Comparative Analysis of Postoperative Complications of Sentinel Node Identification Using the SentiMag® Method and the Use of a Radiotracer in Patients with Breast Cancer

(1) Background: The purpose of the study was a retrospective, comparative assessment of complications of the surgical sentinel node biopsy (SNB) procedure in breast cancer using the radiotracer method and the SentiMag^® method on groups of patients after 3.5 years of use. (2) Methods: The material was a group of 345 patients with primary surgical breast cancer who underwent the SNB procedure with the use of a radiotracer in combination with wide local excision (WLE), simple amputation (SA) with SNB and an independent SNB procedure in the period from May 2018 to January 2021 in the Department of Oncological Surgery. Of the patients who were monitored in the Hospital Outpatient Clinic, 300 were enrolled. The analyzed group was compared in terms of the occurrence of the same complications with the group of 303 patients also operated on in our center in the period from January 2014 to September 2017, in which SN identification was performed using the SentiMag^® method. (3) Results: The most common complications found were sensation disorders in the arm, which occurred in 16 (14.1%) patients using the radiotracer method, SentiMag^®-11 (9.9%). By comparing the complication rate between the methods with the radiotracer (n = 300) and SentiMag^® (n = 303), no significant differences were found. (4) Conclusions: Sentinel node (SN) identification using the radiotracer method and the SentiMag^® method are comparable diagnostic methods in breast cancer, with a low risk of complications.     

Fibrin glue assisted amniotic membrane graft reconstruction of conjunctival fornix and canthus following excision of conjunctival tumours

Conjunctival tumors involving non-limbal locations, such as the fornix and canthus, are typically excised using a “non-touch” technique, often with a wide surgical margin. Reconstruction of these large defects can be difficult due to the contour of the ocular surface and are often complicated by shortening of the fornix, symblepharon formation, and restriction of eye movements. In our experience, the use of amniotic membrane grafts combined with the sealant properties of fibrin glue such as Tisseel® has improved our surgical outcomes during the reconstruction phase. We would like to highlight and describe our surgical technique using fibrin glue and squint hooks to aid amniotic membrane graft reconstruction for surgically challenging locations in the fornix and canthus following excision of conjunctival lesions, with excellent surgical outcomes.     

培育颗粒对人绒毛滋养细胞侵袭与增殖的作用

目的观察培育颗粒对人绒毛滋养细胞的侵袭和增殖功能的影响。方法取人妊娠5~10周正常胎盘绒毛,进行滋养细胞培养。正常23~25 d雌性SD大鼠药物灌胃,取药理血清。培养液加20%血清,分组培养48 h后检测指标。用Transwell侵袭实验检测培育颗粒对滋养细胞侵袭功能的影响,MTT法检测培育颗粒对滋养细胞增殖能力的影响,流式细胞术检测培育颗粒对滋养细胞增殖核抗原(PCNA)表达的影响。结果Transwell侵袭实验检测结果表明,培育颗粒血清各剂量组与正常组侵袭细胞数均有差异,中剂量组侵袭细胞数最多。MTT法检测结果表明,培育颗粒血清各剂量组与正常组吸光度均有差异,中剂量组吸光度最高。流式细胞术检测结果表明,培育颗粒血清各剂量组与正常组PCNA阳性率均有差异,中剂量组PCNA阳性率最高。结论培育颗粒可促进人绒毛滋养细胞侵袭与增殖功能,可能与负反馈调节有关。     

羊膜移植联合结膜瓣遮盖术防止角膜穿孔

目的 探讨羊膜移植联合结膜瓣遮盖术防止角膜穿孔的临床疗效.方法 采用羊膜移植联合结膜瓣遮盖治疗难治性角膜炎致角膜濒临穿孔24例,术后继续局部药物治疗,随访3-10个月,观察角膜愈合情况.结果 术后均未见羊膜植片的急性排斥反应,所有手术病例无一出现角膜穿孔,角膜溃疡逐渐修复,角膜基质有不间程度的增厚,多数患者术后视力均有不同程度的提高.结论 羊膜移植联合结膜瓣遮盖是治疗角膜濒临穿孔的有效方法.     

姜黄素抑制磨损颗粒诱导炎性介质产生及作用机制

目的观察姜黄素对磨损颗粒刺激炎性介质产生的影响,进一步探讨炎性介质表达调节机制。方法构建小鼠air-pouch动物模型成功后,于囊腔中注射高分子聚乙烯颗粒悬液。随机分为实验组和对照组,分别每日每次0.6ml,浓度为3.2g.L-1姜黄素溶液灌胃和等量6%的聚乙二醇溶液灌胃,共2wk,给药后2wk处死取材,进行组织学观察,采用ELISA法及半定量RT-PCR检测假体周围组织中TNF-浕、IL-1β、EMMPRIN、MMP-9的表达。Western blot检测囊壁组织核蛋白中NF-κBP65蛋白的表达。结果组织学观察发现对照组的囊腔范围及囊壁厚度明显大于给药组。HE染色囊壁中有大量炎性细胞,两组间炎性细胞计数差异有统计学意义;两组囊壁组织中TNF-浕、IL-1β、EMMPRIN、MMP-9、NF-κBP65表达有统计学意义。结论姜黄素可以抑制air-pouch动物模型界膜组织中TNF-浕、IL-1β、EMMPRIN、MMP-9的表达,可能是通过下调囊壁组织中核蛋白NF-κB完成的。     

深低温保存羊膜在眼睑成形术中的应用

观察和评价羊膜在眼睑缺损成形术中的应用价值。方法采用保存人羊膜移植用于各种原因引起的眼睑缺损部位替自体球结膜移植,成形再造眼睑共２６例２６只眼。结果２６只眼无１例感染,１０天后移植的羊膜变透明,缝合处结膜向羊膜上爬行,１个月后新生的结膜上皮完全覆盖移植区。     

银桔颗粒抗炎作用的实验研究

目的研究银桔颗粒的抗炎作用及作用机制。方法对鸡蛋清致大鼠足跖炎性组织中超氧化物歧化酶（SOD）活力测定,进行体外抑菌实验考察银桔颗粒的抗炎抑菌作用,并观察小鼠腹腔内巨噬细胞对红细胞的吞噬功能。结果与对照组比较,大、中、小剂量组大鼠足跖炎性组织中SOD含量差异均有统计学意义（均P〈0．01）,小鼠腹腔巨噬细胞对红细胞的吞噬百分率及吞噬指数均显著增高（均P〈0．01）。银桔颗粒粒对4种致病菌的最低抑菌和最小杀菌浓度（MBC）分别为：肺炎链球菌（MIC0．286g/ml,MBC0．429g／ml）;克雷伯菌（MIC0．140g／ml,MBC0．275g／ml）;铜绿假单胞菌（MIC0．0177g／ml）,MBC0．0358g／ml）;大肠杆菌（MIC0．429g／ml,MBC0．429g／ml）。结论银桔颗粒具有良好的抗炎抑菌增加免疫力作用。     

子宫动脉栓塞术治疗子宫肌瘤21例临床分析

目的 探讨子宫动脉栓塞术(UAE)治疗子宫肌瘤的疗效。方法 选择21例子宫肌瘤病人，采用Seldinger技术行双侧子宫动脉插管，造影确认后注入PVA栓塞颗粒。结果 UAE治疗3个月后，子宫肌瘤病人的临床症状明显缓解，月经恢复正常，肌瘤体积缩小(P＜0．01)。保留了子宫完整的生理功能。结论 UAE治疗子宫肌瘤有其特殊的优越性，为子宫肌瘤病人提供了另一种可选择的治疗方法。     

益肾助孕颗粒对围绝经期大鼠卵巢VEGF表达的影响

目的:观察中药益肾助孕颗粒对围绝经期大鼠卵巢血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响.方法:通过自然老化法构建围绝经期大鼠模型,选择处于围绝经期的大鼠(12 ～ 14月龄)40只,随机分为老年大鼠对照组、益肾助孕颗粒高、中、低剂量组.每组10只,分别用生理盐水2 mL灌胃、益肾助孕颗粒按等效剂量高剂量10 g·kg-1 ·d-1,中剂量5 g·kg-1 ·d-1,低剂量为2.5 g·kg-1 ·d-1,灌胃0.2 mL·g-1·d-1灌胃4周;另取5月龄大鼠10只作为青年大鼠对照组,生理盐水2 mL灌胃4周.免疫印迹法定量检测各组大鼠卵巢VEGF蛋白质的表达;RT-PCR检测各组大鼠卵巢VEGF mRNA的表达.结果:治疗前,与青年大鼠组VEGF蛋白、VEGF mRNA比较,老年对照组和研究组大鼠卵巢的VEGF蛋白、VEGF mRNA都表达明显高于青年组,差异显著,有统计学意义(P＜0.05),用药4周后,与老年对照组大鼠卵巢VEGF蛋白、VEGF mRNA表达比,研究组大鼠卵巢VEGF蛋白、VEGF mRNA表达明显下降,差异显著,有统计学意义(P＜0.05),并存在剂量依赖性.结论:益肾助孕颗粒能够下调围绝经期大鼠卵巢VEGF蛋白、VEGF mRNA的表达,可能通过调节VEGF治疗围绝经期综合征.     

Low-density lipoprotein encapsulated thiosemicarbazone metal complexes is active targeting vehicle for breast,lung, and prostate cancers

Cancer is a leading cause of death worldwide and affects society in terms of the number of lives lost. Current cancer treatments are based on conventional chemotherapy which is nonspecific in targeting cancer. Therefore, intensive efforts are underway to better target cancer-specific cells while minimizing the side effects on healthy tissues by using LDL particles as active drug delivery vehicles. The goal is to encapsulate anticancer agents thiosemicarbazone metal-ligand complexes into LDL particles to increase the cytotoxic effect of the agent by internalization through LDL receptors into MCF7, A549, and C42 cancer cell lines as segregate models for biological evaluations targeting tubulin. Zeta potential data of LDL-particles encapsulated anticancer agents showed an acceptable diameter range between 66–91 nm and uniform particle morphology. The results showed cell proliferation reduction in all tested cell lines. The IC₅₀ values of LDL encapsulated thiosemicarbazone metal-ligand complexes treated with MCF7, A549, and C42 ranged between 1.18–6.61 µM, 1.17–9.66 µM, and 1.01–6.62 µM, respectively. Western blot analysis showed a potent decrease in tubulin expression when the cell lines were treated with LDL particles encapsulated with thiosemicarbazone metal-ligand complexes as anticancer agents. In conclusion, the data provide strong evidence that LDL particles are used as an active drug delivery strategy for cancer therapy.