顺糖氨铂(Ⅱ)的合成

以氯铂酸钾为原料，经硫酸氨铂(Ⅱ)中间体制得抗肿瘤药物顺糖氨铂(Ⅱ)，操作简便，总收率46．1％。     

二乙基二硫代氨基甲酸钠驱镍作用的实验研究

本文用大鼠研究了带-SH基的二乙基二硫代氨基甲酸钠(Dithiocarb,简称Na-DDC或称铜试剂)和不带-SH基的新螯合剂(H-73-10)的驱镍作用。结果表明,Na-DDC对羰基镍(Ni(CO)_4〕的驱镍作用明显优于H-73-10;与此相反,H-73-10对硝酸镍〔Ni(NO_3)_2〕染毒大鼠的驱镍作用却明显优于Na-DDC。     

TiN: A Suitable Coating for Defibrillator Electrodes?

The electrochemical behavior of porous TiN coating for defibrillator electrodes was investigated by in vitro studies. A commercially available Ti patch was compared with a Ti patch coated with a layer of porous TiN. Similarly, the influence of the TiN coating on a Pt/Ir intracardial electrode was tested. Porous TiN coatings were made by reactive sputtering on the plain electrodes. Electrode potential measurements were performed during monophasic anodic and cathodic defibrillator shocks delivering energies between 1 J and 49 J. Porous TiN coating of Pt/Ir coils was found suitable to deliver cathodal pulses, while a plain coil remains recommended for anodic shocks. TiN coating was of benefit for conventional Ti patches. Particularly, in the case of anodic shocks, the TiN layer protected the Ti-mesh from immediate oxidation. However, above 70 anodic shocks of 49 J, the TiN coating underwent oxidation to an oxynitride. An estimated 180 anodic shocks of 10 J could be delivered before TiN oxidation became measurable by increased polarization.     

新型麻醉药异丙酚的氧化动力学

采用高效液相色谱法测定了异丙酚在不同温度下及其甲醇 水（Ｖ∶Ｖ＝７０∶３０）混合溶液在不同ｐＨ值下的反应速率常数，并求出了异丙酚氧化反应的活化能，结果表明ｐＨ值与温度对异丙酚的氧化动力学均有影响     

Selective modification at the N-terminal region of human growth hormone that shows antagonistic activity

A new analogue of recombinant human growth hormone (hGH), hGH des(1–6,14) was expressed in Escherichia coli, refolded and purified to homogeneity. The mutation decreased the hormone's ability to bind lactogenic and somatogenic receptors through its site 1, and almost completely abolished its ability to bind these receptors through site 2, as evidenced by both binding and gel-filtration experiments. More specifically, the binding to prolactin receptors (PRLRs) from various species or their soluble recombinant extracellular domains (ECDs) was decreased 1.5–4-fold, whereas the binding to hGH receptor (hGHR) was decreased 10–85-fold. These changes caused an almost total loss of hormone agonistic activity in several in vitro bioassays and subsequently, the hGH des(1–6,14) analogue acquired antagonistic properties. This antagonistic activity was dependent upon modification of site 1. In those cases in which the binding was reduced only slightly, e.g. binding to rabbit PRLRs, hGH des(1–6,14) acted as a strong antagonist, whereas in others in which the binding of site 1 was reduced to a higher degree, such as other PRLRs and, in particular, hGHR, the antagonistic activity was correspondingly weaker. Circular dichroism spectra of the analogue suggested that these changes do not result from a decrease in overall -helix content, but rather from minor local structural modifications at the N-terminus.     

PCR检测结核分枝杆菌DNA中不同细菌裂解方法结果观察

目的：寻找适合于临床ＰＣＲ检测结核杆菌ＤＮＡ简单、快速、有效、经济的细菌裂解方法。方法：采用１１种细菌裂解剂对相同数量的结核分枝杆菌进行裂解，裂解上清液直接用于ＰＣＲ扩增，结果：１％ＴｒｉｔｏｎＸ－１００，１％ＮＰ４０，１％Ｔｅｗｅｅｎ２０、和１％ＯＰ四种裂解剂的裂解液可直接扩增出结核分枝杆菌ＤＮＡ；ＳＤＳ，ＮａＯＨ，十二烷基肌酸钠等裂解剂的裂解液直接用于ＰＣＲ扩增均为阴性。结论：１％的Ｔｒｉｔｏｎｘ－１００，ＮＰ４０、Ｔｅｗｅｅｎ２０和乳化剂ＯＰ裂解细菌效果好，又不抑制ＰＣＲ反应，适合于作为临床ＰＣＲ检测结核杆菌的裂解剂。     

长效重组人超氧化物歧化酶的研制及其辐射防护作用

选用无毒、无免疫原性的高分子化合物聚乙二醇(PEG)为修饰剂,以活性酯法制备出PEG- 重组人超氧化物歧化酶(PEG-rh SOD)共价结合物.修饰酶的生物半衰期为15h,比修饰前延长了90倍,对酸、碱、热的耐受力明显高于修饰前.用照射小鼠的活存率评价了PEG-rhSOD的辐射防护作用.小鼠受8.5Gy γ射线照射前1h腹腔注射30万U/kg PEG-rh SOD,可提高活存率42%,与未修饰的rhSOD相比,活存率提高14%.实验仍在进行中.     

Differential effect of pancuronium at the adductor pollicis, the first dorsal interosseous and the hypothenar muscles. An electromyographic and mechanomyographic dose-response study

Cumulative dose-response curves were constructed from evoked compound electromyographic (EMG) recordings in man to compare the sensitivity to pancuronium of the adductor pollicis, the hypothenar and the first dorsal interosseous muscles. Also, the EMG and mechanomyography-based sensitivity of the adductor pollicis muscle were compared. The EMG and the mechanomyogram were evaluated in random sequence in each of 21 adult thiopental, fentanyl and diazepam anesthetized patients. The EMG-based ED50 were 36-38 micrograms.kg-1 with no differences between muscles. The EMG-based ED90 of the adductor pollicis and the hypothenar muscles were 62-65 micrograms.kg-1 compared to the 60 micrograms.kg-1 of the first dorsal interosseous muscle (P < 0.05). ED50 (34 micrograms.kg-1), and ED90 (56 micrograms.kg-1) obtained from the adductor pollicis mechanomyogram were significantly lower than those based on the EMG (P < 0.05). It is concluded that differences in sensitivity to pancuronium exist between the three muscles when evaluated from the EMG, and that the apparent sensitivity of a given muscle to a muscle relaxant may depend upon whether the response is evaluated using EMG or mechanomyography.     

流态化化学气相淀积制备Al2O3—SnO2复合粒子的研究

利用流态化化学气相淀积（ＣＶＤ）制备Ａｌ＿２Ｏ＿３－ＳｎＯ＿２复合粒子。探讨了ＳｎＯ＿２在Ａｌ＿２Ｏ＿３超细颗粒上的包敷状态，考察了反应温度、反应物进料浓度比、反应时间等对反应结果的影响。结果表明，ＳｎＯ＿２在复合粒子团聚体体相中呈均匀分布，形成Ａｌ＿２Ｏ＿３－ＳｎＯ＿２复合粒子，ＳｎＯ＿２均匀淀积在Ａｌ＿２Ｏ＿３超细颗粒原生粒子表面，流态化ＣＶＤ包敷效果优于非流化过程；随反应时间的延长，产物中ＳｎＯ＿２含量线性递增；而当反应温度高于３００℃，反应物Ｈ＿２Ｏ和ＳｎＣｌ＿４配比大于４：１时，ＳｎＯ＿２含量基本保持不变。