大鼠延髓和脊髓背角内PKCγ阳性神经元向中缝大核的投射

目的 研究大鼠延髓背角和脊髓背角浅层内蛋白激酶Cγ亚单位（PKCγ）阳性神经元向中缝大核（NRM）的投射。方法 荧光金（FG）逆行追踪和PKCγ免疫荧光组织化学染色相结合的双标记方法。结果 将FG注入NRM，在延髓和脊髓背角的Ⅰ-Ⅲ层内可见FG逆标神经元；PKCγ阳性神经元主要分布于延髓和脊髓背角的Ⅱ层内侧部及Ⅱ、Ⅲ层交界处，Ⅰ、Ⅲ层内较少；延髓背角Ⅰ层和脊髓背角Ⅰ－Ⅲ层可观察到FG逆标并呈PKCγ阳性的双标神经元。结论 延髓和脊髓背角浅层向NRM投射的神经元含PKCγ，提示PKCγ在向NRM传递痛信息的通路中可能发挥重要作用。     

充血性心力衰竭病人甲状腺激素的变化及其意义

目的：观察充血性心力衰竭病人血清T3、T4水平变化及其与心脏功能之间的关系。方法：采集充血性心力衰竭病人的血标本。用放射免疫方法测定血清T3、T4水平。结果：与正常对照组相比充血性心力衰竭病人血清T3水平显降低，并与患的心脏功能有密切的关系。结论：充血性心力衰竭病人血清T3水平显低于正常人水平，其机制可能是由于T4向T3转化速度减慢所致。较低的血清T3水平可以作为充血性心力衰竭病人不良预后的指标之一。     

宫外孕术后并发急性非少尿型肾功能衰竭1例报告

1病例 患者，36岁。孕4产2。因停经2月，左下腹撕裂样痛3小时，于2003年9月5日15时40分急诊入院。     

中医药防治高血压病的特色

心脑血管疾病是中老年人健康的主要杀手之一,高血压病就是杀手背后的黑手.如何发挥中医药在防治高血压病中的特色和优势,是我们中医药工作者义不容辞的任务.中医治疗高血压的特色主要有以下几点:     

脑梗死患者血清维生素B_(12)与叶酸水平的变化

脑血管疾病是多因异质性疾病,近年来研究表明同型半胱氨酸(hymocyste ine,HCY)可能是导致动脉粥样硬化的独立危险因素。营养障碍可影响血液中HCY的水平,维生素B12与叶酸水平的变化可引起HCY水平的变化,导致动脉粥样硬化,最终引起脑卒中[1]。我们检测了脑梗死患者血清维生素B12和叶酸水平,了解其变化与脑梗死之间的关系。一、材料和方法1.研究对象(1)脑梗死组:36例,均来自我院住院患者,男25例,女11例,年龄53~70岁,所有患者经头部CT和/或MR I检查确诊,符合全国第4届脑血管疾病会议的诊断标准[2],并排除心源性脑梗死、脑脉管炎、血液病…     

血绒毛膜促性腺激素β亚单位在早期诊断异位妊娠中的作用

异位妊娠又名宫外孕，是指受精卵种植在子宫腔以外部位的妊娠。近年来国内外文献报道异位妊娠发病率呈上升趋势，可能与性病、流产等高危因素增加、助孕技术广泛开展等有关。异位妊娠在早期与先兆流产有时不易鉴别，为了探讨血绒毛膜促性腺激素β亚单位（β-hcG）值在早期诊断异位妊娠中的价值，我们对比分析研究了同期住院的异位妊娠和先兆流产患者的血β-hcG值。     

妇女叶酸增补中需要关注的问题

妇女围孕期增补叶酸能够有效预防神经管缺陷等疾病的发生。随着对叶酸研究的不断深入，叶酸补充中出现了诸多问题。本文简要综述了叶酸在服用方式以及基础和临床研究中出现的值得讨论、已经引起关注和需要进一步研究探讨的问题，并提出了深入研究叶酸可能的发展趋势。     

急救止痛用好合谷

合谷穴在手背，第一、二掌骨之间，约平第二掌骨中点处，是人体的重要穴位“四总穴”之一，主治头面部的各种疾患。一般老百姓若会使用它，可在不少场合派上用场。外出旅行或节假日期间，生活规律常被打乱，很多意想不到的急症可随时发生。这时，您不妨试试应用合谷穴来解除急症。     

Changes in T .lymphocyte subsets after severe traumatic brain inJury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.     

新型纳米根管充填材料对成骨细胞生长的影响

通过体外培养的成骨细胞,采用二甲基噻唑二苯基四唑溴盐比色法和流式细胞术对新型纳米根管充填材料(nHA-PA66)作用下的成骨细胞生长情况的变化进行研究,评价其对成骨细胞生长的影响。以该材料的细胞培养基浸提液作用于实验组细胞,对照组采用培养基本身。实验组和对照组成骨细胞的生长情况和细胞周期无显著性差异,表明该新型纳米材料对成骨细胞的生长和细胞周期无不良影响。提示新型纳米根管充填材料的成骨细胞相容性较好,具有用作根充材料的基础。