蛇床子水溶性提取物抗诱变性的试验研究

本文报告了蛇床子水溶性提取物,通过Ames、离体细胞的姐妹染色单体互换及活体小鼠骨髓细胞染色体畸变和多染红细胞微核试验,表明了它有较强的抗诱变作用。     

蛇床子总香豆素对成骨细胞产生NO，IL-1及IL-6的影响

 目的以新生大鼠颅盖骨成骨细胞为模型，观察蛇床子总香豆素对成骨细胞产生NO，IL-1和IL-6的影响。方法原代培养新生大鼠颅盖骨成骨细胞，³H-胸腺嘧啶掺入法测定成骨细胞的增殖；Griess 试剂法、小鼠胸腺细胞增殖法和用B9细胞作为靶细胞的生物测定法，分别检测与蛇床子总香豆素共育的无刺激或受LPS及TNF等刺激的成骨细胞培养上清液中NO，IL-1和IL-6的含量。结果蛇床子总香豆素在0.025～2.5 mg·L_-1的范围内抑制成骨细胞自发地或在LPS及细胞因子刺激下产生NO，IL-1及IL-6。结论蛇床子总香豆素的抗骨质疏松作用与其抑制成骨细胞产生NO，IL-1和IL-6的分泌而调节成骨细胞的功能有关。     

蛇床子水提取液对小鼠S180肉瘤的抑制作用

目的:研究蛇床子水提取液的体内抗肿瘤作用.方法:通过S180肉瘤移植建立荷瘤小鼠模型,然后对S180荷瘤小鼠给予4种剂量蛇床子水提取液后观察瘤重、小鼠生存天数及血清涎酸(SA)等指标.结果:剂量组0.04 mg/(g*bw*d)、0.21 mg/(g*bw*d)、0.42 mg/(g*bw*d)平均瘤重低于肿瘤对照组(P＜0.05),且能延长小鼠生存天数(P＜0.01).抑瘤率依次为34.2%、63.6%和33.2%,动物生命延长率依次为53.0%、58.3%和47.0%.结论:蛇床子水提取液具有较强的抗肿瘤作用,有很好的利用前景,值得对其进行深入研究.     

中药蛇床子水溶性提取物中化学成分的抗诱变性研究

本文采用Ａｍｅｓ、体内试验小鼠骨髓细胞染色体畸变和骨髓多染红细胞微核试验,对蛇床子水溶性提取物中９种化学成分（蛇床子素,佛手柑内酯,异虎耳草素、欧芹属素乙、花椒毒酚,花椒毒素,甲基嘧啶,尿嘧啶和１种待定化合物）进行了抗诱变性研究。结果表明,蛇床子素,佛手柑内酯,异虎耳草素,欧芹属素乙,花椒毒酚和花椒毒素在典曲霉菌素Ｂ１诱变性的抑制作用中具有较高活性。蛇床子素,佛手柑内酯、异虎耳草素和欧芹属素乙在环     

Effects of osthole on osteoporotic rats: a systematic review and meta-analysis

ContextCnidium monnieri Cusson (Apiaceae) has been used in traditional Asian medicine for thousands of years. Recent studies showed its active compound, osthole, had a good effect on osteoporosis. But there was no comprehensive analysis.ObjectiveThis meta-analysis evaluates the effects of osthole on osteoporotic rats and provides a basis for future clinical studies.MethodsChinese and English language databases (e.g., PubMed, Web of Science, Cochrane Library, Google Scholar, Embase, China National Knowledge Infrastructure, Wanfang Data Knowledge Service Platform, Weipu Chinese Sci-tech periodical full-text database, and Chinese BioMedical Literature Database) were searched from their establishment to February 2021. The effects of osthole on bone mineral density, osteoclast proliferation, and bone metabolism markers were compared with the effects of control treatments.ResultsTo our knowledge, this is the first meta-analysis to evaluate osthole for the treatment of osteoporosis in rats. We included 13 randomized controlled studies conducted on osteoporotic rats. Osthole increased bone mineral density (standardized mean difference [SMD] = 3.08, 95% confidence interval [CI] = 2.08–4.09), the subgroup analysis showed that BMD significantly increased among rats in osthole <10 mg/kg/day and duration of osthole treatment >2 months. Osthole improved histomorphometric parameters and biomechanical parameters, also inhibited osteoclast proliferation and bone metabolism.ConclusionsOsthole is an effective treatment for osteoporosis. It can promote bone formation and inhibit bone absorption.     

蛇床愈伤组织形态发生过程中过氧化物酶的变化规律研究

目的:研究蛇床愈伤组织形态发生过程中过氧化物酶活性的变化与过氧化物酶同工酶的差异。方法:采用分光光度法测定过氧化物酶活性,采用聚丙烯酰胺凝胶平板电泳分析过氧化物同工酶。结果:已分化愈伤组织的酶活性高于未分化愈伤组织,未分化愈伤组织、产生胚状体的愈伤组织及分化芽和根的愈伤组织的酶活性成线性递增关系。不同分化类型愈伤组织的过氧化物酶同工酶谱有明显的差异。三类愈伤组织都有酶带1和2,分化的愈伤组织都有3和5,分化芽和根的愈伤组织还有酶带4,分化芽和根的愈伤组织的酶带3比形成胚状体的愈伤组织的酶带3要宽,着色也深。结论:过氧化物酶活性与愈伤组织分化程度呈正相关;酶带1和2显示的两种同工酶与愈伤组织的增殖有关,酶带3和5是与分化有关的特异性蛋白质。酶带4可能催化器官发生特有反应,且对过氧化物酶的活性影响较大。