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51.
目的:选定猕猴桃根粗多糖的脱色材料,确定脱色工艺条件。方法:建立猕猴桃根多糖的含量测定方法,以脱色率为指标,采用单因素试验法,通过优化与比较聚酰胺和DA201型大孔吸附树脂对猕猴桃根粗多糖的脱色作用。结果:聚酰胺对RACP中的色素具有较大的吸附容量和选择性,选定聚酰胺静态吸附法对猕猴桃根粗多糖进行脱色,脱色工艺条件为:多糖浓度2mg/mL,药脂比60mg∶1g,pH5,室温下吸附80min。以该工艺处理猕猴桃根粗多糖,脱色率可达78%,多糖保留率大于85%。结论:建立的猕猴桃根粗多糖的的脱色方法与工艺,简单、可行。 相似文献
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目的:分析对萼猕猴桃叶中总黄酮以及黄酮类成分山柰酚-3-O-β-D-吡喃半乳糖苷(ry-1)、对萼猕猴桃苷E(ry-2)、对萼猕猴桃苷F(ry-3)的含量,为对萼猕猴桃叶品质评价和质量控制提供依据。方法:采用芦丁比色法对对萼猕猴桃叶中总黄酮进行含量分析;建立HPLC法对对萼猕猴桃叶中黄酮类成分ry-1、ry-2、ry-3进行含量分析。色谱柱:Shim-packCLC-ODS色谱柱(4.6mm×25mm,5μm);保护柱:DIKMAEasy-Guard C18(10mm×4.6mm),流动相:乙腈-0.5%醋酸水溶液梯度洗脱;柱温:26℃;检测波长:360nm;流速:1.00mL/min。结果:总黄酮的回归方程为A=8.26×10^-2+1.90×10^-3,r=0.9995,线性范围:0.01~0.09mg/mL;ry-1回归方程为A=6.84×10^-5C+6.53×10^-2,r=0.9999,线性范围:0.792~23.760μg/mL;ry-2回归方程为A=8.58×10^-5C+0.124,r=0.9999,线性范围:2.570~77.100μg/mL;ry-3回归方程为A=7.89×10^-5C+9.54×10-2,r=0.9999,线性范围:2.168~65.040μg/mL。结论:该方法前处理简便,分析准确、快速,可以作为对萼猕猴桃叶中总黄酮以及ry-1、ry-2、ry-3的定量分析方法,也为对萼猕猴桃叶的质量控制和品质评价提供了重要的依据。 相似文献
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《International journal of food sciences and nutrition》2013,64(4):381-394
AbstractThe present research aimed at evaluating the vitamin C, total phenolic content (TPC), phenolic compounds, carotenoids, and chlorophyll contents, as well as antioxidant activity (AAC) of six Actinidia species fruit. Vitamin C, phenolic compounds, carotenoids and chlorophylls were measured using high-performance liquid chromatography. TPC was determined using the Folin–Ciocalteau reagent, and AAC using 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay. The highest concentrations of vitamin C and TPC were found for Actinidia kolomikta fruit (1008.3 and 634.1 mg/100 g fresh weight [FW], respectively). Among phenolic compounds, seven phenolic acids and three flavonoids were identified. The 2,5-dihydroxybenzoic acid prevailed in A. kolomikta (425.54 mg/100 g FW), while tannic acid dominated in other species (4.63–100.43 mg/100 g FW). The largest amounts of chlorophylls and carotenoids were identified as Actinidia macrosperma (4.02 and 2.09 mg/100 g FW, respectively). The AAC of fruit extracts decreased in the order of A. kolomikta >?Actinidia purpurea >?Actinidia melanandra >?A. macrosperma >?Actinidia arguta >?Actinidia deliciosa according to the DPPH assay. 相似文献
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中药猫人参中的抗肿瘤活性成分 总被引:1,自引:0,他引:1
目的对中药猫人参(镊合猕猴桃Actinidia valvataDunn.根)中的抗肿瘤活性成分进行研究。方法运用多种色谱分离方法进行分离纯化,通过1HNMR、13CNMR、MS等波谱技术确定化合物的结构,并采用MTT法测试了部分单体化合物的体外细胞毒活性。结果分离并鉴定了11个化合物,其中10个为三萜类化合物:2α,3α,24-三羟基乌苏烷-12-烯-28-酸(1),积雪草酸(2),科罗索酸(3),2α,3α,23,24-四羟基乌苏烷-12-烯-28-酸(4),2α,3α,24-三羟基乌苏酸-11-烯-13β,28-内酯(5),2α,3α,24-三羟基齐墩果烷-12-烯-28-酸(6),2α,3α,19α,24-四羟基乌苏烷-12-烯-28-酸(7),2α,3α,24-三羟基乌苏烷-12,20(30)-二烯-28-酸(8),2α,3β,24-三羟基乌苏烷-12-烯-28-酸(9),齐墩果酸(10);1个植物甾醇:β-谷甾醇(11)。采用MTT法测试了化合物1、2、3、4、7对A549(人肺癌细胞)、LOVO(人结肠癌细胞)、HepG2(人肝癌细胞)等3种人肿瘤细胞株的体外细胞毒活性。结论化合物3~8为首次从该植物中分离得到,其中2、3对LOVO和HepG2细胞株有一定的抗肿瘤活性,且随着单体化合物极性的增大,细胞毒活性下降。 相似文献
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Objective:To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma(HCC) cells in vitro.Methods:Total saponin was extracted from the root of A.valvata(TSAVD).HCC cells,such as BEL-7402,HepG2,PLC,SMMC-7721,MHCC-97-H, and MHCC-97-L,were treated with TSAVD in 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide(MTT) assay.BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays,and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed.Meanwhile,the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. Results:TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios(IRs) of 61.08%,74.12%, 84.55%at 24,48,and 72 h,respectively.Meanwhile,TSAVD inhibited MHCC-97-H proliferation in a concentrationdependent manner from 1.5 to 0.5 mg/mL,with the IR of 36%at 1.5 mg/mL at 24 h.For SMMC-7721,PLC, and HepG2,the IR was lower than 30%at 1.5 mg/mL at 24 h.In the wound healing assay,mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group.After pretreatment for 24 h with TSAVD,adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells,with IRs of 48.50%±4.86%and 49.85%±5.25%at 200 |xg/mL.The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91%and 79.37%±0.09%at 200μg/mL In the invasion assay,IRs were 69.78%±4.88%and 82.48%±0.25%at 200μg/mL Conclusions:Of all HCC cells,the highest inhibition by TSAVD was seen for BEL-7402 proliferation.TSAVD could restrain adhesion,invasion,mobility,and migration abilities of BEL-7402 and MHCC-97-H cells in vitro. 相似文献