Comparing the efficacy of different intra-articular injections for knee osteoarthritis: A network analysis

Background:The findings on the effectiveness of platelet-rich plasma, ozone, and hyaluronic acid in the treatment of osteoarthritis of the knee are controversial, and the existing original studies and meta-analyses are mostly comparisons of a single joint cavity injection method, lacking direct and indirect comparisons of different drugs in the joint cavity. The lack of direct and indirect comparisons of different drugs in the joint cavity makes it difficult to have a clearer and more comprehensive understanding of joint cavity injection methods. In this study, the efficacy of platelet-rich, ozone, sodium hyaluronate, and combined knee cavity injections were compared directly or indirectly using a reticulated meta-analysis in this field, and the efficacy of treatment measures was ranked to provide more comprehensive and reliable evidence-based clinical evidence for the selection of knee cavity injections in osteoarthritis of the knee.Objective:To compare the effects of platelet-rich plasma, ozone, and sodium glassate injection interventions on the efficacy of osteoarthritis of the knee through reticulated Meta-analysis, and to comprehensively compare the clinical effectiveness of platelet-rich plasma, ozone, and sodium glassate injection joint cavity injection for the treatment of osteoarthritis of the knee.Methods:The PubMed, CBM, CNKI, VIP, and Wan-Fang databases were searched for information on the effectiveness of platelet-rich plasma, ozone, and sodium vitrate injection for the comparative treatment of osteoarthritis of the knee, with a search time frame of each database from the date of creation to July 20, 2021. Two investigators independently screened the literature, extracted data according to inclusion and exclusion criteria, and evaluated the quality of the literature in parallel. Statistical analysis was performed using Stata 16.0 software to compare the differences in the efficacy of each treatment measure using the ratio and 95% confidence interval as effect indicators and to rank the efficacy.Results:Thirty-three RCTs with 7003 patients with osteoarthritis of the knee were included, involving 5 therapeutic measures. Meta-analysis showed that the efficacy of platelet-rich plasma injection was superior to both ozone and hyaluronic acid therapies. Hyaluronic acid+ozone and platelet-rich plasma+hyaluronic acid were both superior to ozone and hyaluronic acid monotherapy. The differences in efficacy between hyaluronic acid and ozone compared with platelet-rich plasma were statistically significant, and the differences in efficacy between the 2 combination therapies (platelet-rich plasma+hyaluronic acid, hyaluronic acid+ozone) and the 3 monotherapies (platelet-rich plasma, ozone, hyaluronic acid) were statistically significant. Platelet-rich plasma+hyaluronic acid, hyaluronic acid+ozone compared with 3 monotherapies (platelet-rich plasma, ozone, hyaluronic acid) were statistically significant, except for the difference in efficacy with platelet-rich plasma, which was not statistically significant, indicating that this platelet-rich plasma+hyaluronic acid and Hyaluronic acid+ozone combination therapy was superior to monotherapy. Also, the efficacy of platelet-rich plasma was better than hyaluronic acid and ozone and the difference was statistically significant, indicating that platelet-rich plasma was more effective than ozone and sodium glass in the treatment of osteoarthritis of the knee in monotherapy.Conclusion:It is believed that in the course of clinical practice, hyaluronic acid+ozone or platelet-rich plasma+hyaluronic acid combination therapy or platelet-rich plasma therapy can be preferred for patients with osteoarthritis of the knee.     

利用网络防治精神分裂症复发对照研究

目的：探讨网络对非急性期精神分裂症患者的作用。方法：选取71例非急性期精神分裂症患者随机分为两组,数字化网络组（数字化网络配合社区防治网）35例,社区防治组36例（由社区防治网随访）。最后完成62例。在药物治疗的基础上,对数字化网络组患者进行网上心理咨询,社区防治组进行一般康复干预。随访1年。应用阳性与阴性症状量表（PANSS）、社会功能缺陷筛选量表（SDSS）进行评估,同时监测其复发率和药物依从性。结果：随访结束时PANSS各量表的评分数字化网络组均显著优于社区防治组（P〈0.01）。数字化网络组1年复发恶化率低于社区防治组（P〈0.05）。药物依从性和社会功能数字化网络组均显著优于社区防治组（P均〈0.01）。结论：在药物治疗的基础上,利用数字化网络配合社区防治网对精神分裂症患者预防复发,改善社会功能有明显作用。     

基于变尺度符号化补偿传递熵的EEG压力情感分析

基于脑电信号的情感识别,对于相关情感疾病的诊断与治疗有着重要的临床和科研意义.如何有效地提取特征,提高识别率,减少计算时间是研究的重点.从研究脑通道间定向信息交互的角度出发,结合对瞬时因果效应的补偿算法,提出以变尺度符号化补偿传递熵(VSSCTE)为特征的情感分析方法,并以此构建情感因效性脑网络,使用网络测度与Reli...     

基于网络药理学联合GEO芯片分析百合乌药汤治疗胃癌的分子靶点及作用机制

目的:基于网络药理学联合GEO芯片差异基因分析的方法,分析百合乌药汤治疗胃癌的分子靶点和作用机制.方法:在TCMSP数据库检索中药药物信息,获得百合乌药汤的活性成分和分子靶点.在GEO芯片数据库通过检索“gastric cancer”分析筛选后获得GSE118916芯片数据,通过R软件分析得出差异基因,并绘制热图和火山...     

Vitamin-D Deficiency and Supplementation Altered the Network of the Coronary Arteries in a Rodent Model—In Situ Video Microscopic Technique

The aim of our study was to identify whether vitamin-D deficiency (VDD) can alter the geometry of the coronary-resistance-artery system. Male Wistar rats were divided into vitamin-D-deficient (VD−, n = 10) and vitamin-D-supplemented (VD+, n = 8) groups. After eight weeks, branches and segments of the left-anterior-descending-coronary-artery (LAD) network were analyzed by a video-microscopy technique. Segments were divided into 50 μm-long cylindrical ring units. VDD did not increase the number of morphological abnormalities. The number of segments did not differ between the groups (VD−: 210 and VD+: 224; pooled data of 8 networks). A larger lumen area of branches was found in VD+ group, while 1–4-order branches were lengthier in the VD− group. VD− rats had less rich coronary-resistance-artery networks in terms of 50 µm-long units. (VD−: 6365 vs. VD+: 6602; pooled data of 8 networks). VD+ animals were richer in the 100–350 µm outer diameter range, and VD− animals were richer in the 400–550 µm-diameter units. In VD− rats, 150–200 and 300 µm units were almost missing at higher flow distances from the orifice. Serum vitamin-D alterations caused by dietary changes can affect the geometry of the coronary-artery network, which may contribute to vitamin-D-dependent changes in cardiovascular mortality.     

视觉对人体姿势控制影响的脑功能网络连接机制

目的 研究视觉对人体姿势控制影响及其脑功能网络连接机制。方法 以15名健康青年为研究对象,要求受试者分别进行30 s睁眼、闭眼的双腿站立平衡,采集平衡过程中身体压力中心(center of pressure, COP)和脑电。对COP进行样本熵(SampleEn)计算;对脑电θ、α和β频段,计算相位滞后指数(phase lag index, PLI)构建大脑功能网络,并基于图论计算集聚系数(C)、特征路径长度(L)及小世界网络属性(σ)。结果 人体在双腿站立平衡过程中,闭眼COP_Y样本熵显著高于睁眼(P<0.05)。闭眼α频段PLI平均值显著高于睁眼(P<0.05);闭眼α频段C、σ显著高于睁眼,L显著低于睁眼(P<0.05)。闭眼时α频段额区-中央区-顶区之间的网络连接以及中央区和顶区内连接强度显著高于睁眼(P<0.05)。闭眼时α频段PLI平均值以及C值与COP_Y样本熵中度呈中度负相关(P<0.05)。睁眼时左前额区、左顶区、左枕区α频段PLI平均值与COP_Y样本熵呈中度负相关;闭眼时左...     

大鼠肝再生中环状RNA的表达变化及其对细胞增殖的调节作用

目的 探讨环状RNA(circRNA)在大鼠肝再生中的表达变化及其对细胞增殖的调节作用.方法 用生物高通量检测方法分析114只2/3肝切除大鼠诱导的大鼠肝再生中circRNA的表达变化,用miRanda和TargetScan网站分析大鼠肝再生的靶微小RNA(miRNA)及其mRNA,用基因本体论(GO)和IPA软件分析...     

注意缺陷多动障碍儿童及其父母攻击行为相互作用的网络分析

目的 应用网络分析的方法探讨注意缺陷多动障碍(ADHD)儿童攻击性以及父母攻击性的相互影响。方法 2019年1—12月选择符合美国《精神障碍诊断与统计手册(第5版)》(DSM-5)诊断标准的ADHD儿童108人为ADHD组,正常儿童110人为对照组,两组儿童及其父母均完成攻击问卷。通过构建网络的方法对ADHD及对照组儿童攻击性行为与其父母的攻击性行为之间的相关性进行研究。结果 ADHD组儿童躯体攻击性、言语攻击性、易怒得分高于对照组儿童,差异有统计学意义(t=5.03、2.94、5.92,P<0.001)。ADHD父母的攻击性显著高于对照组,其分量表显示ADHD组父母易怒(t=3.83、3.70)、父母敌意(t=2.69、3.06)均高于对照组,差异有统计学意义(P<0.05)。相关分析显示父母的攻击性和ADHD儿童的攻击性有显著相关(P<0.01);网络分析显示ADHD儿童与正常对照组儿童存在不同的攻击性网络结构,其网络全局强度高于对照组(Z=2.06,P<0.05)。结论 ADHD儿童和其父母较正常儿童均具有较高水平的攻击行为,父母的攻击性和ADHD儿童的攻...     

基于转录组学膀胱癌临床预后模型的构建

目的：筛选膀胱癌预后相关基因，建立膀胱癌预后评分模型。方法：通过UCSC Xena平台下载癌症基因组图谱（TCGA）数据库、基因型和基因表达量关联数据库（GTEx）中406例膀胱癌患者的临床信息和膀胱癌组织RNA测序数据，以及28名健康对照者正常膀胱组织RNA测序数据。采用加权基因共表达网络分析（WGCNA）、单因素Cox回归分析、LASSO回归分析和多因素Cox回归分析筛选膀胱癌预后相关基因并建立预后模型，结合Kaplan-Meier生存曲线、受试者操作特征曲线（ROC曲线）验证模型的准确性。结果：分析得到膀胱癌相关差异表达基因共2308个。WGCNA拟合得到6个基因模块，筛选出对膀胱癌预后有显著作用的基因829个。运用单因素Cox回归与LASSO回归分析筛选出24个与膀胱癌患者预后相关的基因，多因素Cox回归分析训练集数据得到9个作为独立预测因子的基因，分别是 ADCY9、 MAFG_DT、 EMP1、 CAST、 PCOLCE2、 LTBP1、 CSPG4、 NXPH4、 SLC1A6，以此建立膀胱癌患者预后预测模型。训练集中高风险组和低风险组3年存活率分别为31.814%和59.821%，测试集中高风险组和低风险组3年存活率分别为32.745%和68.932%，模型预测训练集和测试集患者预后的ROC曲线下面积均在0.7以上。 结论：本研究建立的模型对膀胱癌高风险和低风险人群的生存情况具有较好的预测能力。     

医疗机构中药制剂创新和持续发展能力评价研究

目的 对医疗机构中药制剂创新和持续发展能力进行综合评价,为行政管理部门决策及各医院中药制剂可持续发展提供参考。方法 以浙江省133家医疗机构中药制剂调研数据为对象,利用层次分析法构建医疗机构中药制剂创新和持续发展能力评价体系,通过反向传播神经网络构建中药制剂室/中心评价体系预测模型。结果 构建的医疗机构中药制剂创新和持续发展能力评价指标体系包含4项二级指标以及19项三级指标,其中研发品种数、超百万品种数、注册品种数、研发方式、制剂室面积指标的权重值较高,分别为 0.15758、0.12928、0.09343、0.07879和0.07458。熵权法结合层次分析法的指标权重构建反向传播神经网络,Levenberg-Marquardt算法和Scaled Conjugate Gradient算法训练模型总体相关系数分别为0.98983和0.9348。结论 本研究构建了一个科学有效的医疗机构中药制剂创新和持续发展能力综合评价体系,可实现对医疗机构中药制剂综合能力的预测评价。