宫内胎肝细胞移植形成血小板GPⅡb Bak a/b嵌合体的研究

目的　探讨宫内胎肝细胞移植是否形成人工血小板杂合子嵌合体 ,评估其在治疗血小板无力症的临床应用前景。方法　选择血小板膜GPⅡbBaka/b(GPⅡb异亮氨酸 84 3丝氨酸多态性 )作为遗传标记 ,采用等位基因特异引物PCR和FOKⅠ限制性内切酶消化技术从 4 2名引产妇筛选一例纯合子GPⅡbBaka/a作为供者 ,一例纯合子GPⅡbBakb/b作为受者 ,制备供者胎肝细胞 ,取 4ml(2 2× 10 5细胞 )在B超引导下通过脐静脉穿刺输给受者。结果　移植后 2 1天 ,用PCR FOKⅠ消化技术检测引产后受者外周血DNA和血小板RNA ,发现已形成Baka/b嵌合体。结论　我们认为宫内胎肝细胞移植可能提供一种治疗血小板无力症和其他遗传性疾病的方法。     

糖尿病患者胰岛素基因启动子的突变检测和临床应用

目的 :探讨胰岛素基因启动子突变是否与中国人 2型糖尿病相关。方法 :随机选择黑龙江省中国汉族人 89例 2型糖尿病 ,应用聚合酶链式反应———单链象多态性 (PCR -SS CP)检测方法检测胰岛素启动子突变。结果 :89例 2型糖尿病中未发现 1例异常泳动变化。结论 :胰岛素基因启动子突变可能不是中国人 2型糖尿病的重要遗传因素。文献报道美国黑人中胰岛素基因启动子突变 ( 8个碱基TGGTCTAA的重复序列 )与 2型糖尿病相关 ,本研究结果提示此种相关有明显的种族异质性     

胶体金层析法检测恶性疟原虫HRPII抗原的应用研究

目的 :建立一种简易快速 ,适于基层或流行病学调查的自测式胶体金免疫层析法 (GICA)用于检测恶性疟原虫中 H RPII抗原 ,判定是否现症感染。方法 :用 GICA测试条检测全血及滤纸血样中的 H RPII抗原。结果 :40例恶性疟全血及滤纸血 H RPII抗原阳性检出率均为 1 0 0 ,与镜检结果相符合 ;2 5例间日疟均为阴性。结论 :GICA检测血中恶性疟原虫 H RPII抗原特异性强、灵敏度高、简便快速 ,无需特殊仪器设备 ,滤纸血的应用使样本的采集、保存和运输更为便利 ,适合大规模流行病学调查和基层使用 ,有广泛应用价值。     

应用动态血压观察氯沙坦钾治疗难治性高血压的临床疗效

目的 :观察氯沙坦钾治疗高血压的临床疗效。方法 :选择 3 2例其他降压药物疗效不佳的高血压患者 ,年龄 4 1～ 67( 55.6± 6.5)岁 ,服用氯沙坦钾 50 mg～ 1 0 0 mg/d,总疗程为 6w。全部病人在服用氯沙坦钾前和服用氯沙坦钾 6w后均行动态血压监测 ( ABPM)、偶测血压及肝肾功能、血脂和血糖检查。结果 :1 .用药后 2 4 h平均血压、日昼和夜间平均血压比用药前下降 ( P<0 .0 1 ) ,用药后收缩压及舒张压负荷均较用药前明显下降 ( P<0 .0 1 )。 2 .偶测血压疗效　服氯沙坦钾降压有效率为 1 0 0 % ,其中 2例需 1 0 0 mg/d,1例加用了双氢克尿噻 1 2 .5mg/d。 3 .降压作用的谷 /峰比值　氯沙坦钾降低收缩压和舒张压的谷 /峰比值分别为 71 .6%和 68.1 %。结论 :Ang II受体拮抗剂是继 ACEI之后的新一类抗高血压药物 ,与 ACEI相比 ,此类药物直接作用于AT1受体 ,降压作用安全可靠 ,副作用少 ,对于其他降压药物疗效不佳的高压患者也有良好效果     

自发性高血压大鼠左心室肥厚与血压、心肌局部血管紧张素Ⅱ的关系

目的 研究自发性高血压大鼠(SHR)左心室肥厚与血压、心肌局部血管紧张素Ⅱ的关系。方法 取SHR(n=40)及正常对照组WKY(n=41),分为4周龄、8周龄、12周龄、24周龄及36周龄组．测收缩压、体重,左心室重量及用放免法测定心肌局部血管紧张素(AⅡ)含量,并进行两两线性相关分析。结果 通过对5个年龄段的SHR和WKY的收缩压、左心室重与体重比(LVM／BW)及心肌局部AⅡ含量的测定结果发现三者之间存在相关性,其中AⅡ与心肌肥厚有明显相关性。结论 血压与AⅡ在SHR大鼠的左心室肥厚中起着重要作用。     

Angiotensin converting enzyme inhibitor-induced angioedema: a report of two cases

Angioedema is a rare but potentially fatal side effect of angiotensin converting enzyme (ACE) inhibitors. We report for the first time, two children with systemic lupus erythematosus who developed acute angioedema after the long-term use of enalapril. Prompt recognition and appropriate management of ACE-induced angioedema prevented life-threatening complications. This report highlights the potential risks of angioedema associated with the use of ACE inhibitors in children. Patients should be advised to seek medical treatment immediately if they experience swelling of the face, neck, or tongue, and especially if they have trouble breathing, speaking, or swallowing. Received: 12 March 1999 / Revised: 8 June 1999 / Accepted: 8 June 1999     

洛沙坦抗高血压及左室肥厚的疗效观察

目的 :观察血管紧张素 受体拮抗剂—洛沙坦的降压效果及对高血压病合并左室肥厚的影响。方法 :4 6例合并左室肥厚的 期高血压病患者服用洛沙坦 5 0 mg/ d,观察其血压的变化及治疗前和 6个月后左室质量 (L VM)。结果 :用洛沙坦后 4 d～ 6d血压开始下降 ,2周血压趋向正常 ,4周血压继续缓慢下降 ,6周时达到最大降压效果。 L VM在 12周时无明显变化。 2 4周表现轻度 L VM减少。结论 :洛沙坦是抗高血压的一个有效治疗药物 ,对左心室肥厚有轻度逆转作用     

Spike conduction properties of T-shaped C neurons in the rabbit nodose ganglion

Noradrenaline (NA) and angiotensin II (A II) were infused intravenously in conscious dogs without (series I) and with (series II) additional infusions of sodium nitroprusside at doses re-establishing normal levels of mean arterial pressure (MAP). In series I, NA infusion (1.6 g/min per kg for 30 min) initially elevated MAP by some 25 mm Hg and lowered heart rate by some 30 beats/min. Plasma concentrations of arginine vasopressin (AVP) remained constant, while those of A II and atrial natriuretic factor were slightly, but significantly, increased. Infusion of A II (10 or 20 ng/min per kg for 30 min) induced similar rises in MAP and slight reductions of heart rate and increased plasma AVP by 70% and atrial natriuretic factor by 60%. In series II, sodium nitroprusside (1–4 g/min per kg) was added for 30 min to infusions of NE (1.6 g/min per kg) and A II (20 ng/min per kg) in order to maintain MAP at its control level. This resulted in an 11-fold increase in plasma AVP during NA infusion and a 19-fold increase during A II infusion. Infusing sodium nitroprusside (4 g/min per kg) alone lowered MAP to clearly hypotensive levels, but the resulting rises in plasma AVP were less than, rather than equal to, those seen at normotensive MAP levels during the combined infusions of sodium nitroprusside with A II or NA, respectively. It is concluded that both NA and A II exert strong stimulatory actions on AVP release which are, however, counteracted by inhibitory influences arising from the hypertensive effects of NA and A II.     

The effect of angiotensin II on haemodynamic and plasma noradrenaline responses to tyramine infusion in man

Summary Six normal volunteers were studied on four separate occasions. On each occasion they received two concomitant infusions which were either placebo/placebo, placebo/tyramine, angiotensin II/placebo or angiotensin II/tyramine. Angiotensin II infusion was given at a constant rate of 2ng/kg/min whereas the tyramine infusion consisted of 10 min increments at 1.25, 2.5, 3.75, 5, 7.5 and 10 g·kg^–1·min^–1.Tyramine infusion caused a dose dependent increase in systolic blood pressure with increases in diastolic blood pressure and plasma noradrenaline only at the highest doses. These changes were not affected by concomitant angiotensin infusion.We have therefore found no evidence to support the enhancement of haemodynamic or plasma noradrenaline responses to tyramine infusion by low dose infusion of angiotensin II in man.     

肾虚型糖尿病肾病与Ⅰ型血管紧张素Ⅱ受体基因多态性相关性研究

目的:探讨Ⅰ型血管紧张素Ⅱ受体(AGT1R)基因多态性与肾虚型糖尿病肾病的关系。方法:将240例2型糖尿病分为糠尿病肾病(DN)组和非DN组,进一步分为肾阳虚证、肾阴虚证及非肾虚证,用PCR-RFLP方法检测AGT1R基因型。结果:2组肾阳虚、肾阴虚型AGT1R-A1166C的AC型频率和C等位基因的携带率明显高于非肾虚型,非DN组非肾虚型AC型占3.4%,而DN组肾阳虚型为8.4%、肾阴虚型为10.8%(均P<0.05);非DN组非肾虚型C等位基因占1.7%,而DN组肾阳虚型为4.2%、肾阴虚型为5.4%(均P<0.05);但肾阳虚型与肾阴虚型间差异无显著性意义(P>0.05)。结论:A1166-C多态与肾虚型DN的发生显著相关,提示AGT1R基因的不同基因型与等位基因可能是DN患者肾虚证的物质基础。