Expression of Opa interacting protein 5 (OIP5) is associated with tumor stage and prognosis of clear cell renal cell carcinoma

Opa interacting protein 5 (OIP5), overexpressed in some types of human cancers, has been reported to be associated with the carcinogenesis of human cancer. However, the biological function and clinical significance of OIP5 in human Clear Cell Renal Cell Carcinoma (CCRCC) remains unknown. In the present study, we found the expression of OIP5 was markedly upregulated in surgical CCRCC specimens and CCRCC cell lines. Immunohistochemical analysis revealed that paraffin-embedded archival CCRCC specimens exhibited higher levels of OIP5 expression than normal renal tissues. Further statistical analysis suggested the upregulation of OIP5 was positively correlated with the Fuhrman grade (P = 0.02), T classification (P = 0.015), N classification (P = 0.018) and clinical stage (P = 0.035). Also, patients with high OIP5 expression dramatically exhibited shorter survival time (P = 0.001). In addition, the OIP5 expression was an independent prognostic marker of overall survival of CCRCC patients in a multivariate analysis (P = 0.008). Experimentally, we demonstrated that silencing OIP5 in CCRCC cell lines by specific siRNA clearly inhibited cell growth. In conclusion, our findings suggested that OIP5 could be a valuable marker of CCRCC progression and prognosis, and a promising therapeutic target for CCRCC.     

Effects of Invisalign (G5) with virtual bite ramps for skeletal deep overbite malocclusion correction in adults

ObjectivesTo investigate the skeletal and dentoalveolar effects of Invisalign''s G5 protocol with virtual bite ramps in the treatment of adults with skeletal deep bites.Materials and MethodsThis retrospective study was conducted on consecutively treated adults presenting with skeletal deep bites as defined by the Overbite Depth Indicator (ODI). Subjects were divided into 2 groups: Invisalign group (n = 24) treated with the Invisalign G5 protocol and a full fixed appliance (FFA) group (n = 24) treated with edgewise FFAs and matched to the Invisalign group by ODI, sex, type of malocclusion, and non-extraction treatment. Pretreatment (T₁) and post–comprehensive treatment (T₂) lateral cephalograms were obtained and analyzed.ResultsBoth the Invisalign and FFA groups showed significant changes from T₁ to T₂ in ODI and other skeletal and dentoalveolar measurements. The mean change in ODI was −1.5° (P < .001) for the Invisalign group and −2.0° (P < .001) for the FFA group. The mean decrease in overbite was 1.3 mm (P < .001) and 2.0 mm (P < .001) for the Invisalign and FFA groups, respectively. The mean increase in mandibular plane angle (Sn-GoGn) was 0.65° (P = .003) for the Invisalign group and 1.15° (P < .001) for the FFA group. When the groups were compared with each other, both ODI (P = .03) and overbite (P = .003) were significantly different in addition to other measurements.ConclusionsAlthough FFA treatment had more apparent skeletal changes for deep bite adult patients when compared with Invisalign, both systems were effective in opening deep bites at dentoalveolar and skeletal levels.     

Orthodontic bone screws: A quick update and its promising future

Orthodontic bone screws (OBSs) provide intraoral anchorage by penetrating oral mucosa and seating firmly in basilar bone (BB). Retromolar (prosthetic-type) implants introduced the extra-alveolar (E-A) concept for BB anchorage to move teeth throughout the alveolar process, but the clinical procedures were complex and expensive. Titanium alloy (Ti) miniscrews placed in inter-radicular (I-R) alveolar bone are more convenient and provide some tooth movement potential, but multiple screws are usually required and the devices often interfere with the path of tooth movement. The advantages of BB anchorage and the convenience of miniscrew are combined into the E-A OBS system. These miniscrews are relatively large in diameter (2 mm), and strong (stainless steel), which are placed intraorally in the BB of the infra-zygomatic crest (IZC) and mandibular buccal shelf (MBS). E-A OBSs provide osseous anchorage to retract the dentition and/or rotate either arch. Recovery of impactions is effectively managed with lever arm springs anchored with IZC or MBS bone screws. An emerging frontier is BB anchorage for correcting severe malocclusions with clear aligners. Since the osseous-anchored mechanics are complementary, fixed appliances and clear aligners can be used individually or in tandem to resolve a broad variety of malocclusions. This report summarizes current concepts for conservatively managing complex malocclusions such as severe crowding, skeletal discrepancies, asymmetries and impactions with the OBS system.     

miRNA-221 promotes proliferation,migration and invasion by targeting TIMP2 in renal cell carcinoma

Introduction: MicroRNAs (miRNAs) play important roles in tumorigenesis. In this study, we investigated the role of miR-221 in the development and progression of clear cell renal cell carcinoma (ccRCC). Methods: Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-221 in ccRCC tissues and cell lines. Then, we investigated the role of miR-221 to determine its potential roles on renal cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was conducted to confirm the target gene of miR-221 and the results were validated in renal cancer cells. Results: In the present study, we found that miR-221 was significantly increased in ccRCC tissues and cell lines. Knocked-down expression of miR-221 remarkably inhibited cell proliferation, migration and invasion of renal cancer cells. Moreover, at the molecular level, our results suggested that TIMP2 as a direct target of miR-221 through which miR-221 promoted tumor cell proliferation, migration and invasion. Conclusions: These findings suggested that miR-221 play an oncogenic role in the renal cancer cell proliferation, migration and invasion by directly inhibiting the tumor suppressor TIMP2, indicating miR-221 act as a potential new therapeutic target for the treatment of ccRCC.     

Prognostic significance of preoperative C-reactive protein: albumin ratio in patients with clear cell renal cell carcinoma

We undertook a retrospective analysis to evaluate the C-reactive protein/albumin (CRP/Alb) ratio for its prognostic value in patients with clear cell renal cell carcinoma (CCRCC). The study comprised 406 CCRCC patients undergoing nephrectomy between 2003 and 2012 in our hospital. The correlations among the pretreatment CRP/Alb ratio, clinicopathological parameters, and overall survival (OS) were evaluated. An elevated CRP/Alb ratio was associated with older age at surgery (P=0.007), more advanced TNM stage (P<0.001), more presence of tumor necrosis (P<0.001) and lymphovascular invasion (P<0.001), lower concentration of hemoglobin (P<0.001) and calcium (P=0.005), and shorter OS (P<0.001). The multivariate analysis confirmed that the CRP/Alb ratio independently predicted the OS of patients with CCRCC (P<0.001), the Glasgow Prognostic Score (GPS) (P=0.001) and modified GPS (mGPS) (P=0.019) were independent prognostic factors also. At last, we evaluated the prognostic value of the CRP/Alb ratio compared with the similar inflammation-based prognostic scores GPS and mGPS using the area under the curve (AUC). Although the differences were not statistically significant, the AUC value of the CRP/Alb ratio (continuous, categorical) was higher compared with the GPS and mGPS, except that the AUC value for the CRP/Alb ratio (categorical) at 3 years was lower than that for the GPS. The CRP/Alb ratio could take the place of the GPS and mGPS in terms of predicting prognosis in CCRCC.     

Expression of PGAM1 in renal clear cell carcinoma and its clinical significance

Objective: This study is aimed to evaluate the expression of phosphoglycerate mutase 1 (PGAM1) in normal kidney and clear cell renal cell carcinoma (CCRCC), also to evaluate the correlation between PGAM1 expression and clinicopathological features in CCRCC. Methods: PGAM1 expression was detected in 80 cases of normal kidney and 192 cases of CCRCC by immunohistochemistry (IHC). Meanwhile, PGAM1 expression measured in 8 cases of CCRCC and matched normal kidney tissues by Western blot. Then, the correlation between PGAM1 expression and clinicalpathological features was analyzed in CCRCC. Results: IHC results exhibited that the high-expression rate of PGAM1 in CCRCC tissues was 45.8%, which was significantly higher than those in normal kidney tissues (32.5%, P=0.044). Meanwhile, PGAM1 expression in CCRCC was significantly greater compared with those in normal kidney by Western blot. Moreover, PGAM1 expression was significantly associated with age, tumor size and T stage in CCRCC. Conclusion: PGAM1 is highly expressed in CCRCC and correlated with clinicalpathological features, which may contribute to tumor formation and progression.     

清晰肝切除

出血仍是肝脏外科首先需要面对和解决的问题,它直接决定手术的成败和患者的生命,同时决定外科医生的荣辱和自信;胃肠外科早已进入清晰时代,作为"同室兄弟"的肝脏外科也应跨入清晰时代;"清晰"是精细外科的基本要求,也是精准肝切除的前提。为此,笔者提出清晰肝切除的概念,并结合自己的临床实践探讨如何实现清晰肝切除。     

青少年隐形保持器的适应性和依从性与人格特征的调查

目的:探讨青少年使用隐形保持器的适应性和依从性,及其与人格特征的关系.方法:纳入青少年正畸患者50例,在拆除固定矫治器后以透明压膜式隐形保持器保持.在隐形保持器戴用2个月后,问卷调查患者对隐形保持器的适应性及依从性.以艾森克人格问卷评估患者的人格特征.采用SAS8.0软件包中的Spearman秩相关评估患者隐形保持器适应性和依从性与人格特征的关系.结果:42例患者完成问卷.76％的患者反映隐形保持器无黏膜刺激,95％反映其不影响日常活动.但75％反映隐形保持器有异物感,71％反映影响发音.另外,40％的患者戴用时间不足18h,31％曾遗落保持器,83％不能做到进食后刷牙.患者的人格特征中情绪不稳定性越高,保持器对咀嚼的影响越大,相关系数为0.32;精神质越强,保持器异物感和对面部肌肉酸痛的影响越明显,相关系数分别为0.31和0.35.但精神质越强者,保持器对情绪的影响越小,相关系数为-0.34;越外向的患者越常发生保持器损坏,相关系数为0.31.结论:青少年隐形保持器的适应性较好,但依从性不佳.隐形保持器的适应性和依从性与患者人格特征有关.提示青少年患者选用隐形矫治器时应重视患者依从性,可以通过人格特征预测.     

无托槽隐形矫治器整体内收上颌前牙的三维有限元分析

目的:研究无托槽隐形矫治器整体内收上颌前牙过程中上颌前牙所受的应力情况及初始移动规律.方法:采用CBCT扫描已拔除双侧上颌第一前磨牙患者,建立上牙列、牙周膜及牙槽骨的初始复合体模型.激光扫描患者牙冠外形并与初始模型三维重叠建立终模型.应用ANSYS Workbench软件分析安装无托槽隐形矫治器时上颌前牙的应力分布及初始位移趋势.结果:建立了具有高仿真度的上颌复合体三维有限元模型;上颌双侧中切牙及侧切牙初始位移趋势一致,表现为远中舌向倾斜移动,且均有伸长趋势,其牙周膜应力分布与其位移趋势相一致;上颌双侧尖牙表现为远中倾斜移动趋势.结论:无托槽隐形矫治器在整体内收上颌前牙时,上颌前牙均表现为倾斜移动,且有伸长趋势.     

Renal tumors with clear cells. A review

The spectrum of primary renal tumors in which clear cells may appear is revisited in this review. The pathologist's viewpoint of this topic is pertinent because not all the tumors with clear cells are carcinomas and not all renal cell carcinomas with clear cells are clear cell renal cell carcinomas. In fact, some of them are distinct entities according to the new WHO classification. The morphological approach is combined with genetics. Renal cell carcinoma related to von Hippel–Lindau disease is reviewed first because many of the genetic disorders underlying this disease are also present in sporadic, conventional renal cell clear cell carcinomas. Subsequently, conventional renal cell clear cell carcinomas, familial, non von Hippel–Lindau-associated renal cell carcinomas, translocation carcinomas, hereditary papillary renal cell carcinomas, carcinomas associated to tuberous sclerosis and to Birt–Hogg–Dubé syndrome, chromophobe renal cell carcinomas, carcinomas associated with end-stage renal disease, and clear cell tubulopapillary carcinomas are reviewed. Finally, epithelioid angiomyolipoma is also considered in this review.