生物化学课程现状分析与改革方向

生物化学是医学重要的基础课。为加强学生素质教育，加强语文、数学等文化课学习，为高职专输送人才，在教育主管部门指导下，在课程改革方面作了大量的工作。但是经过几年的课程改革实验过程也慢慢出现了很多的问题如课程合并的不合理性、轻视医学基础课教学、课程学时分配不合理等，作者就本校的生化教学一些情况，结合中专卫校课程现状，对中专生物化学改革中出现的一些问题做粗略分析，在今后课程改革方向应向重新整合课程、强调“淡化学科”意识思想、实现医学教学模式转变、优化教学手段方法等方面发展。     

梅花鹿血液药用机理初步研究

对8只雄性梅花鹿血液血象、生化指标、微量元素和蛋白组分检测证实：梅花鹿血的RBC为1250万／mm~3,红细胞平均Hb含量为38．7Pg,红细胞平均Hb浓度970g／L,都远远高于人正常生理值。所检测的7种主要酶也远远高于人正常生理值,其中磷酸肌酸激酶（CPK）、α一羟丁酸脱氢酶（HBD）、磷酸肌酸激酶辅酶分别为327u／L、639u／I。、772u／L,是人正常生理值的7～10倍。梅花鹿血清中磷的含量是2．68mmol／L、锌为51．3μmol／L、铜为21．4μmol／L、铁为255.6μmol／L、锰0．16μmol／L,。该5种微量元素的含量都远远高于人血清正常值。在蛋白组分中白蛋白水平较低为34．9g／L,,而球蛋白水平较高为35．5g／L,其中以γ一球蛋白含量最高．为25．2％,是人血清正常值的3倍。其药用价值是明显的。     

Serum vitamin A in premature and term neonates

Serum vitamin A was determined in premature and term neonates by a specific spectrofluorometric method. Premature neonates (N = 42; gestational age = 32 +/- 0.4 weeks) had a serum vitamin A level (14.9 +/- 0.98 microgram/dl) significantly lower (P less than 0.001) than that of term neonates (N = 51; 22.4 +/- 0.99 microgram/dl). The vitamin A mean serum values of infants of 36 weeks' gestational age were not statistically different from those of the term neonates. Linear regression analysis for serum vitamin A values vs gestational age showed no significant correlation. A linear correlation (P less than 0.05), however, was found between serum vitamin A and serum protein protein concentrations, perhaps indicative of a lower concentration of retinol-binding protein. Since vitamin A is involved in the promotion of mucous-secreting cells, the premature neonate may be at greater risk than the term infant for diseases involving the mucosal epithelium, including necrotizing enterocolitis.     

Depression of blast transformation of peripheral leukocytes by plasma from pregnant women.

The blast transformation of peripheral leukocytes from pregnant and nonpregnant women has been studied at different time intervals with graded concentrations of phytohemagglutinin. The transformation is markedly depressed during pregnancy. Leukocytes from nonpregnant women and men show diminished transformation when cultured in a medium containing plasma from pregnant women.     

Characterization of kallikrein from human saliva isolated by use of affinity chromatography

Kallikrein was isolated from paraffin stimulated saliva by use of two steps affinity chromatography. A 610-fold purification of the enzyme was achieved by use of Sepharose 4B conjugated with soy bean trypsin inhibitor and pancreatic trypsin inhibitor. The isoelectric point of kallikrein was found to be pH 4.3 and the molecular weight was calculated to be about 38.000 by gel filtration on a AcA 44 Ultrogel column. The enzyme had a pH optimum at pH 8.6 using BAEE and TAME as substrates. The michaelis constant for kallikrein on these two substrates was 4.0. 10^?4M and 5.5. 10^?4M. respectively.     

早发冠心病患者脂蛋白a与临床类型及冠状动脉病变关系的初步研究

目的　探讨早发冠心病患者血浆脂蛋白a[Lp(a) ]水平与冠状动脉病变程度及临床类型的关系。方法　采用Judkins法对 12 6例胸痛或胸部不适患者行冠状动脉造影 ,确诊为早发冠心病患者 ,以Lp(a)水平差异分为两个亚组 ,并与 89例对照者进行比较分析。结果　早发冠心病组血浆Lp(a)为 (2 33± 114 )mg/L明显高于对照组 (190± 78)mg/L(P <0 .0 5 ) ;早发冠心病患者Lp(a)低于 30 0mg/L亚组中 ,心肌梗死、不稳定型心绞痛和稳定型心绞痛比例无明显差异 ;而在Lp(a)异常升高亚组 ,临床表现为不稳定型心绞痛的比例最高 (5 4 % ) ,明显高于另两种临床类型 (P <0 .0 5 )。冠状动脉病变高积分组Lp(a)水平明显高于中等积分组和低积分组 (P <0 .0 1或 0 .0 5 )。结论　早发冠心病患者血浆Lp(a)水平与临床类型及冠状动脉病变程度密切相关     

Enzymic determination of urinary free l-fucose

Conditions necessary for the precise measurement of free l-fucose in urine by an enzymic method using l-fucose dehydrogenase have been studied. The normal urinary levels of l-fucose were 16.4 ± 9.1 μg/ml in children (22.2 ±6.5 μg/mg of creatinine) and 17.7 ± 8.5 μg/ml in adults (16.6 ± 5.7 μg/mg of creatinine). There was a close correlation between the concentration of free l-fucose and that of creatinine. A close correlation was also found between the concentration of free l-fucose and α-l-fucosidase activity. Thus, it was suggested that the free l-fucose in urine reflected the metabolism of l-fucose or l-fucose-containing glycoconjugates.In a preliminary screening test, several urine samples which showed high concentrations of free l-fucose, namely cases of “fucosuria” were found.     

A sandwich enzyme immunoassay of human growth hormone in serum using anti-human growth hormone IgG-beta-D-galactosidase conjugate

The specificity, sensitivity and stability of beta 2-microglobulin (beta 2-m) and retinol-binding protein (RBP) in urine as indices of tubular proteinuria were compared. In vitro experiments show that RBP in urine is stable down to pH 4.5, whereas beta 2-m degrades below pH 5.5. This was confirmed by the relationships between pH and the concentration of both proteins in the urines from 150 healthy subjects. Urinary RBP was independent of pH (r = 0.125) but in contrast at pH below 6, beta 2-m concentration was inversely correlated with pH (r = 0.54, p less than 0.05). The comparison of urinary excretion of RBP and beta 2-m in 68 patients with renal diseases shows that in absence of a pH effect, the sensitivity and specificity of both proteins as indices of tubular proteinuria are rather similar. Therefore, in view of its greater stability in urine, RBP appears to be a more practical and reliable index of proximal tubular function than beta 2-m.