溶血、温度以及储存时间对肝功能生化检测的影响

目的评价标本溶血、温度以及储存时间对肝功能生化检测效果的影响。方法选取2011年3月～2013年7月来我中心进行健康体检的正常人群90例,随机平均分为A、B、C三组,研究溶血、温度以及储存时间对ALT（谷丙转氨酶）、AST（谷草转氨酶）、TBiL（总胆红素）和DBiL（直接胆红素）的影响。结果A组中比较发现溶血组与不溶血组AST、TBiL和DBiL差异有统计学意义（P〈0．05）,ALT比较差异无统计学意义（P〉0．05）;B组与C组中比较差异均无统计学意义（P〉0．05）。结论在肝功能生化检测中,溶血能影响AST、TBiL和DBiL水平,温度以及储存时间对检验结果无影响,所以在检验过程中应注意防止样本溶血。     

血清生化指标与冠心病患者冠状动脉狭窄程度的相关性

目的探讨血清生化指标与冠心病患者冠状动脉狭窄程度的相关性。方法选取上海市、武汉市和福州市3家三级甲等综合性医院临床诊断为冠心病且接受冠状动脉造影检查的患者574例,记录患者在经皮冠状动脉介入治疗(PCI)术中得到的冠状动脉狭窄程度数据,量化后计算Gensini积分,同时收集患者临床资料并检测血清生化指标,项目包括总胆红素(TB)、直接胆红素(DBil)、总蛋白(TP)、白蛋白(Alb)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、γ-谷氨酰基转移酶(GGT)、乳酸脱氢酶(LDH)、空腹血糖(FPG)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂蛋白(a)[Lp(a)]、载脂蛋白A1(apo A1)、载脂蛋白B(apo B)、高敏C反应蛋白(hs-CRP)。采用Logistic回归分析评估各项指标与冠状动脉狭窄程度的相关性。结果高Gensini积分组男性所占比例及TP、Alb、ALT、AST、FPG、HDL-C、Lp(a)、apo A1、apo B、hs-CRP水平与低Gensini积分组比较,差异均有统计学意义(P<0.05)。其他项目2个组之间差异均无统计学意义(P>0.05)。Logistic回归分析结果显示,Alb降低、AST升高、FPG升高、HDL-C降低、Lp(a)升高及男性为冠状动脉狭窄程度增加的危险因素[比值比(OR)值分别为0.932、1.011、1.121、0.299、1.015、1.753,95%可信区间(CI)分别为0.886~0.98、1.002~1.02、1.024~1.227、0.143~0.624、1.002~1.008、1.110~2.767]。结论FPG和Lp(a)是预测冠心病患者冠状动脉狭窄程度的重要风险因素,可用于冠心病患者冠状动脉病变情况的初步判断。AST、Alb和HDL-C能否作为冠状动脉狭窄程度的预测指标仍需进一步研究。     

Corrective Effect of Verbascoside on Histomorphological Differences and Oxidative Stress in Colon Mucosa of Rats in Which Colon Ischemia–Reperfusion Injury was Induced

BackgroundThis study aims to show the corrective effect of verbascoside on histomorphological and biochemical differences in the colon mucosa of rats in which colon ischemia–reperfusion (I/R) injury was induced.Methods: Fifty Sprague Dawley male rats were divided into 5 groups, of control, sham, ischemia (I), I/R, and I/R+verbascoside.Ischemia and reperfusion were applied to the suitable groups for 30 minutes and 120 minutes respectively, and 10 mg/kg verbascoside was administered intraperitoneally. Histomorphological assessment was done in the colon tissues obtained, and the goblet cells were assessed using the Alcian blue method. Proliferating cell nuclear antigen (PCNA), TUNEL, and hypoxia-induced factor 1 (HIF-1α) assays were used to assess oxidative stress with the immunohistochemical method. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), and total thiol (TT) levels were checked, for a biochemical analysis of oxidative stress.Results: Compared with the I/R group, histomorphological differences were seen to be corrected in colon epithelium in the I/R+verbascoside group. The goblet cell number increased and cell proliferation was increased, as seen with the PCNA assay; and apoptosis was decreased, as seen with the TUNEL assay. HIF-1α expression also decreased in the drug group. In the drug group, SOD, GSH-Px, TAS, and TT levels increased, but TOS, OSI, and MDA levels decreased.Conclusion: It was seen that verbascoside had a corrective effect on histomorphological and biochemical differences caused by I/R injury.     

铁碳微电解--生化法处理医药废水

针对浙江某医药原料厂高浓度有机废水提出了一种新型的铁碳微电解-生化处理方法,介绍了其预处理原理、工艺流程及主要技术工艺参数.工程运行结果表明,经处理后废水的B/C值明显提高,酸度显著下降,达到了污水二级排放标准.     

Evaluation of a data-base

The records of 421 psychiatric patients were analysed by a consistent method, allowing an optimal use of indicant-patterns. It was assumed that such a procedure would give a satisfactory predictive capacity, even for operational criteria as for example choice of treatment, duration of stay in hospital, or a global evaluation of outcome. The study demonstrates, however, that fundamental changes in the data-base are necessary. Problems of reliability, validity, sensitivity and specificity are discussed and some suggestions are offered for the future improvement of psychiatric data-bases.     

产后抑郁症发病机制中生物化学因素的研究进展

产后抑郁症是产褥期发生的抑郁症,是指以往无精神疾病史的患者出现以持续性情感低落为主的精神健康疾患,在发病过程中常常伴随着思维以及行为的改变,是最为常见的一种产褥期精神综合征.产后抑郁不仅对产妇的生活造成巨大影响,对婴儿的正常发育成长也有影响,严重抑制婴儿正常认知的发展.研究产后抑郁症的发病因素对于疾病预防及治疗具有十分重要的意义,该文综合了近几年来研究人员对产后抑郁症发病机制中生物化学因素的研究成果,从激素、神经递质、及其他因子等方面加以综述,为临床治疗提供依据.     

Clinical value of cholinesterase in the prediction of biochemical recurrence after radical prostatectomy

Purpose

To evaluate the predictive and prognostic role as well as the clinical impact on decision-making of serum cholinesterase (ChoE) levels in patients treated with radical prostatectomy for clinically nonmetastatic prostate cancer.

骨转移生化指标在乳腺癌诊治中的应用

目的：介绍骨生化指标在乳腺癌骨转移诊治中的应用及价值。方法：分析总结近8年来国、内外关于骨生化指标在乳腺癌骨转移诊治中的应用研究。结果：破骨型生化指标氮端肽(NTX)与骨转移的相关性最好，Ⅰ型胶原碳端肽(ICTP)在乳腺癌骨转移诊治中比NTX更有价值；成骨型生化指标在乳腺癌骨转移诊治中的价值不大。结论：骨生化指标尚不能取代影像学及骨活检在乳腺癌骨转移诊治中的地位。     

慢性病毒性肝炎患者血清铁检测与肝脏生化及病理的关系

目的探讨血清铁与慢性肝炎血生化指标及病理炎症分级(G)、纤维化分期(S)及分度(S)的相关性及血清铁的检测意义。方法对455例慢性肝炎患者血清铁检测,并与肝脏生化指标、肝炎炎症分级、纤维化分期和分度做直线相关分析,同时分析轻、中、重度慢性肝炎血清铁是否存在差异。结果血清铁与ALT、AST、BIL呈正相关;而与CHE呈负相关;与肝脏病理指标中的G、S、D均呈正相关。血清铁均值随慢性肝炎程度的加重而增高。结论血清铁能一定程度上反映肝脏病变程度,但特异性较差。