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191.
Correlation between changes of central neurotransmitter expression and stress response in mice*☆
A restraint time-course analysis 总被引:1,自引:0,他引:1
BACKGROUND: Changes in central neurotransmitter expression play an important role in stress response and forms the basis for stress-induced psychological and behavior changes.
OBJECTIVE: To observe the effects of different restraint stress intervals on brain monoamine neurotransmitter expression, and to investigate the correlation between stress response and neurotransmitter levels.
DESIGN: Randomized controlled animal study.
SETTING: Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University.
MATERIALS: Sixty 7-week-old male Kunming mice of clean grade, weighing 18-22 g, were provided by the Guangdong Medical Experimental Animal Center. The experiment was in accordance with animal ethics standards.
METHODS: This study was performed at the Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University from June 2006 to May 2007. A restraint device for mice was constructed according to published reports. Experimental mice were adaptively fed for 1 week and randomly divided into a control group (n = 10) and an experimental group (n = 50). The experimental group was sub-divided into five restraint intervals: 4, 8, 12, 18, and 24 hours (n = 10 mice per time point). Animals in the experimental group were not allowed to eat or drink during the restraint period. Mice in the control group did not undergo restraint, but had identical food and water restrictions. Cerebral cortex and hypothalamus were separated based on observational times and protein was extracted using perchloric acid. Central monoamine neurotransmitter levels were measured using high performance liquid chromatography with electrochemical detection. MAIN OUTCOME MEASURES: Levels of norepinephrine (NE), dopamine hydrochloride (DA), 3,4-dihydroxyphen-ylanetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleac-etic acid (5-HIAA) in the cerebral cortex and hypothalamus of mice. RESULTS: Sixty mice were i 相似文献
OBJECTIVE: To observe the effects of different restraint stress intervals on brain monoamine neurotransmitter expression, and to investigate the correlation between stress response and neurotransmitter levels.
DESIGN: Randomized controlled animal study.
SETTING: Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University.
MATERIALS: Sixty 7-week-old male Kunming mice of clean grade, weighing 18-22 g, were provided by the Guangdong Medical Experimental Animal Center. The experiment was in accordance with animal ethics standards.
METHODS: This study was performed at the Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University from June 2006 to May 2007. A restraint device for mice was constructed according to published reports. Experimental mice were adaptively fed for 1 week and randomly divided into a control group (n = 10) and an experimental group (n = 50). The experimental group was sub-divided into five restraint intervals: 4, 8, 12, 18, and 24 hours (n = 10 mice per time point). Animals in the experimental group were not allowed to eat or drink during the restraint period. Mice in the control group did not undergo restraint, but had identical food and water restrictions. Cerebral cortex and hypothalamus were separated based on observational times and protein was extracted using perchloric acid. Central monoamine neurotransmitter levels were measured using high performance liquid chromatography with electrochemical detection. MAIN OUTCOME MEASURES: Levels of norepinephrine (NE), dopamine hydrochloride (DA), 3,4-dihydroxyphen-ylanetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleac-etic acid (5-HIAA) in the cerebral cortex and hypothalamus of mice. RESULTS: Sixty mice were i 相似文献
192.
钙吸收率分析方法研究 总被引:2,自引:0,他引:2
钙是人体内最重要的矿物元素,钙缺乏会导致多种疾病,选择有效补钙制剂是预防和治疗钙缺乏的重要手段。但是,市场上补钙制剂种类繁多,质量参差不齐,分析钙吸收率的方法显得尤为重要。本文对吸收率有关概念定义及吸收率和摄取量的关系作了介绍。提出确定钙的摄入量是分析钙吸收率的前提条件,摄入量过低或过高均不能对钙吸收作出客观的评价。分析了目前各钙剂评价方法优缺点并结合我们用^41Ca示踪法对钙吸收率分析方法研究作了系统的阐述。结论表明。^41Ca分析钙吸收率方法是目前较科学有效的方法,该方法通过研究成熟后将会发展为分析钙吸收率的标准方法。 相似文献
193.
194.
《山东医药》2009,(47):98-98
统计学分析方法的选择:对于定量资料,应根据所采用的设计类型、资料所具备的条件和分析目的,选用合适的统计学分析方法,不应盲目套用t检验和单因素方差分析;对于定性资料,应根据所采用的设计类型、定性变量的性质和频数所具备的条件以及分析目的,选用合适的统计学分析方法,不应盲目套用,检验。对于回归分析,应结合专业知识和散布图,选用合适的回归类型,不应盲目套用简单直线回归分析,对具有重复实验数据的回归分析资料,不应简单化处理;对于多因素、多指标资料,要在一元分析的基础上,尽可能运用多元统计学分析方法,以便对因素之间的交互作用和多指标之间的内在联系进行全面、合理的解释和评价。 相似文献
196.
197.
替硝唑在离子液体-碳纳米管复合修饰电极上的电催化还原及电分析方法 总被引:1,自引:1,他引:0
目的研究替硝唑(TNZ)在玻碳电极(GCE),多壁碳纳米管修饰电极(MWCNTs/GCE)及疏水性室温离子液体(RTIL)1-丁基-3-甲基咪唑六氟磷酸盐(BMIMPF6)-多壁碳纳米管(MWCNT)修饰电极(RTIL-MWCNTs/GCE)上的电化学行为,电化学动力学性质及电化学定量分析方法。方法运用循环伏安法(CV)、计时库仑法(CC)、计时电流法(CA)、方波伏安法(SWV)、稳态电流-时间曲线及电化学交流阻抗谱。结果与GCE相比,TNZ在RTIL-MWCNTs/GCE上的还原峰电位正移了105 mV,还原峰电流增大了约5倍;与MWCNTs/GCE相比,其还原峰电位稍有负移,但还原峰电流增大。TNZ在RTIL-MWCNTs/GCE上的还原峰电流与浓度在5.0×10^-5-1.0×10^-2 mol.L^-1内呈良好线性关系,检出限为2.4×10^-6 mol.L^-1。加标回收率在98.7%-100.2%之间,RSD在0.64%-1.65%之间。结论RTIL-MWCNTs/GCE对TNZ电化学还原具有良好的催化作用,是一受扩散控制的不可逆电极反应过程,该方法可用于TNZ含量的电化学定量测定,操作简便快捷,测定结果符合定量测定要求。 相似文献
198.
199.
多肽类药物作为药物研究的新课题越来越多地受到了人们的关注,其分析手段的研究也成为了最活跃、发展最迅速的领域之一。目前,建立灵敏、准确、操作简便的多肽类药物分析方法面临着诸多挑战和机遇。本文中,作者首先介绍了几种常见的多肽类药物及其主要优势,进而介绍了几种备受关注的多肽类药物的分析方法。 相似文献
200.