异氟烷催眠、镇痛作用与NMDA受体甘氨酸位点的关系

目的分析异氟烷催眠、镇痛作用与NMDA受体甘氨酸位点的关系。方法建立小鼠异氟烷注射催眠、镇痛模型后,在小鼠催醒、甩尾、福尔马林实验中,观察侧脑室或鞘内注射NMDA受体甘氨酸位点的激动剂D-丝氨酸(D-Serine,D-Ser)后小鼠睡眠时间、甩尾潜伏期或累计舔足时间的变化;用免疫组化方法观察异氟烷及鞘内用药对福尔马林小鼠脊髓Fos蛋白表达的影响。结果侧脑室注射D-Ser对异氟烷的催眠时间无影响(P>0.05)。鞘内注射D-Ser(0.025、0.05、0.1ng)可拮抗甩尾实验、福尔马林实验Ⅰ相中异氟烷的镇痛作用(P<0.05,P<0.01),而对福尔马林实验Ⅱ相异氟烷的镇痛作用无影响(P>0.05)。鞘内注射D-Ser0.05ng可拮抗异氟烷对福尔马林小鼠脊髓Fos蛋白表达的抑制作用(P<0.01)。结论异氟烷催眠作用与脑内NMDA受体甘氨酸位点关系不大;脊髓NMDA受体甘氨酸位点介导异氟烷对热、化学刺激的镇痛作用,而与异氟烷对慢性炎性疼痛的镇痛作用无明显关系。     

喷砂机的结构原理、使用与维修

喷砂机是气体动力输送砂粒做功的设备，在口腔牙科医疗中有着十分重要的应用，在口内医护人员对患者进行超声洁牙后，常常用喷砂的方法对牙齿表面进行抛光处理，以巩固牙齿治疗效果。在口腔修复中，牙科技工在铸造烤瓷牙时，喷砂抛光更是必不可少的程序之一。     

喷砂洁治术应用体会

洁治术是彻底清除菌斑和牙石的有效方法。由于洁治后牙面粗糙或有划痕，所以必须抛光。采用喷砂洁牙，不仅可以去除色素，而且可以使牙面光滑，以延缓菌斑和牙石的附着。单纯的超声波龈上洁治术能够有效地去除牙面上的结石和牙菌斑。但是洁治器的工作头在牙面上划过时，在釉质表面留有划痕，造成牙面粗糙不平，从而易使菌斑牙石色素较快沉积，影响疗效保持。     

谷丙甘氨酸胶囊治疗慢性非细菌性前列腺炎86例

慢性非细菌性前列腺炎好发于中青年,临床常见,治疗比较困难.我院于2007年9月-2007年12月应用谷丙甘氨酸胶囊治疗非细菌性前列腺炎86例,效果良好,现报道如下.     

使用气流对牙面光洁度影响

目的：探讨洁治和气流（Air-Flow）技术配合使用去除牙面色素、菌斑方法和对牙面光洁度的影响。方法：是光波干涉显微镜观察36颗离体牙在使用Air-Flow技术后牙面粗糙度的变化，并研究磨光糊剂对减轻其粗糙度的作用。结果：气流喷砂和超声洁治一样会使牙面粗糙度显著增加（P〈0.01），进一步磨光可使牙面粗糙度显著下降。结论：临床上使用Air-Flow后，需要用进口抛光糊剂磨光来增加牙面的光洁度。     

Effects of glycine and methylprednisolone on hemorrhagic shock in rats

Background Methylprednisolone (MP), a synthetic glucocorticosteroid, has been broadly studied in experiments on endotoxin-induced shock and septic shock. This study was designed to ascertain whether glycine and MP can protect against organ injury and death caused by hemorrhagic shock, and to elucidate the underlying mechanisms of these protective effects in rats.Method To establish a shock model, Wistar rats were bled to maintain mean arterial pressure at 30-50 mmHg for 1 hour and subsequently resuscitated with the shed blood and normal saline. Just prior to resuscitation, the rats were randomly assigned to four groups: sham group (operation performed without inducing shock), shock group, shock+glycine group (glycine injected at the beginning of resuscitation) and shock+MP group (MP injected at the beginning of resuscitation).Results ① Seventy-two hours after resuscitation, the survival rate of rats from the shock group had decreased to 20%, while the survival rates of rats from the shock+glycine and shock+MP groups were 77.8% and 80%, respectively. The difference was significant (P&lt;0.05). ② Eighteen hours after resuscitation, pathological alterations in the organs of the rats were apparent. In rats from the shock group, edema, interstitial leukocyte infiltration, and cellular degeneration occurred in the liver, lungs, kidneys, and heart. Glycine and MP reduced these pathological changes significantly. ③ Eighteen hours after resuscitation, the levels of creatine phosphokinase, transaminases, and creatine were elevated significantly in rats from the shock group, indicating injury to the heart, liver, and kidneys, while these levels were elevated only slightly in the shock+glycine and shock+MP groups. The differences were significant (P&lt;0.01). ④ There were significant increases in intracellular calcium and production of tumor necrosis factor (TNF-α) by isolated Kupffer cells stimulated by endotoxin after hemorrhagic shock. These changes were completely prevented by glycine and MP (P&lt;0.01). Conclusion Glycine and MP reduce organ injury and mortality caused by hemorrhagic shock by preventing increase of intracellular calcium levels in Kupffer cell, suppressing Kupffer cell activation, decreasing the production of TNF-α by Kupffer cells, and blocking systemic inflammatory responses.     

1.5%甘氨酸冲洗液——泌尿系内窥镜术中的新型冲洗剂

甘氨酸冲洗液(Glycine Irrigation)是一种非离子性制剂,又是一种泌尿系内窥镜手术中配套的无菌冲洗剂。 本品是按美国 Travenol公司产品“Glycine UrologicalIrrigating Solution”处方,配制成1.5％甘氨酸冲洗液,用2000ml塑料袋包装并配有专用导管,供泌尿系内窥镜术中临床冲洗用。 近20多年来,电子技术的进步,带动了内窥镜技术的发展。内窥镜手术在临床广泛应用,它对减少患者病痛及早日康复具有深远意义,也深受患者欢迎。泌尿系内窥镜手术必须在配套冲洗液下进行,这是不同于其它内窥镜的差异,过去用5％葡萄糖注射液或甘露醇等渗溶液作冲洗剂,在手术视野,残留物清除和对组织损伤等方面带来了一定影响,因此,对泌尿系内窥镜电切手术冲洗液提出特殊的要     

对钴铬合金舌侧翼板粘结桥的粘结面处理的研究

目的：通过对粘结桥的粘结面不同处理的粘结强度测试,为临床应用提供理论依据。方法：选用钴铬合金,铸造成一定规格的（１０ｍｍ×１０ｍｍ×１ｍｍ）板,随机分成５组,以电蚀、喷砂方法处理,用高真空喷镀仪及万能金相显微镜观察,抗剪粘结强度测试,并作统计分析。结果：抗剪粘结强度由强到弱依次为：单纯电蚀;喷砂后电蚀;单纯喷砂;电蚀后喷砂;磨光。结论：对制作钴铬合金舌侧翼板粘结桥的粘结面以电蚀处理为首选;而单纯喷砂处理亦可提高粘结强度,为临床应用提供了方便、易操作的方法。     

纤维蛋白单体的制备及其引起细胞伸展的分子机理分析

目的：探讨纤维蛋白单体引起细胞伸展的机理。方法：通过２ｍｏｌ／ＬＮａＢｒ溶解纤维蛋白制成纤维蛋白单体。采用细胞粘附及抑制方法观察细胞伸展状况。结果：人神经胶质瘤细胞不能在固定的纤维蛋白原上伸展,但能在纤维蛋白单体上伸展,此伸展过程不能被外来的纤维蛋白原、片段Ｅ和Ｄ抑制,但能被纤维蛋白单体和ＲＧＤ（精氨酸－甘氨酸－天冬氨酸）序列所抑制。结论：纤维蛋白单体引起细胞伸展的可能机理为纤维蛋白单体α链上的两个ＲＧＤ序列Ａα（９５－９７）和Ａα（５７２－５７４）暴露,从而促使纤维蛋白单体与细胞作用,调节细胞伸展