放射线联合p53基因及内皮抑素治疗小鼠前列腺癌皮下移植瘤

Objective To test the hypothesis that p53 gene therapy combined with endostatin can enhance tumor response to radiation therapy of RM-1 mouse xenograft prostate cancer and to investigate its mechanism. Methods A mouse prostate cancer model was established. Then mice with xenograft tumor were randomly divided into group A (control), B (radiation), C (radiation and rAdp53), D (radiation and rh-endostatin) and E (radiation and rAdp53 and rh-endostatin). On day 1, rAdp53 was injected intra-tumorously with 1 × 1010 vp per animal to group C and E. From day 1 to 14, rh-endostatin was given 15 mg/kg intraperitoneally daily to group D and E. On day 4 single fraction of 15 Gy was given to tumors in groups B, C, D and E. Normal saline was injected intra-tumorously or intraperitoneaUy accordingly as control. No treatment was done to group A. Tumor volume was measured daily. Samples were collected on Days 5, 10 and 15. Ki67, CD31, p53 and VEGF were detected by means of immunohistochemistry. Results (1) Radiation alone, radiation combined with intra-tumorous injection of Adp53 and/or intraperitoneal injection of rh-endostatin resulted in tumor growth arrest of RM-1 cells in vivo (P = 0.000). Radiation combined with both rAdp53 and rh-endostatin was the most effective treatment (P < 0.05). (2) All the four treatment groups had a decreased expression of mutant type P53 (P = 0.000). The expression of Ki67 in groups B and C were equal (P 0.05) and increasing (P = 0.000), respectively. Group D had a up-down-up curve (P < 0.05), but group E had a up-down one. On day 5 the expresion of VEGF in group E was the lowest (P < 0.05). An increased expression of MVD compared with the control was shown, and MVD in groups C, D and E were always higher than that in the control (P < 0.05). Conclusions The limitation of radiotherapy could be overcome by combination with beth p53 gene therapy and endostatin on the growth of mouse prostate cancer cell. Radiation, rAdp53 and endostatin have their own role but they can be interacted with each other.     

瑞芬太尼对老年患者麻醉诱导时血流动力学的影响

［摘要］目的观察瑞芬太尼全麻诱导对老年患者血流动力学的影响。方法60例ASAⅠ～Ⅱ级老年患者随机分成3组，F组、R1组和R2组，每组20例。F组静脉诱导用药：咪达唑仑0.05 mg·kg-1，芬太尼3 μg·kg-1，丙泊酚1 mg·kg-1，待入睡后注入维库溴铵0.1 mg·kg-1气管插管；R1组用瑞芬太尼1 μg·kg-1代替芬太尼；R2组用瑞芬太尼1.5 μg·kg-1代替芬太尼。观察诱导时血压、心率的变化，同时记录使用阿托品和麻黄碱情况。结果R2组最低血压（MAP1 ）、最高血压（MAP2）明显低于其他两组(P＜0.05＝； R2组 最低心率（HR1）、最高心率（HR2）明显低于F组(P＜0.05＝。结论瑞芬太尼可以用于老年患者的全麻诱导，但在诱导时要密切注意血流动力学变化。     

本刊对文稿中关键词的标引要求

关键词是科技论的献检索标识，是表达献主题概念的自然语言词汇。     

关键词

关键词是为了便于编制文献索引、检索和阅读丽选取的能反映文章主题概念的词或河组。本讨所刊发的每篇论文须选取4—8个关键词。     

《职业与健康》杂志来稿撰写要求

1 资料可靠、数据准确、文字精练。论著性稿件请附中英文摘要及2～8个关键词。论文摘要须按结构式撰写，包括目的（Objective）、方法（Methods）、结果（Results）、结论（Conclusion）4个层次，结果中要有主要数据，并列出4个小标题。     

本刊对中英文摘要的写作要求

论著正文前附300字内的中文摘要，包括目的、方法、结果、结论四要素，关键词3～8个；英文摘要应比中文摘要详细，300个实词左右。英文摘要的目的部分应概括写出本研究的研究背景和目的；方法部分应写明研究对象的数目、来源、分组情况及所用的研究方法和工具；结果部分应包括研究结果的主要形态学改变、染色的具体形态和具体数据。英文摘要的方法和结果部分内容的描述应用一般过去时态。英文关键词3～8个，应与中文关键词一致。     

抑素的研究进展

 抑素是一类细胞特异性的有丝分裂抑制因子，存在于动物的多种组织中。由于组织来源、提 取和纯化方法的不同，对其化学本质和理化性质、作用机理和生物活性等方面存在不同观点。本文以淋 巴细胞抑素为重点就其研究概况进行综述，并展望其应用前景。     

补肾活血法治疗感音神经性耳聋的研究概况

综述近１０年来国内应用补肾活血法治疗感音神经性耳聋在基础研究、临床研究、实验研究方面的进展，并指出了现有的研究所存在的不足之处，认为补肾活血法对某些类型的感音神经性耳聋可能有一定的治疗作用，但仍有待于进一步的研究证实。     

健康教育对哮喘儿童情绪及哮喘疗效的影响

糖尿病（DM）是一组不同病因的内分泌代谢性疾病，以高血糖为共同特征，容易并发心血管疾病和糖尿病性肾病。作者通过对DM患者超敏C-反应蛋白（hs—CRP）、脂类、胱抑素C（CysC）、mALB的检测，旨在探讨DM患者血清hs-CRP、CysC的含量与发生并发症的趋势的关系为治疗DM、预防其并发症的发生提供理论依据。