Correlation between 1.25 dihydroxyvitamin D serum levels and fractional rate of intestinal calcium absorption in hypercalciuric nephrolithiasis. role of phosphate

Summary Different mechanisms could explain the elevated calcium elimination, the main cause of calcium oxalate renal stones. Our results suggest that phosphate levels are decreased in patients with absorptive hypercalciuric nephrolithiasis and elevated serum dihydroxyvitamin D. This could be the reason why in this group of patients oral phosphate treatment prevented hypercalciuria and renal lithiasis.This work was supported by FlSss 89/0799     

Calcium incorporation in cultured chondroblasts perturbed by an electromagnetic field

We tested the hypothesis that electric perturbation influences 45Ca incorporation in extracellular matrix (ECM) of cartilage in vitro. Hypertrophic chondroblasts of tibial epiphyses (HC), sternum (SC), and skin fibroblasts (F) were cultured from chick embryos. HC, SC, and F cells were micromass seeded three times per week and maintained at 37.5 degrees C with 5% CO2 for two weeks. Cultures were randomly designated control (C) or exposed (E) to a pulsed electromagnetic field (PEMF). A time course experiment of calcium incorporation for all cultured groups showed that 24 h of exposure produced the largest biological response in chondroblasts. Calcium incorporation required supplemental phosphate. Autoradiography data indicated that the calcium incorporation into macromolecules largely occurred in the ECM. 45Ca steady-state perturbation was enhanced by Streptomyces hyaluronidase (SH) but not by testicular hyaluronidase (TH). 45Ca incorporation experiments tested the effects of phosphate, SH, TH, and PEMF alone and in various combinations on these cultures. Only PEMF or SH plus PEMF with phosphate enhanced 45Ca incorporation. Other experiments examined the effect of rotenone or freeze-thawing on cells exposed to PEMF. PEMF plus freeze-thaw enhanced calcium incorporation in HC only. PEMF appeared to cause disruption of the ECM, enhancing the probability of matrix calcification.     

钙剂加维生素D治疗对糖尿病患者钙代谢的影响

本文报道NIDDM患者在常规治疗的基础上加用钙剂和维生素D治疗后的钙代谢变化。糖尿病患者单纯用常规治疗控制血糖后,负钙平衡不能完全纠正,而加用钙剂和维生素D治疗后,负钙平衡能完全纠正,并且高于正常对照组平衡值(P<0.01)。说明钙剂加维生素D治疗对糖尿病性骨质疏松是有益的。     

Parvalbumin expression reveals a vibrissa-related pattern in rabbit SI cortex

The expression of parvalbumin-like immunoreactivity (PV-LIR) was examined in the mystacial representation within the primary somatosensory cortex (SI) of postnatal day 21 and adult rabbits. PV-LIR was expressed in a prominent vibrissa-like array of patches in layer IV despite the fact that barrels were indistinct in the cytoarchitecture. Each patch consisted of dense terminal-like PV-LIR and a preferential concentration of intensely labeled stellate neurons. Layer V contained scattered small and large intensely labeled basket cells. Layer Vb had a distinct layer of lightly labeled large pyramidal cells that received labeled basket cell terminations. Upper layer VI also contained patches of terminal-like PV-LIR that were in register with the overlying vibrissae pattern. These patches also contained a preferential distribution of labeled non-pyramidal cells as well as modified pyramidal cells. These results suggest that PV-LIR in rabbits delineates cortical modules composed of thalamorcotical afferents and inhibitory local circuits in the absence of a distinct barrel cytoarchitecure. In contrast, prior studies of rat SI cortex have revealed a distinct barrel cytoarchitecture but a uniform distribution of PV-LIR. The differences in PV-LIR between rodents and lagomorphs within the vibrissae representation in SI may be related to species differences in thalamic and local cortical circuits devoted to the whisker sense.     

人工关节置换充填骨水泥对血液动力学的影响

目的观察人工关节置换术中充填骨水泥对血液动力学的影响。方法40例ASAⅡ～Ⅲ级行人工关节置换术的患者，观察骨水泥充填后10min内血液动力学的变化。结果充填骨水泥后10min内患者血液动力学都有显著变化（P〈0．05），个别患者血压下降严重。需升压药支持。结论人工关节置换术中充填骨水泥可致血液动力学严重波动，应引起重视。     

氯胺酮对荷包牡丹碱诱导PC12细胞内Ca2+浓度波动方式的影响

目的研究氯胺酮对荷包牡丹碱诱导PCI2细胞内Ca^2＋浓度波动方式的影响。方法使用含25ng／LNGF的DMED培养基在多聚赖氨酸包被的培养皿中培养PCl2细胞；与终浓度10gmol／L的Ca^2＋指示剂Fluo-3 AM ester共孵育30min洗涤后，加入终浓度50gmol／L荷包牡丹碱；在激光共聚焦显微镜选定多个细胞分别测定荧光强度的变化；随后加入氯胺酮，记录细胞荧光强度的改变。在试验结束前依次加入Triton X-100和EGTA分别记录单个细胞最大荧光强度（Fmax）和最小荧光强度（Fmin），以计算细胞内Ca^2＋的相对强度。结果氯胺酮不改变荷包牡丹碱诱导PCl2细胞内Ca^2＋浓度波动的基线，但抑制细胞内Ca^2＋浓度升高的幅度（P〈0．05），缩短相邻波峰间的时间间隙（P〈0．05）。结论氯胺酮不仅改变荷包牡丹碱诱导PCl2细胞内Ca^2＋浓度升高的幅度，而且改变Ca^2＋浓度波动的周期。     

Distal radial fractures Injectable calcium phosphate bone cement versus conventional treatment

AMEDLINEsearchwasconductedtoidentifystudiespublishedfromJanuary1999toMarch2004thatcom-paredinjectablecalciumphosphatebone(NorianSRS)cementwithconventionaltreatmentindistalradialfrac-tures.Fromalistof13articlesidentifiedfromthesearchstrategy,fourarticleswe…     

钙剂治疗轻中度妊高征临床应用分析

目的：观察钙剂治疗轻中度妊高征的临床疗效。方法：选择2007年3月-2009年12朋我院产科门诊和住院的轻中度妊高征患者50例,随机分为治疗组和对照组,治疗组给予硝苯地平5-10mg＋碳酸钙、维生素D3合成制剂1片/d;对照组给予硝苯地平5-10mg/次,根据血压变化调整每天硝苯地平用药次数,观察血压、蛋白尿的平均水平,并对结果进行统计学分析。结果：两组患者治疗2周后,平均动脉压、尿蛋白下降,血清钙含量增加,治疗组有效率为92%,对照组为80%,两组疗效比较,差异有统计学意义（P〈0.05）。结论：硝苯地平常用于高血压的治疗,但副作用较大,常需频繁地调整药物剂量,患者尤其妊高征患者对药物的顺应性较差,钙剂几乎没有副作用发生,在联合硝苯地平治疗妊高征的同时为胎儿的发育需要提供了充足的钙源,故应将补充钙剂作为临床轻中度妊高征治疗的首选措施。