甲状腺肿瘤组织CD15抗原和bcl-2基因蛋白表达的临床意义

目的 :研究甲状腺癌组织中CD15抗原和bcl 2的基因蛋白的表达与肿瘤发生和转移的关系 ,并探讨CD15与bcl 2基因蛋白表达的关系。方法 :应用微波 LSAB免疫组织化学法检测 50例甲状腺癌、4 5例甲状腺腺瘤和 2 0例癌旁正常甲状腺组织中CD15和bcl 2基因蛋白表达。结果 :在甲状腺癌中CD15和bcl 2基因蛋白表达阳性率分别为 6 8 0 %和 4 6 0 % ,均显著高于甲状腺腺瘤和癌旁正常甲状腺组织 (P<0 0 5) ,CD15和bcl 2阳性率均与甲状腺癌淋巴结转移相关 (P <0 0 5) ,与甲状腺癌组织类型无明显关系 (P >0 0 5)。CD15表达与bcl 2表达呈明显正相关性。结论 :CD15和bcl 2表达对预测甲状腺癌转移和评估预后是一种重要参考指标。     

DELETION AND 5'CPG ISLAND METHYLATION OF p15 GENE IN BRAIN GLIOMA

P15geneisananothertumorsuppressorgene,whichislocatedongpZIandadjacenttopl6gene.Itencodesplsproteinthathasbiochemicalfunctionssimilartothoseofp16protein.Therehavebeenreportsaboutabnormalityofplsgeneinbrainglioma.['-']Inthisstudy,wedetecteddeletionand5'CPGislandmethylationofp15genein56casesofbraingliomabythemethodsofPCRandPCR-basedmethylationtoinvestigatethecorrelationbetweenabnormalityofplsgeneandoccurrenceormalignantprogressionofbrainglioma.MATERIALSANDMETHODSSpecimensFifty-sixfre…     

胆囊癌组织CD15和c-erbB-2表达及相关性研究

目的: 研究CD15抗原和c-erbB-2癌基因蛋白产物在胆囊癌组织中的表达与组织类型、病理分级和转移的关系,并探讨CD15与癌基因蛋白表达的相关性。方法: 应用微波-SP免疫组化法,检测45例胆囊腺癌、17例胆囊腺瘤和10例慢性胆囊炎组织中CD15和c-erbB-2的表达水平。结果: 在胆囊癌中CD15和c-erbB--2阳性表达率分别为71.1％和48.9％。其表达阳性率在胆率癌中均明显高于胆囊腺瘤(P<0.05),并与胆囊癌的类型、病理分级和转移密切相关(P<0.05)。CD15表达与c-erbB-2表达呈明显相关联。结论: CD15和c-erbB-2是胆囊癌高度恶性和预后不良重要生物学指标。胆囊癌CD15表达与c-erbB-2蛋白表达具有相互协同作用可能。     

乳腺癌组织中细胞粘附分子CD15的定量研究

为了探讨细胞粘附分子ＣＤ１５表达及含量与乳腺癌分化程度和淋巴结转移状况的关系,应用微波－ＳＰ法和图像分析技术,对９４例乳腺癌和１０例正常乳腺组织中ＣＤ１５的表达及含量进行检测。结果：ＣＤ１５表达阳性物质在正常乳腺中有很极性地位于腺上皮游高面,而在乳腺癌中主要分布于浆膜;ＣＤ１５表达阳性率和图像定量分析平均光密度值在乳腺癌组织中均显著高于正常乳腺组织,并均随乳腺癌分化程度减低和淋巴结转移而     

Genetic deletion of endothelial microRNA-15a/16-1 promotes cerebral angiogenesis and neurological recovery in ischemic stroke through Src signaling pathway

Cerebral angiogenesis is tightly controlled by specific microRNAs (miRs), including the miR-15a/16-1 cluster. Recently, we reported that endothelium-specific conditional knockout of the miR-15a/16-1 cluster (EC-miR-15a/16-1 cKO) promotes post-stroke angiogenesis and improves long-term neurological recovery by increasing protein levels of VEGFA, FGF2, and their respective receptors VEGFR2 and FGFR1. Herein, we further investigated the underlying signaling mechanism of these pro-angiogenic factors after ischemic stroke using a selective Src family inhibitor AZD0530. EC-miR-15a/16-1 cKO and age- and sex-matched wild-type littermate (WT) mice were subjected to 1 h middle cerebral artery occlusion (MCAO) and 28d reperfusion. AZD0530 was administered daily by oral gavage to both genotypes of mice 3-21d after MCAO. Compared to WT, AZD0530 administration exacerbated spatial cognitive impairments and brain atrophy in EC-miR-15a/16-1 cKO mice following MCAO. AZD0530 also attenuated long-term recovery of blood flow and inhibited the formation of new microvessels, including functional vessels with blood circulation, in the penumbra of stroked cKO mice. Moreover, AZD0530 blocked the Src signaling pathway by downregulating phospho-Src and its downstream mediators (p-Stat3, p-Akt, p-FAK, p-p44/42 MAPK, p-p38 MAPK) in post-ischemic brains. Collectively, our data demonstrated that endothelium-targeted deletion of the miR-15a/16-1 cluster promotes post-stroke angiogenesis and improves long-term neurological recovery via activating Src signaling pathway.     

自主性甲状腺结节显像诊断分析

目的评价９９ｍＴｃ、９９ｍＴｃＭＩＢＩ（甲氧基异丁基异腈）核素显像、Ｂ超在自主性甲状腺结节（ＡＦＴＮ）诊断中的应用。方法３１例患者男９例,女２２例,年龄１５～８７岁。全部病例均完成血清ＦＴ３、ＦＴ４、ＴＳＨ的测定,并进行了Ｂ超甲状腺检查、９９ｍＴｃ以及９９ｍＴｃＭＩＢＩ甲状腺显像。结果３１例患者中,２８例９９ｍＴｃ显像表现为单发“热”结节,３例为多发“热”结节,“热”结节周围的甲状腺组织不显像者共１０例,显像较差者２１例。９９ｍＴｃＭＩＢＩ甲状腺显像结果为,１７例“热”结节外原显像较差或不显像的甲状腺组织均获得一定程度改善,另１４例未见明显变化。在伴有典型甲亢症状的１３例中,ＦＴ３及ＦＴ４均高于正常值上限、ＴＳＨ低于正常值下限的９例。另４例表现为Ｔ３型甲亢。在甲亢症状不典型的１８例中,２例诊断为Ｔ３型甲亢,４例为亚临床甲亢;其余１２例甲状腺功能正常。结论９９ｍＴｃＭＩＢＩ甲状腺显像可以代替ＴＳＨ刺激显像或甲状腺抑制显像而用于ＡＦＴＮ的临床诊断     

NOD小鼠口服胰岛素对胰岛Fas及其配体表达的影响

目的观察口服胰岛素对ＮＯＤ小鼠糖尿病和胰岛炎发生情况的影响以及观察口服免疫耐受后胰岛Ｆａｓ和Ｆａｓ配体（ＦａｓＬ）表达的改变情况。方法将雌性ＮＯＤ小鼠６４只,随机分为两组：一组（３０只）给予磷酸缓冲液（ＰＢＳ）５００μｌ,另一组（３４只）给予胰岛素１ｍｇ加ＰＢＳ５００μｌ,５周龄开始给药,第１周两次,以后每周一次至３０周。采用免疫组化染色法观察了口服免疫耐受治疗后ＮＯＤ小鼠胰岛组织Ｆａｓ和ＦａｓＬ的改变情况。结果口服胰岛素能明显减少胰岛炎和糖尿病的发生,对照组１４周龄即出现糖尿病而胰岛素组２６周龄才出现糖尿病,２６周龄时两组的发病率分别为１１％和７９％（Ｐ＜０．００１）,Ｆａｓ出现在糖尿病小鼠而ＦａｓＬ出现在胰岛素喂饲后的小鼠。结论口服胰岛素能明显减少胰岛炎和糖尿病的发生,口服胰岛素诱导的ＦａｓＬ表达在胰岛素阻止糖尿病和胰岛炎的发生机制中起一定的作用     

Interleukin-15 blocks apoptosis and induces proliferation of the human myeloma cell line OH-2 and freshly isolated myeloma cells

The growth factor-dependent myeloma cell line OH-2, which has previously been shown to be responsive to interleukin (IL)-6, tumour necrosis factor (TNF)-alpha and lymphotoxin, was examined for response to other growth factors. Enhanced proliferation was found in the presence of IL-10, IL-15, IL-2 and insulin growth factor (IGF)-1. Proliferation was strongest in response to IL-6, intermediate and roughly equipotent in response to IL-15, IL-10 and TNF-alpha, and modest in response to IL-2 and IGF-1. IL-15 was synergistic with TNF-alpha, whereas combinations of IL-15 and the other cytokines were merely additive. IL-15-induced proliferation could not be blocked by neutralizing antibody against gp 130, the common transducer chain of IL-6 and related cytokines. IL-15 and IL-6 prevented apoptosis equally well, both better than TNF-alpha, IL-10, and IGF-1. In four out of six samples of purified primary cells, IL-15 and IL-6 induced proliferation. Furthermore, IL-15 mRNA was detected by RT-PCR in most myeloma cell lines and freshly isolated purified patient samples. IL-15 protein was detectable only in one out of about 20 tested cell supernatants from patients and myeloma cell lines. The OH-2 cell line is multi-responsive to cytokines and is a good system for the study of integration of cytokine signal transduction and growth control in myeloma. IL-15 represents a novel modality of growth regulation in myeloma.     

Bivalent 15 Regularly Associates With the Sex Vesicle in Normal Male Meiosis

Using fluorescent in-situ hybridization, we investigated the positioning of different human bivalents at the pachytene stage of normal male meiosis. We showed that, in about 35% of nuclei, the pericentromeric region of bivalent 15 is closely associated with the sex vesicle (SV). This behaviour may be linked to the presence of three domains in the pericentromeric region of chromosome 15: a large imprinted domain, a nucleolar organizing region (NOR), and a heterochromatic block. In order to define the domains of chromosome 15 involved in this association, we analysed the meiotic behaviour of other bivalents with similar domains: human bivalent 11 and mouse bivalent 7, bearing imprinted domains, other human acrocentric bivalents bearing a NOR, and the human bivalents 1, 9 and 16 containing a heterochromatic region. None of these bivalents were as frequently associated with the SV as the human bivalent 15. Nevertheless, we suggest that the bivalent 15 heterochromatin may be responsible for the association because of two properties: its telomeric location on chromosome 15 and its strong sequence homology with the Yq heterochromatin. This phenomenon could explain the high frequency of translocations between the chromosome 15 and the X or Y chromosomes.     

Action-potential-like depolarizations relieve opioid inhibition of N-type Ca2+ channels in NG108–15 cells

 The ability of action-potential-like waveforms (APWs) to attenuate opioid-induced inhibition of N-type Ca²⁺ channels was investigated in the neuroblastoma × glioma cell line NG108–15 using whole-cell voltage clamp methods. In in vitro differentiated NG108–15 cells, the opioid agonist [d-ala²]-methionine-enkephalin (DAME) reversibly decreased ω-conotoxin-GVIA-sensitive Ba²⁺ currents (N-type currents). Agonist-mediated inhibition of N-type currents could be transiently relieved by strong unphysiological depolarizing prepulses to +80 mV (facilitation). Significant facilitation was also achieved by conditioning the cell with a train of 15 APWs, which roughly mimicked physiological action potentials (1- to 6-ms-long depolarizations to +30 mV from a holding potential of –40 mV). The APW-induced facilitation depended on both conditioning pulse frequency and duration. Summation of the disinhibition produced by each APW was possible because reinhibition following repolarization to –40 mV was a much slower process (τ=88 ms) than the onset of facilitation at +80 mV (τ=7 ms). These results provide evidence that N-type Ca²⁺ channel facilitation may be a physiologically relevant process, and suggest that neuronal firing may relieve agonist-induced inhibition of N-type currents to an extent depending on both the shape of action potentials and the frequency of firing. Received: 14 September 1998 / Accepted: 29 September 1998