A Novel Hypoxia-inducible Factor 1α Inhibitor KC7F2 Attenuates Oxygen-induced Retinal Neovascularization

PurposeKC7F2 is a novel molecule compound that can inhibit the translation of hypoxia-inducible factor 1α (HIF1α). It has been reported to exhibit potential antiangiogenic effect. We hypothesized that KC7F2 could inhibit oxygen-induced retinal neovascularization (RNV). The purpose of this study was to investigate this assumption.MethodsOxygen-induced retinopathy (OIR) models in C57BL/6J mice and Sprague-Dawley rats were used for in vivo study. After intraperitoneal injections of KC7F2, RNV was detected by immunofluorescence and hematoxylin and eosin staining. Retinal inflammation was explored by immunofluorescence. EdU incorporation assay, cell counting kit-8 assay, scratch test, transwell assay, and Matrigel assay were used to evaluate the effect of KC7F2 on the proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVEC) induced by vascular endothelial growth factor (VEGF) in vitro. Protein expression was examined by Western blot.ResultsKC7F2 treatment (10 mg/kg/d) in OIR mice significantly attenuated pathological neovascularization and decreased the number of preretinal neovascular cell nuclei, without changing the avascular area, which showed the same trends in OIR rats. Consistently, after the KC7F2 intervention (10 µM), cell proliferation was inhibited in VEGF-induced HUVEC, which was in agreement with the trend observed in the retinas of OIR mice. Meanwhile, KC7F2 suppressed VEGF-induced HUVEC migration and tube formation, and decreased the density of leukocytes and microglia colocalizing neovascular areas in the retinas. Moreover, the HIF1α–VEGF pathway activated in retinas of OIR mice and hypoxia-induced HUVEC, was suppressed by KC7F2 treatment.ConclusionsThe current study revealed that KC7F2 was able to inhibit RNV effectively via HIF1α–VEGF pathway, suggesting that it might be an effective drug for RNV treatment.     

2型糖尿病合并无症状性心肌缺血患者脂联素、E-选择素及CD11b/CD18的改变

选择36例正常人、36例2型糖尿病伴无症状性心肌缺血患者、38例2型糖尿病不伴心肌缺血患者,测定血中脂联素、E-选择素及CD11b/CD18活性的改变,并分析与血糖、血脂等因素的相关性。结果显示,与其他两组比较,2型糖尿病伴无症状心肌缺血组E-选择素及CD11b/CD18明显升高,而脂联素水平降低;脂联素与糖化血红蛋白、甘油三酯、E-选择素、CD11b/CD18呈负相关,与HDL呈正相关;E-选择素、CD11b/CD18与糖化血红蛋白呈正相关。认为脂联素活性降低及E-选择素、CD11b/CD18水平升高参与了2型糖尿病无症状性心肌缺血的发生。     

反油酸对人脐静脉平滑肌细胞活力的影响

<正>平滑肌细胞是血管壁中膜的主要细胞成分,是动脉粥样硬化(atherosclerosis,As)和血管成形术后再狭窄(restenosis after angioplasty,RAA)等病理过程形成和发展的重要部位,对血管壁的完整性和紧张性的调节也起重要作用,其异常增殖及形态学转变将导致动脉粥样硬化过程     

The role of electronic medical records in improving health care quality: A quasi-experimental study

The Teleprimary Care—Oral Health Clinical Information System (TPC-OHCIS) is an updated electronic medical record (EMR) that has been applied in Malaysian primary healthcare. Recognizing the level of patient satisfaction following EMR implementation is crucial for assessing the performance of health care services. Hence, the main objective of this study was to compare the level of patient satisfaction between EMR-based clinics and paper-based clinics.The study was a quasi-experimental design that used a control group and was conducted among patients in 14 public primary healthcare facilities in the Seremban district of Malaysia from May 10, to June 30, 2021. Patient satisfaction was assessed using the validated Short-Form Patient Satisfaction Questionnaire, which consisted of 7 subscales. All data were analyzed using the IBM Statistical Package for Social Sciences version 21.A total of 321 patients consented to participate in this study, and 48.9% of them were from EMR clinics. The mean score for the communication subscale was the highest at 4.08 and 3.96 at EMR-adopted clinics and paper-based record clinics. There were significant differences in general satisfaction and communication subscales, with higher patient satisfaction found in clinics using EMR.With the utilization of EMR, patient satisfaction and communication in delivering healthcare services have improved.     

CK18-M30和内毒素在大鼠非酒精性脂肪肝病中的促进作用

目的探讨细胞角蛋白18-M30片段(CK18-M30)、内毒素在大鼠非酒精性脂肪肝病(NAFLD)转化为非酒精性脂肪性肝炎(NASH)中的促进作用。方法 26只大鼠随机分为3组,对照组(NC组):普通饮食;NAFLD组:高脂饮食喂养8周;NASH组:高脂饮食喂养12周。生化仪测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST);光度法测定血清内毒素(ET);ELISA检测血清IL-18、CK18-M30,RT-PCR分析粪便肠道菌群;行苏木精伊红(HE)染色观察肝脏及末端回肠组织病理。结果 NAFLD及NASH组相比NC组,小肠黏膜结构由轻到重受到破坏。NAFLD组及NASH组大鼠血清ET、CK18-M30、肝脂肪变性程度及NAFLD活动度积分(NAS)、大肠杆菌属计数显著高于NC组,而双歧杆菌属、乳酸杆菌属计数显著低于NC组,差异有统计学意义(P<0.05);NASH组大鼠血清ET、CK18-M30、肝脂肪变性程度及NAS显著高于NAFLD组,差异有统计学意义(P<0.05);NASH组大鼠血清ALT、AST、IL-18显著高于NC组及NAFLD组,差异有统计学意义(P&l...     

SSEP and F-wave studies in acute inflammatory demyelinating polyradiculoneuropathy

Somatosensory evoked potentials (SSEPs) and F-wave responses were compared after tibial (PTN) and median (MN) nerve stimulation in patients with acute inflammatory demyelinating polyradiculoneuropathy (AIDP). Nineteen patients were evaluated within 2 weeks of AIDP onset. Each had F-wave and PTN-SSEP studies; 18 had MN-SSEP studies. Ten patients had absent or prolonged MN-SSEP latencies, whereas 16 had abnormal MN F-wave studies. Seventeen patients had an abnormal PTN-SSEP while 12 had abnormal PTN F waves. Every patient with an abnormal MN-SSEP also had an abnormal PTN-SSEP. The most common PTN-SSEP findings were an absent or prolonged latency of N8 or N22. All patients had either an abnormal MN F wave or PTN-SSEP.     

18F-FDG PET/CT在不明原发灶肿瘤中的临床应用价值

目的探讨18F-FDG PET/CT全身显像在不明原发灶肿瘤（CUP）诊断中的临床应用价值。方法回顾分析46例于2015年2月至2016年6月在我院行常规检查未能发现肿瘤原发灶而进一步行18F-FDG PET/CT全身显像查找肿瘤原发灶的转移性肿瘤患者的资料。PET/CT图像分析采用视觉及半定量分析方法。通过病理活检和（或）临床综合诊断、临床随访对结果进行评价。结果46例患者中,18F-FDG PET/CT显像找到原发肿瘤33例,均经过病理活检及临床随访证实;13例未发现原发病灶。18F-FDG PET/CT对不明肿瘤原发灶的检出率为71.7%（33/46）,其中阳性患者中淋巴瘤3例、胃癌2例、食管癌4例、卵巢癌3例、肺癌14例、肝癌2例、尿路上皮癌1例、鼻咽癌2例、多发性骨髓瘤1例、降结肠癌1例。转移方式主要有淋巴结转移32例、骨转移20例、肝转移13例、肺转移9例、胸膜腹膜转移5例、肾上腺转移3例、脑转移4例、皮下转移3例、心包膜转移1例。结论18F-FDG PET/CT全身显像对CUP的检出率显著优于一般常规检查,对临床指导治疗有着重要意义。     

两种多位点探针的制备及AKR和NOD小鼠DNA指纹分析

用所制备的复制型M13mp18和寡核苷酸(GACA)_4探针,分别与近交系AKR小鼠的9个个体及NOD小鼠的5个个体进行DNA杂交,结果提示,一种探针和近交系内各个体杂交的DNA指纹图完全一致;同一探针与不同近交系小鼠杂交的指纹图完全不同;不同探针与同一近交系小鼠杂交的指纹图也完全不同。说明用DNA指纹图分析,对建立各种实验动物标准的DNA指纹图,用于遗传监测、杂交育种、基因制图以及寻找遗传标记等均有实用价值和参考作用。