小儿肺燥脾湿咳喘论治

肺燥脾湿咳喘大多因外感时燥，引动湿痰趋向燥化而呈现燥湿并存。临床既可表现为干咳无痰，也极易出现稠痰内恋，紧粘气道咳出不畅，痰粘气逆，甚至形成假性嘶喘喉头炎．治疗先以气雾剂吸入潮润气道，湿化燥痰，继用喻氏清燥救肺汤加战润燥化痰，止咳平喘。     

内镜下不同治疗方法对消化性溃疡出血的疗效分析

目的 观察不同治疗方法对消化性溃疡出血的疗效。方法 采用1：10000肾上腺素黏膜下注射、止血夹钳夹血管、速溶型纤维蛋白胶封闭组织的方法，对消化性溃疡进行止血。结果 1：10000肾上腺素组对Ⅰa、Ⅰb、Ⅱa类止血有效率分别为88．9％、100％、100％：止血夹对Ⅰa、Ⅰb、Ⅱa类止血有效率分别为100％、90．3％、90．0％；速溶型纤维蛋白胶对Ⅰa、Ⅰb、Ⅱa类止血有效率分别为50．0％、100％、100％。结论 应根据病情特点采取不同的止血方法。     

导管截流夹在引流袋固定中的应用

临床工作中对术后置引流袋患者进行护理时，一般采用别针将引流袋固定或悬挂于患者床边。但患者在活动或引流量增多时易发生固定物脱落导致意外事故发生。鉴此，笔者采用一次性静脉营养输液袋上的截流夹固定引流袋或夹闭引流管，即在配完营养液后，取下导管截流夹，将引流袋挂于截流夹上然后夹在床单上；此外，还可用于拔除引流管前的关闭试验，如将截流夹置于胃管、T型管及导尿管等，以确定患者是否拔管。本方法具有就地取材、操作简单、固定牢固，经济实用的优点。     

靶向性蛋白激酶B1和环氧合酶-2 shRNA腺病毒载体的构建和在人胃癌细胞的表达

Objective To construct a short hairpin RNA (shRNA) adenovirus vector targeting protein kinase BI (PKB1/Akt1) and cyclooxygenase-2 (COX-2) and observe their expression in human gastric carcinoma cell line SGC-7901. Methods Akt1 and COX-2 shRNA expression frames were sub-cloned to pGSadeno adenovirus vector by homologous recombination technology to construct pGSadeno-Aktl + COX-2 ( pGSadeno-A + C) vector. Furthermore after screening and amplification,recombinant ade-novirus vector was digested with Pacl and transfected into HEK293 cells. The replication adenovirus rAd5-A + C was packed and amplified in the HEK293 cells, and its titer was detected. After human SGC-7901 cells in vitro were transfected by rAd5-A + C,Akt1 and COX-2 mRNA and protein expression levels were detected by real-time PCR and Western blot respectively. Compared with rAdS-A + C,SGC-7901 and gen-eral rAd5-HK were selected as the negative controls. Results The recombinant adenovirus rAd5-A + C was constructed successfully and its titer reached 1.0 ×1010 pfu/ml. Aktl and COX-2 mRNA expression was downregulated significantly, and their ACt values ( 12.26±0.05 and 5.41±0.09 respectively ) were higher than rAd5-HK group (10.63±0.02 and 3.75 +0.08 respectively) and control group (10.57± 0.02 and 3.73±0.08 respectively) (P <0.01 ). There was no significant difference between rAd5-HK and control groups (P >0.05). Aktl and COX-2 protein expression was downregulated by 70.5% and 63.7% respectively ( P < 0.01 ) in rAd5-HK group as compared with control group ( P > 0.05 ). Conclu-sion The shRNA aclenovirus vector targeting Akt1 and COX-2 can specifically inhibit Akt1 and COX-2 expression,and this may be a new strategy in gastric carcinoma gene therapy targeting Akt1 and COX-2.     

腹腔镜胆囊切除术后钛夹在腹腔内异位的观察

目的　观察腹腔镜胆囊切除术 (LaparoscopicCholecystectomy ,L .C)后钛夹在腹腔内异位。方法　前瞻性对2 0 0 2年 1～ 5月间 6 0例L .C病人进行追踪观察临床表现 ,采用B超、腹部X线检查等方法 ,观察钛夹位置的变化与肝外胆管的关系。结果　 6 0例L .C后病人中 5例钛夹发生位置改变。结论　①钛夹在体内 ,对胆道无压迫、无胆漏 ;②观察钛夹异位 ,以采用B超为最简单、经济的方法。     

多层螺旋CT三维重建在左肾静脉压迫综合征中的临床应用

目的探讨多层螺旋CT(MSCT)三维重建在左肾静脉压迫综合征中的诊断价值。方法应用16层螺旋CT对11例左肾静脉压迫综合征病例进行泌尿系增强多期扫描,并获得各种后处理图像。结果11例左肾静脉压迫综合征病例均可见肠系膜上动脉(SMA)压迫左肾静脉(LRV)的征象。CT多平面重建(MPR)直观地显示了腹主动脉(AO)与SMA之间的夹角(α),最大为21.88,°最小为12.86°,平均为17.95°。CT横断位图像显示扩张的左肾静脉直径为12~15.3 mm,平均为13.26 mm;LRV受SMA压迫狭窄处直径为3.0~4.0 mm,平均为3.26 mm。左侧卵巢静脉或睾丸静脉直径为2.1~2.6 mm,平均为2.2 mm。5例增强扫描肾实质期(后期)显示双肾实质密度相差20 HU以上。结论MSCT在胡桃夹综合征诊断中具有明显的优势。     

偏方治秋后燥咳

生姜茶：生姜洗净切10片，茶叶7g，共煮成汁饮服。可发汗解表、温肺止咳，对流行性感冒、咳嗽颇有疗效。     

微形弹簧夹根管安全链的研制

目的：研制一种简单实用的根管安全链——微形弹簧夹根管安全链。方法：针对传统安全使存在的问题，结合临床实际要求及可行性进行设计，对其构造、使用方法及原理进行详细分析，并作进一步的临床评价。结果：微形弹簧夹根管安全链能够有效防止器械落入消化道及呼吸道。结论：使用微形弹簧夹根管安全链可以对根管治疗尤其是磨牙根管治疗提供可靠的安全保障。     

桑菊饮组方特点与应用刍议

桑菊饮为吴瑭所创,首见于<温病条辨>,其组成为"桑叶二钱五分,菊花一钱,连翘一钱五分,薄荷八分,生甘草八分,苇根二钱,杏仁二钱,苦桔梗二钱,水二杯,煮取一杯,日二服"(注:1钱≈3g,1分≈0.3g),用其治疗风温轻证.风温致病多由口鼻而入,肺主皮毛,鼻通于肺,风温病多从肺卫起病.风热是风温病的致病因素,风热易化燥伤阴.吴瑭日:"治上焦如羽,非轻不举."故治风温轻证当疏风透表,宣肺止咳.桑菊饮用轻量辛凉之品疏风透表,宣肺止咳,体现了"治上焦如羽,非轻不举"这一观点.     

桑杏汤加味治疗温燥咳嗽88例临床体会

目的：观察中药桑杏汤加味对温燥咳嗽的治疗作用。结果：全部治愈。结论：桑杏汤加味治疗温燥咳嗽疗效满意。