桃核承气胶囊治疗高半胱氨酸血症40例

已有研究证明,10％的冠状动脉疾病与血浆同型半胱氨酸(以下简称半胱氨酸)有关,半胱氨酸浓度升高与冠心病有密切的关系,是心血管病新的独立危险因素。我们运用桃核承气胶囊治疗高半胱氨酸血症,取得了较好疗效,现报告如下。1 临床资料 1999年10月～2000年3月确诊为冠心病、抽血检查半胱氨酸高于正常的住院患者40例,其中男性24例,平均年龄67.32±6.42岁,女性16例,平均年龄67.52±3.93。选择血浆     

一种新的细胞凋亡相关基因——高同型半胱氨酸诱导基因HCY-2的功能初探

目的　探讨高同型半胱氨酸 (HCY)血症引起心血管病和出生性缺陷的作用机制。方法　采用诱导筛选方法克隆高同型半胱氨酸诱导基因 HCY- 2 ,以 Northern印迹分析检测 HCY- 2基因在大鼠不同组织中的表达 ,以免疫组化方法验证 HCY- 2蛋白在大鼠不同组织中的表达。结果　以诱导筛选方法从大鼠血管平滑肌细胞内克隆到一个新的全长 c DNA,即高同型半胱氨酸诱导基因 HCY- 2 ,它编码 142个氨基酸。Northern印迹分析和免疫组化检测表明 ,HCY- 2基因可在大鼠心、肾、脑、肝、肺等组织中广泛表达。在体外 ,将重组 HCY- 2基因转移至内皮细胞中 ,能够引起细胞凋亡和 DNA损伤 ;在体内 ,将 HCY- 2基因转移至鸡胚内 ,则诱发鸡胚细胞凋亡 ,并引起畸形。结论　 HCY- 2基因可能是一种新的凋亡基因 ,高同型半胱氨酸血症可能通过 HCY- 2基因诱发心血管病和出生性畸形。     

输血浆也需要血型相同吗？

前不久，一个病人需输血浆，其亲人想给他提供，他是AB型血，而亲人是O型血，病人对于我们要给病人输同型血浆很困惑，他们认为血浆里没红细胞不会发生溶血反应，那么输血浆为什么也要同型呢？     

鼻腔鼻窦鳞状细胞癌中细胞质凋亡抑制蛋白2和半胱氨酸蛋白水解酶4的表达

目的通过检测细胞质凋亡抑制蛋白2(cytoplasmic Inhibitor of apoptosis protein 2,c-IAP2)和半胱氨酸蛋白水解酶4(caspase-4)在鼻腔鼻窦鳞状细胞癌(简称鳞癌)组织中蛋白质水平的表达情况,研究其表达的相关性,探讨此2种凋亡相关基因控制蛋白在鼻腔鼻窦鳞癌发生、发展中的作用和机制.方法选取已确诊的41例鼻腔鼻窦鳞癌患者的标本为实验组,10例正常鼻黏膜标本为对照组,以免疫组化法检测caspase-4和c-IAP2在鼻腔鼻窦鳞癌中的表达,光镜下计数.结果①c-IAP2在41例鼻腔鼻窦鳞癌患者中36例阳性表达,阳性率为 87.8%, 其中14例表达(+++),15例(++),7例(+),5例表达缺失(-),与对照组相比差异有显著性(实验组平均秩=29.11,秩和T1=1194.00,对照组平均秩=13.20,秩和T2=132.00,统计量Z=-3.141,双侧渐近概率为0.0017),且c-IAP2的表达与临床分期无关(P>0.05),与病理分期(P<0.01)及临床预后(P<0.01)有相关性;②caspase-4在41例鼻腔鼻窦鳞癌患者中17例表达缺失(-),13例低表达(+),与对照组差异有显著性(实验组平均秩=23.76,秩和T1=974.00,对照组平均秩=35.20,秩和T2=352.00,统计量Z=-2.267,双侧渐近概率为0.0277),且caspase-4的表达与临床分期无关(P>0.05),而与病理分期(P<0.01)及临床预后(P<0.01)有相关性;③caspase-4与c-IAP2的表达具有一致性,两者的表达呈负相关关系.结论在鼻腔鼻窦鳞癌组织中,c-IAP2被激活而caspase-4被抑制,凋亡通路被阻断,导致了肿瘤细胞的不死性,与鼻腔鼻窦鳞状细胞癌的发生发展应有密切关系,并且可能与部分肿瘤的放射治疗和化学治疗失败有关.     

鼻腔鼻窦鳞状细胞癌中细胞质凋亡抑制蛋白2和半胱氨酸蛋白水解酶4的表达

OBJECTIVE: To examine the expression of apoptosis-related genes c-IAP2 and caspase-4 in sinonasal squamous carcinoma. To investigate the role and mechanism of the two apoptosis-related genes in nasal cavity and sinus squamons carcinoma. METHODS: Forty-one pathologically confirmed specimens of nasal cavity and sinus squamons carcinoma were subjected for immunohistochemical staining to analyze the expression of c-IAP2 and caspase-4, in addition 10 normal nasal mucosa were used as control. RESULTS: The positive rate of c-IAP2 in sinonasal squamons carcinoma was 87.8%, among which 14 cases were strongly stained(+ + +), 15 cases were stained(+ +), 7 cases were stained (+), only 5 cases were negative. In 10 normal control tissues, no case was stained(+ + +), 2 cases were stained (+ +), 3 cases were stained(+), the other 5 cases were negative. Higher level expression of c-IAP2 protein was detected more often in squamons carcinoma specimens than that in normal nasal mucosa. There was statistically significant difference between the two groups for the staining(P < 0.05). The expression of c-IAP2 was not correlated with clinical staging (P > 0.05), but there were positive correlation with clinical prognosis (P < 0.01) and pathological classification (P < 0.01); The positive rate of caspase-4 in nasal cavity and sinus squamons carcinoma was 58.5%, among which 4 cases were strongly stained (+ + +), 7 cases were stained (+ +), 13 case was stained(+), 17 cases were negative. The expression of caspase-4 protein was more deletion in squamons carcinoma specimens than that in control group, there was statistically significant difference between the two groups for the staining (P < 0.05). The expression of caspase-4 protein was negatively correlated with clinical prognosis (P < 0.01) and pathological classification ( P < 0.01), not correlated with clinical staging(P > 0.05). The expression of c-IAP2 was negativelly correlated with the experssion of caspase-4. CONCLUSIONS: The results indicates that in sinonasal squamous carcinoma, c-IAP2 is activated, while caspase-4 is restrained, the signal transduction pathway of apoptosis is arrested, leading to surviving of tumor cells, this maybe directly associated with the development of sinonasal squamons carcinoma, and with the failure of tumor trentment.     

从中国期刊网全文数据库检索效果谈检索的方法

《中国期刊全文数据库(CJFD)》收录了1994年以来我国公开出版发行的学术类核心期刊与专业期刊6100余种，分九大专辑，126个专题文献数据库，数据完整性达到98％，所囊括的资源总量达到全国同类资源总量的80％以上，成为一种与印刷型期刊同步出版的大型集成化动态全文信息资源。它提供的最基本的检索方法有：全文、中文文摘和关键词、英文文摘和关键词、篇名、作者、机构、刊名、     

高同型半胱氨酸血症与高血压

同型半胱氨酸(hornocysteine，hcy)是一种含硫氨基酸，是蛋氨酸循环的重要中间代谢产物。流行病学调查、临床和实验研究表明，高同型半胱氨酸血症【perhornocysteinernia，hh(e)】与冠状动脉疾病、脑血管疾病、外周血管疾病密切相关。近年来随着对其研究的深入，一些学者发现血hcy水平升高伴有血压升高，hh(e)和高血压可能存在相关性。现就血浆hcy浓度升高的原因，hh(e)和高血压的相关性及其可能机制进行综述。     

重视维持性血液透析病人的营养指导

关键词：维持性透析 营养 肾功能衰竭 营养不良是维持性血液透析(简称血透)病人死亡的主要因素之一。为此，我们十分重视对维持性血透病人的营养状况。从1999年2月以来，重点对维持性血透病人的营养状况进行了跟踪观察，针对引起营养不良因素进行个体化指导，收到了良好效果。具体指导原则介绍如下。