一氧化氮与卵巢生理

NO是一种结构简单的气体,是脊椎动物体内已发现的分子量最小的生物活性分子。NO作为一种信使分子,生物作用广泛,在生殖系统发挥重要作用。本文着重介绍NO作为卵巢内重要的调节因子,在调节卵泡发育、卵母细胞成熟、排卵、黄体的形成与退化、激素合成等方面的研究进展。     

乳胶增强免疫比浊试验测定血清胱抑素C

胱抑素C是一种含120个氨基酸残基多肽链的低分子的碱性蛋白.人体内所有的有核细胞均可持续合成CysC,并分泌到细胞外液,如血液、精液、脑脊液中[1].生理状态下,CysC产生比较恒定,不受年龄、性别、肌肉等因素的影响[2].     

尿卵泡刺激素联合人绒毛膜促性腺素治疗少弱畸精子综合征疗效观察

随着男科学研究的深入,传统的常规精液分析已不能对男性生育能力进行准确评价.为此,本课题组在尿卵泡刺激素(u-FSH)联合人绒毛膜促性腺激素(hCG)治疗少弱畸精子综合征(OATS)中,试行精子核成熟度检查,结果治疗后可使精子核成熟度增加,该法为疗效评价提供了又一种新途径.     

临床实验室检查结果解读性激素临床检测项目及意义

性激素在人体生殖系统中有着极为重要的作用，与人们的健康和部分疾病密切相关，直接影响着人们的生活质量和人生幸福。由于社会环境与自然环境的影响，性激素水平出现下降的趋势。现代医学研究表明，体内性激素水平的异常与冠心病、心肌梗死、乳腺增生、骨质疏松症等高危病的发病率有着明显的相关性，因此，对于性激素水平的研究具有极大的科研与临床意义。     

牛卵泡液对牛卵母细胞体外成熟的抑制作用

为了研究牛卵泡液对牛卵母细胞的抑制特性，收集了不同的发情期的小卵泡、中等卵 以及大卵泡的卵泡液，将卵泡液和从小卵泡吸取的胚泡期卵母细胞一起培养。２４小时后鉴定是否核成熟。牛卵泡液能够抑制牛卵母细胞的自发成熟。将卵母细胞培养液中的卵泡液去除后，抑制可以逆转。     

多囊卵巢综合征的研究进展

多囊卵巢综合征(Polycystic ovary syndrom e,PCOS)指青春期发病、卵泡膜细胞良性增生引起雄激素生成过多,造成月经紊乱、持续排卵障碍、高雄激素症、卵巢多囊性改变等表现,是育龄妇女内分泌紊乱中最常见的一种异质性疾病。近几年研究发现,PCOS不仅影响患者生殖功能,还存在多方     

放射线联合p53基因及内皮抑素治疗小鼠前列腺癌皮下移植瘤

Objective To test the hypothesis that p53 gene therapy combined with endostatin can enhance tumor response to radiation therapy of RM-1 mouse xenograft prostate cancer and to investigate its mechanism. Methods A mouse prostate cancer model was established. Then mice with xenograft tumor were randomly divided into group A (control), B (radiation), C (radiation and rAdp53), D (radiation and rh-endostatin) and E (radiation and rAdp53 and rh-endostatin). On day 1, rAdp53 was injected intra-tumorously with 1 × 1010 vp per animal to group C and E. From day 1 to 14, rh-endostatin was given 15 mg/kg intraperitoneally daily to group D and E. On day 4 single fraction of 15 Gy was given to tumors in groups B, C, D and E. Normal saline was injected intra-tumorously or intraperitoneaUy accordingly as control. No treatment was done to group A. Tumor volume was measured daily. Samples were collected on Days 5, 10 and 15. Ki67, CD31, p53 and VEGF were detected by means of immunohistochemistry. Results (1) Radiation alone, radiation combined with intra-tumorous injection of Adp53 and/or intraperitoneal injection of rh-endostatin resulted in tumor growth arrest of RM-1 cells in vivo (P = 0.000). Radiation combined with both rAdp53 and rh-endostatin was the most effective treatment (P < 0.05). (2) All the four treatment groups had a decreased expression of mutant type P53 (P = 0.000). The expression of Ki67 in groups B and C were equal (P 0.05) and increasing (P = 0.000), respectively. Group D had a up-down-up curve (P < 0.05), but group E had a up-down one. On day 5 the expresion of VEGF in group E was the lowest (P < 0.05). An increased expression of MVD compared with the control was shown, and MVD in groups C, D and E were always higher than that in the control (P < 0.05). Conclusions The limitation of radiotherapy could be overcome by combination with beth p53 gene therapy and endostatin on the growth of mouse prostate cancer cell. Radiation, rAdp53 and endostatin have their own role but they can be interacted with each other.     

儿童肥胖发病基因研究

肥胖是全世界所关注的问题 ,它不但影响了人的身体健康 ,也影响到人们的日常生活 ,而儿童的肥胖则是众多肥胖的类型之一 ,直接影响到儿童的身心健康。肥胖的病因极其复杂 ,包括环境、饮食及遗传等多种因素。本文主要从基因角度谈谈儿童肥胖的病因问题。1　ｏｂ基因与肥胖抑素 (ｌｅｐｔｉｎ)　　 1 994年有人利用定位克隆的方法成功克隆了小鼠的肥胖相关基因之一———ｏｂ基因 ,并且发现正是由于该基因突变造成的生理生化过程改变导致了小鼠的肥胖。ｏｂ基因序列在脊椎动物中具有高度的保守性 ,其定位于人类染色体 7ｑ3 1 .3上。ｏｂ基因在…     

人卵泡液中干细胞因子水平及其对卵泡发育的影响

目的 :探讨人卵泡液中是否存在干细胞因子 (SCF)及其与卵泡发育的关系 .方法 :采用夹心ELISA方法检测 3 0例进行体外受精 胚胎移植 (IVF ET)患者超促排卵前、取卵时血清与卵泡液中SCF的水平 .结果 :①取卵时卵泡液中SCF水平 (5 14± 67)ng·L 1,显著高于同期血清水平 (3 95± 4 9)ng·L 1,差别有显著性 (P <0 .0 5 ) ;②取卵时血清中SCF水平与超促排卵前血清中SCF水平相比 ,差别无显著性 [(3 95± 4 9)ng·L 1,(3 74± 3 6)ng·L 1,P >0 .0 5 ];③高、中反应型组卵泡中SCF水平 [(5 64± 64)ng·L 1,(5 3 2± 5 5 )ng·L 1]与低反应型 (3 5 2± 78)ng·L 1相比 ,差别有显著性 (P <0 .0 1) ,且与卵泡发育数目之间呈正相关 (r =0 .4 12 ,P <0 .0 5 ) .结论 :卵泡液中SCF可能来自于卵泡本身 ,而非血浆渗透 .SCF参与人卵泡发育的调控