Retracted: EphB3‐targeted regulation of miR‐149 in the migration and invasion of human colonic carcinoma HCT116 and SW620 cells

microRNAs play key roles during various crucial cell processes such as proliferation, migration, invasion and apoptosis. Also, microRNAs have been shown to possess oncogenic and tumor‐suppressive functions in human cancers. Here, we describe the regulation and function of miR‐149 in colorectal cancer cell lines. miR‐149 expression patterns were detected in human colorectal cell lines and tissue samples, and then focused on its role in regulation of cell growth, migration, invasion, and its target gene identification. Furthermore, the function of the target gene of miR‐149 was analyzed in vitro and in vivo. miR‐149 expression was downregulated in human colorectal cancer HCT116 and SW620 cell lines compared to the normal colon epithelial NCM460 cell line using quantitative real‐time polymerase chain reaction methods. Further studies indicated that introduction of miR‐149 was able to suppress cell migration and invasion. Then, EphB3 was identified as a direct target gene of miR‐149 in colorectal cancer cells. Moreover, experiments in vitro showed that knockdown expression of EphB3 could suppress cell proliferation and invasion, and ectopic expression of EphB3 restored the phenotypes of CRC cell lines transfected with miR149. In addition, silencing of EphB3 significantly affected cycle progression distribution and increased apoptosis in CRC cell lines. Finally, in vivo results demonstrated that knockdown of EphB3 by siRNA inhibited tumor growth. In conclusion,the important role of miR‐149 in colorectal cancer progression suggesting that miR‐149 may serve as a therapeutic target for colorectal cancer treatment.     

延龄草总皂苷调控hsa_circ_0025033/miR-149对非小细胞肺癌细胞生物学行为的影响

目的:研究延龄草总皂苷对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞A549增殖、凋亡、迁移和侵袭的影响。方法:将不同浓度的延龄草总皂苷作用于A549细胞,应用实时定量PCR检测hsa_circ_0025033和miR-149的表达,应用双荧光素酶基因系统验证hsa_circ_0025033与miR-149的靶向关系。分析干扰hsa_circ_0025033表达对A549细胞增殖、凋亡、迁移和侵袭的影响。结果:延龄草总皂苷可抑制A549细胞增殖、迁移和侵袭,诱导细胞凋亡,下调hsa_circ_0025033表达,上调miR-149表达(P＜0.05)。干扰hsa_circ_0025033可抑制A549细胞增殖、迁移和侵袭,诱导细胞凋亡(P＜0.05)。hsa_circ_0025033可靶向结合miR-149,干扰hsa_circ_0025033表达可上调miR-149表达(P＜0.05)。过表达hsa_circ_0025033逆转延龄草总皂苷对A549细胞增殖、凋亡、迁移和侵袭的影响(P＜0.05)。结论:延龄草总皂苷能够抑制NSCLC细胞A549增殖、迁移和侵袭,诱导细胞凋亡,可能与调节hsa_circ_0025033/miR-149通路有关。     

miR-149与肿瘤关系的研究进展

miRNAs是一类长度约为20~25个核苷酸,参与基因调控表达的内源性非编码RNA。miR-149作为miRNAs的重要成员,在多种肿瘤中表达异常,其表达水平与肿瘤细胞增殖、转移、凋亡、耐药、患者的早期诊断及预后密切相关。因此,miR-149有望成为新一类抗肿瘤治疗的靶点。     

miR-149-5p通过靶向AEBP1调控胃癌细胞的迁移侵袭

目的：探讨miR-149-5p对胃癌细胞迁移侵袭能力的影响及分子机制。方法：qRT-PCR检测胃癌细胞株和组织标本中miR-149-5p的表达,分析其表达水平与胃癌患者临床病理特征参数及预后的相关性,建立过表达和干扰miR-149-5p的胃癌细胞株,Transwell实验检测miR-149-5p表达水平对胃癌癌细胞迁移侵袭能力的影响;生物信息学网站预测miR-149-5p的靶基因,采用荧光素酶报告基因实验加以验证。结果：miR-149-5p在胃癌组织和细胞株中均低表达（均P<0.05）。miR-149-5p的表达水平与胃癌患者的浸润深度（P=0.016）、淋巴结转移（P=0.001）和TNM分期（P=0.023）相关。miR-149-5p低表达是胃癌患者总生存期的独立危险因素。与对照组相比,miR-149-5p mimics明显抑制胃癌细胞的迁移侵袭能力（均P<0.01）,而转染miR-149-5p inhibitors后得到了相反的结果（均P<0.05）。miR-149-5p靶向调控脂肪细胞增强结合蛋白1(Adipocyte enhancer binding pro...     

人microRNA149抑制表达慢病毒载体的构建及对黑色素瘤细胞增殖及迁移的影响

目的 构建人microRNA149(Hsa-miR-149)抑制表达慢病毒载体,并探讨抑制miR-149表达对黑色素瘤Mel-RM细胞增殖及迁移的影响.方法 由miRBase及NCBI数据库查找获得miR-149序列,设计合成其引物序列,通过退火反应获得双链DNA寡核苷酸片段,与载体pBS-hU6-1连接,将连接后的载体与FG12连接,经测序鉴定后获得重组慢病毒载体;将重组慢病毒质粒分别与包装辅助质粒共转染至293T细胞中,包装产生病毒,进行病毒滴度测定,再用包装后的病毒感染Mel-RM细胞,利用实时荧光定量PCR法检测miR-149表达水平.同时用MTT法及细胞迁移实验分别检测细胞增殖和迁移能力.结果 成功构建了抑制miR-149表达的慢病毒载体,测序证实了所插入的基因序列正确.在荧光显微镜下观察到转染后的297T细胞表达大量绿色荧光蛋白,收集病毒,用梯度滴定法测得的重组载体病毒滴度为3.2×1010 TU/L.将病毒感染Mel-RM细胞显示可有效降低miR-149的表达水平(P＜0.05).MTT法结果显示与感染空载FG12病毒的对照组相比,感染抑制miR-149表达重组质粒的实验组中活细胞数明显减少(P＜0.05).细胞迁移实验结果显示与对照组相比实验组明显抑制了Mel-RM细胞的迁移(P ＜0.001).结论 成功构建抑制miR-149表达的慢病毒载体,抑制miR-149的表达可以抑制Mel-RM细胞的增殖和迁移.并为后续进一步研究miR-149对黑色素瘤细胞发生发展的影响提供依据.     

Manikin-based size-resolved penetrations of CE-marked filtering facepiece respirators

The purpose of this manikin-based study was to determine the percentage penetrations of nine CE-marked filtering facepiece respirator models (two samples from each) from filtering classes FFP1, FFP2, and FFP3 and to demonstrate by an independent measurement method the disadvantages and shortcomings of the currently valid European Norm (EN 149:2001) for filtering facepieces. All of the filtering facepieces were evaluated size-selectively in an experimental chamber using charge-neutralized monodisperse ammonium sulfate in 9 sizes ranging from 20–400 nm of count median diameter (CMD) under flowrate of 95 L/min. The results were then compared to the previous study concerning penetrations of 47-mm diameter filters cut from the filtering material of identical filtering facepieces. Although these two experimental methods for measuring penetrations of filtering materials from filtering facepieces are in good agreement (R² = 0.91), the results show within-respirator variations in all three filtering classes (5.5–19.3% for all FFRs in FFP1, 2.8–8.5% in FFP2, and 0.1–2.8% in FFP3). The most penetrating particle size (MPPS) in this study was found to be in the range of 25–65 nm (CMD), which is in agreement with the range of 30–60 nm found in the previous study. Moreover, 7 out of 9 FFR models reached higher penetrations from manikin-based respirator measurements than during measurements of filters from the respective respirators. Furthermore, penetration levels increased up to ～50% when the respirator was not sealed around the face of the manikin, indicating that the real protection level provided by these filtering facepieces may be even lower if the respirator does not fit perfectly. Considering that poor filtration efficiency and poor fit may increase under real work conditions, the particle penetration is even higher than was found in this study. Therefore, the CE-marked respirators examined in this study may not be efficient in providing the expected level of protection for workers exposed to nanoparticles.     

Low MiR-149 expression is associated with unfavorable prognosis and enhanced Akt/mTOR signaling in glioma

microRNAs (miRs) play critical roles in the progression of glioma. Previous in vitro studies have described the anti-tumor role of miR-149 in cancer cells including glioma. In this study, we aimed to investigate whether miR-149 is associated with the prognosis of glioma patients. A total of 163 glioma patients who underwent tumor resection were included in our follow-up study. We found that the miR-149 expression was significantly lower in tumor tissues compared with that in normal tissues (P<0.05). Kaplan-Meier and analysis showed that the miR-149 expression status was significantly associated with the survival duration (logrank test, P<0.001), and multivariate Cox regression revealed that patients with low miR-149 expression were exposed to a 1.825 fold higher death risk (HR=1.825, 95% CI=1.031-3.229, P=0.039) compared with those with high miR-149 expression. Further study showed that Akt/mTOR signaling was hyperactive in low miR-149 expressing tissues. Our study thus demonstrates that miR-149 expression in glioma tissues is critically associated with the prognosis of patients, suggesting its potential clinical significance.     

基于Android系统的心电传输与显示系统设计

目的:设计一种基于Andriod平台的心电监护、无线传输与手机显示系统,可用于家庭或社区范围内的心电监测.方法:首先通过心电采集模块提取被监测者的心电信号,然后利用MSP430F149单片机进行A/D转换并将转换后的数据文件适当处理后经串口发送至蓝牙模块,再无线传输至智能手机,存储在手机的安全数码卡(secure digital memory card,SD)上.智能手机端通过设计蓝牙的应用程序编程接口(application programming interface,API)开发类实现与下位机通信,可打开已存储的心电数据文件并绘制成心电波形图,观察心电图是否有明显异常.结果:该系统能正常采集到人体心电信号并无线传输至手机进行显示,无线传输距离为10 m.结论:该系统的设计有利于家庭其他成员或监护人员及时了解患者的心电状况,以实现对家庭患者的健康监护.