泌尿腔镜下甘露醇冲洗液的改进

在泌尿腔镜下经尿道前列腺电切术、膀胱肿瘤电切术和膀胱内腺病切除术等 ,膀胱内冲洗液压力的大小、流速是否均匀合适 ,都会直接影响手术野操作的清晰度 ,是手术能否顺利进行的关键所在 ,所以准备充足的膀胱冲洗液是电切手术成功必不可缺少的重要因素。1　制作过程　　在制剂室的无菌间 ,采用密闭式一次性使用静脉营养输液袋灌装 3 %甘露醇 3 0 0 0ｍｌ,根据电切手术的大小 ,预计手术中使用量的多少 ,决定一次灌装数量。2　使用方法　　经尿道电切术中 ,使用 3 %甘露醇冲洗液进行持续性的膀胱冲洗。过去使用的冲洗液为 2 0 %甘露醇加蒸馏水…     

黄药子醇提物不同处理方式对肝毒性的影响

目的:探讨不同极性黄药子醇提物组分导致肝毒性的强弱.方法:将黄药子75%乙醇提取物依次用氯仿、乙酸乙酯、正丁醇萃取,采用SD雄性大鼠进行体内筛选,观察不同部位肝毒性的大小,以血清酶学进行考查.结果:氯仿部位肝毒性作用最强,乙酸乙酯部位次之.结论:黄药子中弱极性化合物组分具有显著的肝毒性.     

丹参注射液合参脉注射液治疗心悸78例临床分析

目的：研究丹参注射液和参脉注射液对心悸的治疗效果。方法：以丹参注射液和参脉注射液对门诊78例心悸进行治疗观察。结果：患者治愈54例,好转21例,无效3例。结论：丹参注射液和参脉注射液对心悸病人治疗有效,值得推广。     

直肠癌术中腹腔冲洗液脱落细胞学临床研究进展

直肠癌术后局部复发、盆腔种植转移成为直肠癌术后主要的致死原因。国内外报道直肠癌根治术后复发率为10％～30％，为预防直肠癌术后复发、转移，国内外学者提出了许多有效的方法如全直肠系膜切除（TME），新辅助化疗，术中腹腔冲洗等。其中术中腹腔冲洗作为手术无瘤技术的重要措施已在临床广泛应用并取得明显的成效。在进行腹腔冲洗达到减瘤的目的的同时，术中腹腔冲洗液脱落细胞学的研究渐成热点，现将国内外有关直肠癌术中腹腔冲洗液脱落细胞学的研究综述如下。     

参白注射液对病毒性心肌炎穿孔素表达的影响

观察了参白注射液对VMC感染模型——体外培养大鼠心肌细胞感染柯萨奇B3(CVB3)穿孔素表达的影响，通过分析其表达水平，旨在探讨参白注射液的作用机制，为临床治疗VMC提供实验依据。     

Effect of curcumin on nitric oxide synthase expression in Iipopolysaccharide-activated microglia cells and the anti-oxidative effect of curcumin

BACKGROUND: It has been demonstrated that curcumin can increase the activities of various anti-oxidase in blood and tissue, effectively eliminate various free radicals, reduce the production of peroxisome, and alleviate oxidative stress reaction. Whether it has the same effect on microglia? OBJECTIVE: To observe the effects of curcumin on the expressions of inducible nitric oxide synthase (iNOS), nuclear factor-κB (NF-κB), and superoxide dismutase (SOD) in microglial cell line BV stimulated by lipopolysaccharide (LPS). DESIGN: An observational comparative study. SETTING: Research Room of Biochemistry, Medical College of Nantong University. MATERIALS: Mice microglia cell line BV, iNOS and NF-κB reporter gene plasmids were presented by Dr. Bhat.NR. from the Medical University of South Carolina (USA). Curcumin was produced by the Xi'an Branch of China Chengdu Scholar Bio-Tech. Co.,Ltd.; LPS (E.Coli O26:B6), anti-mice iNOS monoclonal antibody, horseradish peroxidase labeled goat-anti-mice IgG were the products of Sigma Company (USA). METHODS: The experiments were carried out in the Research Room of Biochemistry, Medical College of Nantong University from May 2006 to April 2007. ① Detection of iNOS: The cells were seeded onto 24-well plate at the density of 1×105, After the cells had adhered to the cover glasses, the cells were grouped as negative control group (the primary antibody was replaced by phosphate buffered solution PBS); normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours). The expressions of iNOS protein were detected with immunocytochemical staining. ② Determination of iNOS and NF-κB gene activities: According to the introduction of the kit for transfection, iNOS or NF-κB report gene plasmids were transiently transfected with LipofectamineTM2000 liposomes into the cells in the 24-well plate for 24 hours. The cells were divided into normal control group (the cells were normally cultured after transfected with report gene plasmids); blank plasmid group (the cells were normally cultured after transfected with blank plasmids); LPS-treated group (the cells were treated with LPS for 4 hours after transfected with report gene plasmids); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours after transfected with report gene plasmids). The content of luciferase in the cell lysis buffer was determined after cell lysis. ③ Determination of SOD activity: The cells were seeded into culture bottle at the density of 1×106, and the divided into four groups, including normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours); vitamin C+LPS group (the cells were treated with vitamin C for 1 hour and LPS for 24 hours). The SOD activity was determined with xanthine oxidase and quantitative colorimetric assay. MAIN OUTCOME MEASURES: The expressions of iNOS protein, iNOS and NF-κB, and the activity of SOD were observed. RESULTS: ① Expression of iNOS protein in microglia: The expression of iNOS protein in the LPS-treated group was obviously higher than that in the negative control group (P < 0.01); Those in the curcumin+LPS group were significantly decreased as compared with that in the LPS-treated group (P < 0.01). ② Expressions of iNOS and NF-κB genes: The expressions of iNOS and NF-κB genes in the LPS-treated group were significantly higher than those in the normal control group (P < 0.01); Those in the curcumin+LPS group were significantly lower than those in the LPS-treated group (P < 0.01). ③ SOD activity: The activity of SOD in the LPS-treated group was significantly lower than those in the normal control group (P < 0.01). It in the curcumin+LPS group and vitamin C +LPS group was significantly higher than that in the LPS-treated group (P < 0.01). CONCLUSION: Curcumin could inhibit the expression of iNOS in the activated microglia, and it also has the abilities in eliminating free radicals and antagonizing lipid peroxidation.     

肝炎颗粒治疗急性病毒性肝炎91例临床总结

诊断标准按1995年北京传染病与寄生虫病学会修订《病毒性肝炎防治方案 (试行)》,共177例住院病人,乙型肝炎123例,甲型肝炎18例,戊型肝炎36例。分为治疗组91例,男69例,女22例,年龄 (35.5±10.4)岁 ;对照组86例,男70例,女16例,年龄 (34.6±12.3)岁。各组在性别、年龄、病史、病程及肝功能异常等方面无明显差异,具有可比性。