STUDY ON THE MECHANISM AND EFFECTS OF GLY-TYR-NH2 AND GLY-TYR-LYS ON RAT LUTEAL CELLS IN VITRO

The action of Gly-Tyr-NH2,(GY-NH2) and Gly-Tyr-LYS(GYK) on ^125I-LH binding,cAMP accumulation and progesterone production was investigated.Incubation of rat luteal cells for 2.5h with GH-NH2 and GYK at dosage of 0.2mmol/L caused a significant inhibition of basal and gonadotropin-stimulated steroidogenesis.GYNH2 and GYK were also foumnd to reduce cAMP formation in response to hCG.The activity of adenylate cyclase of luteal cells was inhibitd by 0.2mmol/LGY-NH2 and GYK.GY-NH2 and GYK at a concentration of 0.2mmol/L were not found to have an inhibitory effect on 8Br-cAMP-stimulated progesterone production.GY-NH2 and GYK did not affect ^125I-LH binding to LH receptors on the luteal cellsurface.These results suggest that GY-NH2 and GYK inhibit steroidogenesis at the step of gonadotropin-stimulated c AMP for mation in luteal cells.Adenylate cyclase in luteal cells was also inhibited.     

改革外科手术学教学 培养实用型人才

针对我院外科手术学教学目前存在的实验动物资源匮管乏、实验室条件尚不完善、学生实践机会较少等直接影响教学进度和安排，有碍于教学质量的提高等主要问题，我们经过积极探索和可行性实验，着手于开发实验动物资源、节约经费、改善教学条件、加强教学环节和建立考核制度的改革，以促进教学质量的提高，为培养实用型人才创造条件。     

川芎嗪和维拉帕米对缺血性急性肾衰初期保护效应的实验研究

１８只犬切除右肾，钳夹左肾动脉６０分的肾缺血模型，再灌注５小时。将动物随机分成对照组、川芎嗪组及维拉帕米组。观察尿量、菊糖清除率、利钠指数、平均动脉压及心率；再灌注５小时取肾组织作形态学检查。结果显示川芎嗪对肾功能及形态有保护作用，维拉帕米则无此作用，提示在手术中有肾缺血时以川芎嗪作为肾保护药物优于维拉帕米。     

辐照灭菌动物腹膜覆盖烧伤创面的研究和临床应用

本文对动物腹膜辐照灭菌方法、抗菌和水分蒸发试验进行了研究,临床应用56例烧伤病人,取得了满意效果。对辐照动物腹膜覆盖烧伤创面优点进行了讨论。     

2004-2005年襄樊市动物致伤分析

狂犬病是一种由狂犬病毒引起的人兽共患传染病,是迄今为止人类病死率最高的急性传染病,一旦发病,病死率高达100%[1].我国狂犬病的发病数仅次于印度,居世界第2位[2].狂犬病毒的主要宿主为野生动物.我国狂犬病的宿主动物和传染源主要是犬,其次是猫、鼠和狐狸等[3].为了解襄樊市能引发狂犬病的动物致伤情况,对动物致伤后主动进行处理的3 215名例患者做了调查,并对相关危险因素进行分析,结果如下.……