五苓散加减治疗中心性浆液性脉络膜视网膜病变临床研究

目的 观察五苓散加减辨证治疗中心性浆液性脉络膜视网膜病变（CSC）的临床疗效。方法 将60例CSC患者随机分为治疗组及对照组各30例。治疗组口服五苓散加减方治疗,对照组口服维生素B1、维生素C治疗。2组均以1月为1个疗程,治疗3个疗程后观察临床疗效。结果 治疗组总有效率为96.7%,对照组总有效率为73.3%,治疗组疗效优于对照组（P<0.05）;2组治疗前后视力、FFA荧光渗漏面积及水肿程度比较,P<0.01,治疗组优于对照组（P<0.05）。结论 五苓散加减方治疗CSC有良好疗效。      

鞣酸对抗TPA和香烟烟雾凝聚物对人白细胞DNA的损伤及其抗氧化作用

从中药五倍子中提取的鞣酸50和100μg/ml可显著地对抗TPA 100ng/ml引起的人白细胞DNA的损伤,30和50μg/ml可有效地对抗香烟烟雾凝聚物(CSC)400μg/ml引起的人白细胞DNA断链;鞣酸5μg/ml可明显对抗VB_2+光产生的O_2~-,但鞣酸浓度需增至80μg/ml才能明显对抗CSC产生的O_2~-。此外,鞣酸5μg/ml可显著对抗H_2O_2引起的红细胞膜脂质过氧化作用,此对抗作用随鞣酸剂量加大而渐明显。     

盆腔浆液性肿瘤患者腹水中侧群(SP)细胞及乙醛脱氢酶阳性(ALDH+)细胞比例高于原发灶

 目的  探讨盆腔浆液性肿瘤干样细胞(cancer stemlike cells,CSC)比例与腹腔转移的关系。方法  2011年7月至2013年7月共采集17例未行化疗的女性盆腔浆液性肿瘤患者的肿瘤原发灶及腹水标本,分别采用Hoechst33342拒染法检测侧群(side population,SP)细胞、乙醛脱氢酶(aldehyde dehydrogenase,ALDH)标记法检测ALDH+细胞,观察两者间SP细胞及ALDH+细胞的表达比例差异,并分析其与盆腹腔转移之间的关系。结果  在可检测的标本中,73.3% (11/15)的患者肿瘤原发灶或腹水中SP细胞检测阳性(SP细胞比例>0.15%),100% (10/10)的患者原发灶或腹水中检测到ALDH+细胞。比较患者不同部位SP细胞和ALDH+细胞比例,可见73.3% (11/15)的患者腹水中SP细胞明显高于原发灶(P=0.01),90%(9/10)的患者腹水中的ALDH+细胞比例高于原发灶(P=0.03),且腹水与原发灶中SP细胞及ALDH+细胞均高的患者与仅SP细胞或ALDH+细胞高的患者发生肿瘤盆腹腔转移的比例差异无统计学意义(P>0.05),但其盆腹腔转移部位数明显较后者多(P<0.05)。结论  盆腔浆液性肿瘤患者腹水中CSC比例明显高于原发灶,推测CSC与肿瘤盆腔种植转移和进展有关。     

心复康联合骨髓间充质干细胞对心脏干细胞CD31和VEGFR2蛋白表达的影响

[目的] 观察心复康联合骨髓间充质干细胞(BMSC)调节心脏干细胞(CSC)内皮细胞标记物CD31(CD31)和血管内皮生长因子受体2(VEGFR2)的蛋白表达。[方法] 原代获取BMSC,制备并鉴定心复康含药血清,酶联免疫吸附法(ELISA法)检测心复康对BMSC分泌基质细胞衍生因子1α(SDF-1α)的影响。组织块贴壁法分离CSC,并行流式细胞分选及免疫荧光鉴定。利用Transwell小室建立细胞共培养体系,实验分为对照组、心复康组、抑制剂组,蛋白质印迹法(Western Blot)分析各组CSC的CD31和VEGFR2蛋白表达情况。[结果] 与另两组相比,心复康组CSC的CD31和VEGFR2蛋白表达显着增高,差异有统计学意义(P<0.05).[结论] BMSC经心复康干预后,可促进CSC的CD31和VEGFR2蛋白表达,提示SDF-1α/CXCR4轴为其作用机制之一。     

Granular cell tumor of the lacrimal sac and nasolacrimal duct: no invasive behavior with incomplete resection

OBJECTIVE: Granular cell tumors are rare tumors of the periorbital region, and we present the second such case in the lacrimal sac. The natural history and clinical behavior of these tumors is reviewed. DESIGN: Case report. METHODS: The clinical presentation, workup, surgical approach, and pathology of a case of granular cell tumor of the lacrimal sac are presented. RESULTS: There has been no recurrence of the lacrimal sac tumor with incomplete resection. CONCLUSIONS: This case history further adds to the understanding of the biologic behavior of granular cell tumors in the periorbital region.     

Chronic psychosocial stress results in sensitization of the HPA axis to acute heterotypic stressors despite a reduction of adrenal in vitro ACTH responsiveness

Although chronic psychosocial stress is often accompanied by changes in basal hypothalamo-pituitary-adrenal (HPA) axis activity, it is vital for a chronically-stressed organism to mount adequate glucocorticoid (GC) responses when exposed to acute challenges. The main aim of the present study was to test whether this is true or not for the chronic subordinate colony housing (CSC, 19 days) paradigm, an established and clinically relevant mouse model of chronic psychosocial stress. As shown previously, CSC mice are characterized by unaffected morning and decreased evening plasma corticosterone (CORT) levels despite enlarged adrenals, suggesting a maladaptive breakdown of adrenal functioning. Plasma CORT levels, determined by repeated blood sampling via jugular vein catheters, as well as relative right adrenal CORT content were increased in CSC compared with single-housed control (SHC) mice in response to acute elevated platform (EPF, 5min) exposure. However, in vitro stimulation of adrenal explants with physiological and pharmacological doses of ACTH revealed an attenuated responsiveness of both the left and right adrenal glands following CSC, despite mRNA and/or protein expression of melanocortin 2 receptor (Mc2r), Mc2r accessory protein (MRAP), and key enzymes of steroidogenesis were not down-regulated. Taken together, we show that chronic psychosocial stressor exposure impairs in vitro ACTH responsiveness of both the left and right adrenal glands, whereas it increases adrenal responsiveness to an acute heterotypic stressor in vivo. This suggests that an additional factor present during acute stressor exposure in vivo rescues left and right adrenal ACTH sensitivity, or itself acts as CORT secretagogue in chronically stressed CSC mice.     

Mucosal immunosuppression and epithelial barrier defects are key events in murine psychosocial stress-induced colitis

Chronic psychosocial stress is a risk factor for many affective and somatic disorders, including inflammatory bowel diseases. In support chronic subordinate colony housing (CSC, 19 days), an established mouse model of chronic psychosocial stress, causes the development of spontaneous colitis. However, the mechanisms underlying the development of such stress-induced colitis are poorly understood. Assessing several functional levels of the colon during the initial stress phase, we show a pronounced adrenal hormone-mediated local immune suppression, paralleled by impaired intestinal barrier functions, resulting in enhanced bacterial load in stool and colonic tissue. Moreover, prolonged treatment with broad-spectrum antibiotics revealed the causal role of these early maladaptations in the development of stress-induced colitis.Together, we demonstrate that translocation of commensal bacteria is crucial in the initiation of stress-induced colonic inflammation. However, aggravation by the immune-modulatory effects of fluctuating levels of adrenal hormones is required to develop this into a full-blown colitis.