P<0.05）和Caspase-3的活性,对P53、P21、Bax基因的蛋白表达水平没有影响。结论野生型RASSF1A基因可提高肝癌细胞对Mitomycin的敏感性。