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81.
静电法制备小微囊包裹成年猪胰岛的实验研究 总被引:4,自引:0,他引:4
目的 探索用静电法制备小微囊包裹成年猪胰岛细胞。方法 用自制的静电微囊发生装置、制备海藻酸钠 -多聚赖氨酸 -海藻酸钠 (APA)微囊 (微囊直径 <35 0 μm) ,包裹成年猪胰岛 ,体外检测APA小微囊猪胰岛生物活性及微囊膜的通透性 .结果 静电法制备的APA微囊直径 30 0~ 35 0 μm ,大小相对均一 .小微囊包裹成年猪胰岛后 ,每个微囊内可见 1~ 2个胰岛团 ,表面光滑 .囊内胰岛组织学结构完整 ,体外培养微囊化胰岛素分泌良好 ,葡萄糖刺激释放明显 ,显示了良好的细胞活力及微囊膜通透性 .结论 用我们自制的静电微囊发生装置能制备APA微囊包裹成年猪胰岛细胞 ,微囊直径 30 0~ 35 0 μm ,表面光滑 ,囊内猪胰岛生物活性良好 相似文献
82.
利用脉搏波特征参数连续测量血压的方法研究 总被引:11,自引:0,他引:11
通过研究血压与脉搏波特征参数的关系,提出了一个选择多个与血压相关的脉搏波特征参数,通过回归分析建立适合不同人的特征方程,进行血压的连续测量的新方法,为了验证测量结果的准确性;研制了张力计。实验结果表明:该方法具有较高的测量精度,可以应用于临床危重病人的血压监护和一般医学研究中,经过进一步地改进和完善后可应用于载人航天和日常动态环境。 相似文献
83.
超短波治疗慢性肺源性心脏病患者的初步探讨 总被引:1,自引:0,他引:1
目的:探讨超短波对慢性肺心病CCP患者的临床应用价值。方法:急性发作期CCP患者87例分为超短波治疗组45例和对照组42例,治疗组在常规治疗的基础上给予超短波治疗,对照组行常规治疗。治疗前后分别测定两组患者血浆中血管内皮生长因子VEGF、内皮素(ET-1)含量,动脉血氧分压(PaO2)和平均肺动脉压(mPAP)水平。结果:与对照组比较治疗组治疗后PaO2显著升高(P<0.01),VEGF、ET-1、mPAP明显降低(P<0.01)。VEGF、ET-1与PaO2呈负相关(P<0.01)、与mPAP呈正相关(P<0.01)。结论:超短波可以通过降低肺动脉高压对CCP患者起治疗作用。 相似文献
84.
用于电刺激听神经方波刺激器的研制 总被引:4,自引:0,他引:4
本文主要介绍了用于电刺激听神经方波刺激器的基本设计原理 ,组成结构和三个主要性能指标 ,脉宽 相位 :2 5 μs phase、 5 0 μs phase、 10 0 μs phase ;电流强度 :5 0 μA~ 4 0 0 0 μA (每 5 0 μA一档上、下可调 ) ;频率 :30次 秒、 2 0 0次 秒、 4 0 0次 秒、 10 0 0次 秒、 12 0 0次 秒、 2 0 0 0次 秒 ,其特点有人机界面友好 ,性能稳定 ,安全性好 ,无需安装光电藕合器。为研究听神经兴奋性、优化电刺激听神经参数、以及评估电极的有效性和安全性提供实验仪器 相似文献
85.
W. F. Genadry R. E. Kearney I. W. Hunter 《Medical & biological engineering & computing》1988,26(5):489-496
Stochastic system identification techniques were used to determine the dynamic relationship between the electromyogram (EMG)
and torque in the ankle muscles of normal human subjects. EMG and torque were recorded while subjects modulated ankle torque
by tracking a computer-generated stochastic waveform. Nonparametric impulse response functions (IRFs) relating EMG to ankle
torque were computed and parameterised by determining the parameters of the second-order system which provided the best least-squares
fit. Two sets of experiments were carried out. In the first, the mean level of torque was varied from 5 per cent of the maximum
voluntary contraction (MVC) to 30 per cent MVC while the depth of modulation was held constant at ±5 per cent of MVC. In the
second series of experiments the mean torque was held constant at 25 per cent MVC while the depth of modulation was varied
from ±2.5 per cent to ±25 per cent. The major findings were: (1) A second-order, low-pass filter provided a good quasilinear
model of the EMG/force dynamics under all conditions; (2) The model parameters depended only weakly on the mean level of torque;
(3) In contrast, the model parameters depended strongly on the amplitude with which the contraction was modulated; the natural
frequency increased significantly with the depth of modulation. 相似文献
86.
ESWL实际焦点位置的理论和数值分析 总被引:6,自引:1,他引:6
从理论和数值模拟两个方面分析了ESWL中的非线性的动力学焦点和实际焦点的位置 ,给出三种典型的动力学焦点的数值结果 ,说明非线性动力学焦点和实际焦点一般不是几何焦点 ,指出正确确定实际焦点在ESWL技术中的重要性。 相似文献
87.
88.
It has been believed that the primary arterial trunk of the mammalian forelimb is derived from the 7th intersegmental artery.
Here we examined the early morphogenesis of the arteries and nerves in the forelimb region by adopting a method that combined
intravascular dye-injection with nerve staining to whole mounted rat embryos. The study was carried out on greater numbers
of specimens at smaller intervals of embryonic stages and from earlier stages than those in previous reports. We report that:
(1) The multiple primary arterial trunks in the forelimb region (primary subclavians) originate directly from the lateral
surface of the dorsal aorta independently of the intersegmental arteries, previous to the formation of limb buds. (2) The
tips of the 8th (and the 9th) primary subclavians that originate from the aorta near the origin of the 8th (or the 9th) intersegmental
artery bend cranially and/or caudally. With the formation of limb bud, they extend to form the longitudinal trunks in the
presumptive axillary region. The primary arteries in the free arm region branch off from this longitudinal trunk, and one
of them develops into the axial artery. (3) The origins of the primary subclavians shift their positions on the surface of
the dorsal aorta and approach the origins of the neighboring intersegmental arteries to join them, and then replace the latter.
Consequently, the primary subclavians appear to be ”the lateral branches of the in tersegmental arteries.” (4) The 8th primary
subclavian is dominant at first, but is replaced by the 7th primary subclavian, which develops into the definitive subclavian
artery. (5) With the brachial nerve plexus formation, the axillary arterial plexus derived from the longitudinal trunk develops
to form two stems of the axillary artery.
Accepted: 15 April 1999 相似文献
89.
Ambient temperature related sleep changes in rats neonatally treated with capsaicin. PHYSIOL BEHAV 00(0) 000-000, 2004. The study was conducted on adult male Wistar rats, neonatally treated with capsaicin to destroy the peripheral warm receptors. The sleep-wakefulness was recorded for 5 h at an ambient temperature (T(amb)) of 18, 24, 30 and 33 degrees C on different days. The rectal temperatures (T(r)) of the rats were studied on exposure to 6 and 37 degrees C for 2 h to assess their thermoregulatory ability. The changes in the behavioral thermoregulation were assessed by noting the thermal preference of rats when they were placed in an environmental chamber with 3 interconnected compartments maintained at 24, 27 and 30 degrees C. Slow wave (SWS) and rapid eye movement (REM) sleep were decreased at 18 degrees C and increased at 30 degrees C, in control rats. There was a decrease in REM sleep and no change in SWS when T(amb) was raised from 30 to 33 degrees C. However, in neonatally capsaicin treated rats, sleep was increased even at 33 degrees C, though there was no significant change in sleep when T(amb) was increased from 18 to 24 degrees C. Capsaicin treated rats showed thermoregulatory deficiency at 37 degrees C but the thermal preference was unaltered in these rats. The results suggest that the central warm receptors can produce alteration in sleep at different T(amb), even in absence of peripheral warm receptors. The behavioral thermoregulation was unaffected in these rats, though their ability to defend the body temperature in warm environment was affected. 相似文献
90.
S. Cheng P. V. Komi H. Kyröläinen D. H. Kim K. Häkkinen 《European journal of applied physiology》1989,59(1-2):128-130
Summary Vibrational wave propagation was tested in vivo on the tibial bone of both legs of 56 female volunteers. The impact was produced
by a hammer with a force strain gauge and the response was monitored by two accelerometers. The peak amplitude of the accelerations,
the velocity of the acceleration wave propagation and damping were analysed for comparison among the different age groups.
The results showed significant negative correlations between age and the peak amplitude of acceleration, and the velocity
of acceleration wave propagation (p<0.01). The damping time of the acceleration wave also had a negative correlation with age. These findings suggested that
age differences were related to the differences in the mechanical properties of bone. With reduction of bone mineral density,
the velocity of the vibrational wave propagation would decrease, with simultaneous increase in impedance. In addition, wave
absorption would be accelerated. It is suggested that this method could be used as an indicator of bone density. The method
could also be developed to provide an index to monitor the progress of osteoporosis.
Visiting research assistant from the Department of Sport Medicine, Chengdu College of Physical Education, Chengdu, People's
Republic of China
Visiting research assistant from Seoul National University, Seoul, South Korea 相似文献