Antigen presentation by proliferating thymic macrophages to A (T,G)-A-L specific T cell line in an H-2 restricted manner

Long-term cultures of murine homogeneous populations of Ia-bearing thymus-derived murine macrophages were tested for their ability to present antigen to a (T,G)-A-L specific IL-2-dependent continuous T cell line. Thymus-derived macrophages, with and without pretreatment for Ia induction, triggered efficiently antigen-specific T cell proliferation in an MHC restricted way. This experimental system, consisting of two normal proliferating homogeneous populations of macrophages and antigen specific T cells, provides an ideal tool for studying the mechanism of antigen presentation to T cells and for elucidation of the role of macrophages in T-B cell collaboration for antibody production.     

Clinical safety and effectiveness evaluation of a new antimicrobial wound dressing designed to manage exudate,infection and biofilm

The objective of this work was to evaluate the safety and effectiveness of a next‐generation antimicrobial wound dressing (NGAD; AQUACEL ^® Ag+ Extra™ dressing) designed to manage exudate, infection and biofilm. Clinicians were requested to evaluate the NGAD within their standard protocol of care for up to 4 weeks, or as long as deemed clinically appropriate, in challenging wounds that were considered to be impeded by suspected biofilm or infection. Baseline information and post‐evaluation dressing safety and effectiveness data were recorded using standardised evaluation forms. This data included wound exudate levels, wound bed appearance including suspected biofilm, wound progression, skin health and dressing usage. A total of 112 wounds from 111 patients were included in the evaluations, with a median duration of 12 months, and biofilm was suspected in over half of all wounds (54%). After the introduction of the NGAD, exudate levels had shifted from predominantly high or moderate to low or moderate levels, while biofilm suspicion fell from 54% to 27% of wounds. Wound bed coverage by tissue type was generally shifted from sloughy or suspected biofilm towards predominantly granulation tissue after the inclusion of the NGAD. Stagnant (65%) and deteriorating wounds (27%) were shifted to improved (65%) or healed wounds (13%), while skin health was also reported to have improved in 63% of wounds. High levels of clinician satisfaction with the dressing effectiveness and change frequency were accompanied by a low number of dressing‐related adverse events (n = 3; 2·7%) and other negative observations or comments. This clinical user evaluation supports the growing body of evidence that the anti‐biofilm technology in the NGAD results in a safe and effective dressing for the management of a variety of challenging wound types.     

海蒿子含氮有机化学成分研究

目的对海蒿子Sargassum pallidum全草进行含氮有机化学成分研究。方法采用D101大孔吸附树脂柱色谱、正相硅胶柱色谱、凝胶柱色谱、ODS柱色谱、高效液相色谱等方法对含氮有机化学成分进行分离纯化,并根据其理化性质和核磁共振谱、质谱等光谱学技术鉴定所分离化合物的结构。结果从海蒿子全草70%乙醇提取部位分离得到了10个化合物,分别鉴定为(-)-伞形香青酰胺(1)、(-)-栝楼酯碱(2)、(-)-金色酰胺醇酯(3)、核黄素(4)、β-腺苷(5)、2′-Ο-甲氧基尿嘧啶核苷(6)、环(L-丙氨酸-L-脯氨酸)(7)、胸腺嘧啶核苷(8)、1-(β-D-ribofuranosyl)-1H-1,2,4-triazone(9)、2,3-二氢-4(1H)-喹诺酮(10)。结论化合物1、2、4~7、9为首次从马尾藻属植物中分离得到。     

Electrochemical evidence of the adsorption of alkanethiols on two sites on Ag(111)

We present spectroscopic and electrochemical results consistent with the adsorption of alkanethiols on two sites and the formation of a very densely packed, stable, monolayer on Ag(111). A surface coverage of alkanethiols of 8.5 (±0.4)×10^?10 mol cm^?2 was calculated from the charge associated with the reduction of a monolayer of alkanethiols. Alkanethiol modified Ag(111) electrodes were found to have lower capacitances than the alkanethiol modified Au(111) electrodes. The lower capacitances are due to the higher surface concentration of alkanethiols on Ag(111). The very low intensity of the methylene CH stretching bands of alkanethiols with alkane chains of four to nine carbons chemisorbed on Ag(111) suggests a vertical orientation of all-trans alkane chains. No vibrational evidence of oxidation of the chemisorbed alkanethiols was found after exposure to air for up to 6 weeks. A fine structure consisting of two current peaks was observed in all cyclic voltammograms of the reduction of chemisorbed alkanethiols. This revealed that the reduction of chemisorbed alkanethiols occurs in two steps on Ag(111). Chronoamperometric measurements also support a two-step reductive desorption of alkanethiols. The high surface concentration of alkanethiols and the orientation of the alkane chains suggest that the fine structure in the voltammograms is due to the reduction of the alkanethiols from two adsorption sites.     

MEBO与SD-Ag在烧伤临床中的疗效观察

本文介绍我院应用MEBO及2％磺胺嘧啶银混悬液（以下简写为SD－Ag）治疗烧伤病人400例,通过临床观察表明,MEBO较SD－Ag具有明显止痛作用,抗感染力强,阻止创面加深,促进创面愈合,减少疤痕形成等特点,且使用简单,贮存、运输方便,不受环境、条件的限制,具有无可比拟的优越性和实用价值。这一新的治疗技术革新了旧的观念,充分发挥了祖国医学的优越性,给广大烧、烫伤患者带来了福音,大大减轻了他们精神和肉体上的痛苦。     

肾移植患者T淋巴细胞核仁组织区嗜银蛋白的监测

目的　研究外周血T淋巴细胞核中的rDNA转录活性在异体肾移植手术前后的变化及其敏感性。方法　异体肾移植手术前后取外周血T淋巴细胞 ,检测核仁银染面积与核面积的比值(Ag NORs) ,同时取血做T淋巴细胞表面抗原测定和病毒抗体检测。结果　外周血T淋巴细胞Ag NORs含量由术前的 (6 2 9± 0 97) % ,分别下降为术后 1周的 (4 6 3± 0 90 ) % ,术后 2周的 (4 81±1 6 ) %和术后 3周的 (4 5 6± 1 2 ) %。手术前的Ag NORs值与术后不同时期的Ag NORs值比较差异有显著性 (P <0 0 5 )。术后不同时期的Ag NORs值之间差异无显著性 (P >0 0 5 ) ;Ag NORs值不受性别及有无病毒感染的影响。T淋巴细胞表面抗原测定 ,CD3、CD4、CD8、CD4/CD8等手术前后无显著性变化 ,也不受病毒感染的影响。结论　外周血T淋巴细胞Ag NORs值可用于肾移植患者的免疫监测 ;在免疫低下人群中用Ag NORs值监测免疫状态较T淋巴细胞表面抗原测定敏感。     

结核杆菌Ag85B与ESAT-6双顺反子真核表达质粒的构建及体外表达

目的构建结核杆菌Ag85B与ESAT-6双顺反子真核表达质粒。方法采用PCR的方法从结核杆菌H37Rv基因组DNA中扩增出Ag85B及ESAT-6基因,将其分别定向克隆入真核双表达载体pIRES,构建同时表达两个目的基因的双顺反子重组质粒。进行酶切分析及序列测定后,用脂质体包裹体外转染A549细胞,RT-PCR检测Ag85B及ESAT-6的表达。结果核酸序列测定证实重组质粒构建正确;该重组质粒能在体外表达Ag85B及ESAT-6mRNA。结论成功构建了结核杆菌Ag85B及ESAT-6双顺反子真核表达质粒,并在体外实现了共表达,为进一步在整体动物水平的实验研究奠定了基础。     

Breakpoint within the nucleolus organizer region resulting in a reciprocal translocation t(4;14)(q21;p12)

Reciprocal translocations involving a break in the nucleolus organizer region (NOR) are rare. A balanced translocation in a mother and her fetus with breakpoints in the NOR at 14p12 and on the long arm of a chromosome 4 at band 4q21 is described. The rearrangement was characterized by Ag‐NOR staining, multiplex fluorescence in situ hybridization (M‐FISH), and FISH with rDNA probes. This and other cases with breakpoints within NORs are discussed. Am. J. Med. Genet. 92:264–268, 2000. © 2000 Wiley‐Liss, Inc.     

分支杆菌Ag85B蛋白对小鼠膀胱癌抑癌效应的研究

目的:探讨分支杆菌Ag85B蛋白对小鼠膀胱癌的抑癌效应。方法:采用T739 可移植性膀胱癌模型,于移植瘤处局部注射分支杆菌Ag85B蛋白,并用卡介苗(BCG)和生理盐水(NS)作对照,观察治疗后各组的肿瘤重量和存活期。结果:分支杆菌Ag85B蛋白治疗组小鼠肿瘤瘤重(4.12±0.85) g,显著低于NS组(7.01±1.18) g(P<0.01),与BCG组(3.85±1.18) g比较差异无统计学意义(P>0.05),抑瘤率为41.23%;分支杆菌Ag85B蛋白治疗组小鼠存活天数为(28.75±2.71) d,显著长于NS组(23.50±2.45) d(P<0.05),与BCG组的(29.63±3.25) d比较差异无统计学意义(P>0.05),存活延长率为22.34%。结论:分支杆菌Ag85B蛋白对小鼠膀胱癌具有抑制作用,并能延长膀胱癌荷瘤鼠的生存期。     

Research of a Novel Ag Temperature Sensor Based on Fabric Substrate Fabricated by Magnetron Sputtering

TPU-coated polyester fabric was used as the substrate of a flexible temperature sensor and Ag nanoparticles were deposited on its surface as the temperature sensing layer by the magnetron sputtering method. The effects of sputtering powers and heat treatment on properties of the sensing layers, such as the temperature coefficient of resistance (TCR), linearity, hysteresis, drift, reliability, and bending resistance, were mainly studied. The results showed that the TCR (0.00234 °C⁻¹) was the highest when sputtering power was 90 W and sputtering pressure was 0.8 Pa. The crystallinity of Ag particles would improve, as the TCR was improved to 0.00262 °C⁻¹ under heat treatment condition at 160°. The Ag layer obtained excellent linearity, lower hysteresis and drift value, as well as good reliability and bending resistance when the sputtering power was 90 W. The flexible temperature sensor based on the coated polyester fabric improved the softness and comfortableness of sensor, which can be further applied in intelligent wearable products.